##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062758_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1547625
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24673742024069	32.0	32.0	32.0	32.0	32.0
2	30.867467248202892	32.0	32.0	32.0	32.0	32.0
3	30.91934609482271	32.0	32.0	32.0	32.0	32.0
4	30.983318310314193	32.0	32.0	32.0	32.0	32.0
5	30.90408787658509	32.0	32.0	32.0	32.0	32.0
6	34.55559324771828	36.0	36.0	36.0	32.0	36.0
7	34.481055811323806	36.0	36.0	36.0	32.0	36.0
8	34.468761489378885	36.0	36.0	36.0	32.0	36.0
9	34.595273402794604	36.0	36.0	36.0	32.0	36.0
10	34.32147193280026	36.0	36.0	36.0	32.0	36.0
11	34.576235199095386	36.0	36.0	36.0	32.0	36.0
12	34.39097455779016	36.0	36.0	36.0	32.0	36.0
13	34.485801793070024	36.0	36.0	36.0	32.0	36.0
14	34.37948469428964	36.0	36.0	36.0	32.0	36.0
15	34.32602406913819	36.0	36.0	36.0	32.0	36.0
16	34.33902011146111	36.0	36.0	36.0	32.0	36.0
17	34.26208157660932	36.0	36.0	36.0	32.0	36.0
18	34.28594653097488	36.0	36.0	36.0	32.0	36.0
19	34.27361150149422	36.0	36.0	36.0	32.0	36.0
20	34.25961909377271	36.0	36.0	36.0	32.0	36.0
21	34.24361133995639	36.0	36.0	36.0	32.0	36.0
22	34.2096780550844	36.0	36.0	36.0	32.0	36.0
23	34.15641450609805	36.0	36.0	36.0	32.0	36.0
24	34.14161440917535	36.0	36.0	36.0	32.0	36.0
25	34.117834746789434	36.0	36.0	36.0	32.0	36.0
26	34.05622292221953	36.0	36.0	36.0	32.0	36.0
27	34.06615814554559	36.0	36.0	36.0	32.0	36.0
28	34.05058395929247	36.0	36.0	36.0	32.0	36.0
29	34.01876682012762	36.0	36.0	36.0	32.0	36.0
30	33.99337307164203	36.0	36.0	36.0	32.0	36.0
31	34.012482351990954	36.0	36.0	36.0	32.0	36.0
32	33.961944915596476	36.0	36.0	36.0	32.0	36.0
33	33.920171230110654	36.0	36.0	36.0	32.0	36.0
34	33.93367967046281	36.0	36.0	36.0	32.0	36.0
35	33.887151603263064	36.0	36.0	36.0	32.0	36.0
36	33.83737145626363	36.0	36.0	36.0	32.0	36.0
37	33.85215346094823	36.0	36.0	36.0	32.0	36.0
38	33.24319457232857	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	5.0
9	13.0
10	13.0
11	17.0
12	6.0
13	9.0
14	176.0
15	380.0
16	474.0
17	593.0
18	756.0
19	1013.0
20	1433.0
21	2009.0
22	3022.0
23	4476.0
24	6900.0
25	10192.0
26	15034.0
27	21371.0
28	29973.0
29	40620.0
30	54825.0
31	74161.0
32	100844.0
33	150713.0
34	332280.0
35	696312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28472651711387	17.272364992327418	11.603258970968401	25.839649519590314
2	17.04506442901559	19.797561453404285	36.92596239447975	26.23141172310038
3	18.311492932188557	24.23761081219409	28.530060655095713	28.920835600521638
4	12.55448850911168	15.254391712447998	35.80759304563142	36.3835267328089
5	15.144589570321434	36.13737721484962	32.922574116935536	15.795459097893414
6	34.3122786113886	35.4259901345164	16.84685755787281	13.414873696222193
7	30.673451255956707	30.22754220176076	21.040949842500606	18.058056699781925
8	28.81115212042264	31.500126646403242	19.70456495668176	19.98415627649236
9	28.08789254705783	13.95041538054958	18.53798452717953	39.42370754521306
10	16.560449053992322	25.498947085471848	30.36290174068515	27.577702119850684
11	38.20775199408624	20.67174730029414	22.097266951671518	19.02323375394811
12	25.580310381971298	23.321165925831156	27.845722700712145	23.2528009914854
13	29.46677954491111	18.59065910985595	25.30086145057161	26.64169989466133
14	24.572690720490296	19.674514725287793	24.229292382295277	31.523502171926637
15	25.789924743219522	26.42764258329046	22.034259802641614	25.7481728708484
16	26.779127386839203	24.848847518876287	23.127492820418706	25.244532273865804
17	24.604323056191976	25.163625317895537	24.803510665455946	25.42854096045654
18	26.071950832585365	23.66811865884258	25.67144137525048	24.588489133321573
19	26.320792514281283	24.224222607077312	24.983521596401893	24.47146328223951
20	26.459521127798126	23.184132455278185	24.286806361958007	26.069540054965685
21	28.354704071867197	23.412800214019715	23.471086983633825	24.761408730479264
22	26.617766416112655	23.33829185578842	24.262619030386997	25.78132269771193
23	24.562980501734767	23.040690261723807	25.082085544737186	27.31424369180424
24	25.30187909843084	24.45639143564878	24.538271193253678	25.7034582726667
25	25.60687784115511	23.524408891094815	24.582801778012158	26.285911489737913
26	25.301084129061447	24.710839674922852	25.181522950899133	24.80655324511657
27	26.19681521335951	23.942184787932913	24.03039259907385	25.830607399633728
28	25.027721845848443	23.75846194805094	25.271984263813753	25.941831942286864
29	24.902194437587323	24.101138770809936	25.291995000833623	25.70467179076912
30	24.8914259276371	24.358716716174676	25.50090109533647	25.248956260851752
31	25.834863191578318	24.176283610840247	23.73461175848724	26.254241439094194
32	25.303843340389843	24.19192663301256	23.608343220196602	26.895886806401
33	24.855749067675966	23.61689382136213	24.764374527052745	26.76298258390916
34	25.96075800814773	23.91173976880286	24.83669634179126	25.290805881258144
35	27.071934960610584	23.501231121192735	24.676515636208535	24.75031828198815
36	25.24515664562893	24.708506906521873	23.93041648517686	26.11591996267234
37	26.503359035534174	24.41620763725001	24.190871798318287	24.889561528897527
38	25.37253249724074	23.929768739063118	24.732474494609423	25.96522426908672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	311.0
1	297.5
2	284.0
3	284.0
4	578.0
5	872.0
6	872.0
7	1040.5
8	1209.0
9	1124.0
10	1039.0
11	1039.0
12	1311.0
13	1583.0
14	2025.5
15	2468.0
16	2468.0
17	3760.5
18	5053.0
19	5053.0
20	6115.0
21	7177.0
22	7777.5
23	8378.0
24	8378.0
25	9784.0
26	11190.0
27	11190.0
28	14769.5
29	18349.0
30	22883.0
31	27417.0
32	27417.0
33	37746.0
34	48075.0
35	48075.0
36	53618.5
37	59162.0
38	69588.0
39	80014.0
40	80014.0
41	85158.0
42	90302.0
43	104702.5
44	119103.0
45	119103.0
46	122165.0
47	125227.0
48	125227.0
49	135296.0
50	145365.0
51	147626.5
52	149888.0
53	149888.0
54	142204.5
55	134521.0
56	134521.0
57	131891.5
58	129262.0
59	116193.5
60	103125.0
61	103125.0
62	101270.5
63	99416.0
64	81740.5
65	64065.0
66	64065.0
67	53513.5
68	42962.0
69	42962.0
70	34914.0
71	26866.0
72	21036.5
73	15207.0
74	15207.0
75	11420.5
76	7634.0
77	7634.0
78	7863.5
79	8093.0
80	6325.0
81	4557.0
82	4557.0
83	4700.0
84	4843.0
85	4843.0
86	3201.5
87	1560.0
88	1360.5
89	1161.0
90	1161.0
91	822.5
92	484.0
93	432.5
94	381.0
95	381.0
96	342.0
97	303.0
98	303.0
99	511.0
100	719.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12024876827396817
2	0.05711978030853728
3	0.012083030449882886
4	0.00329537194087715
5	1.9384540828689122E-4
6	4.523059526694128E-4
7	0.0
8	5.815362248606736E-4
9	5.169210887650432E-4
10	0.0010338421775300864
11	0.0031661416686858896
12	0.0031015265325902595
13	0.015184556982473143
14	0.009756885550440192
15	0.025135287941200225
16	0.011695339633309102
17	0.021969146272514336
18	0.006203053065180519
19	0.009369194733866408
20	0.007366125514901865
21	0.006719974153945562
22	0.007818431467571278
23	0.009950730958727082
24	0.015119941846377513
25	0.018027622970680883
26	0.01938454082868912
27	0.009239964461675148
28	0.007043049834423713
29	0.010790727727970278
30	0.004135368710120346
31	0.006203053065180519
32	0.007559970923188756
33	0.0095630401421533
34	0.01298764235522171
35	0.016476859704385755
36	0.01595993861562071
37	0.010144576367013972
38	0.007043049834423713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1547625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.889932451898076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13664234852799	33.41632089791216
2	13.138226588114962	11.53271754980194
3	4.553791141495492	5.995967568008671
4	1.9821834074935756	3.4799158344866474
5	1.0572630514615444	2.3201601956267406
6	0.6470331412022085	1.7038944516902619
7	0.4210541961386344	1.2936028158979034
8	0.30072567879766854	1.0559063783184697
9	0.23296249674290923	0.9202237421284629
>10	1.2830897677831625	10.167310375843106
>50	0.10351691351864552	3.194264559293837
>100	0.11224267065835314	10.95325849150252
>500	0.01961662048413291	5.893904598302145
>1k	0.011651977580800749	8.072552541187234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3188	0.20599305387286973	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2864	0.18505774977788547	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2854	0.18441159841692917	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2769	0.17891931184880058	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2740	0.1770454729020273	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2628	0.1698085776593167	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2501	0.16160245537517162	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2428	0.15688555044019062	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2413	0.15591632339875616	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2326	0.1502948065584363	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2279	0.14725789516194168	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2209	0.14273483563524755	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2191	0.1415717631855262	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2180	0.14086099668847427	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2124	0.13724254906711897	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2029	0.13110411113803408	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2018	0.13039334464098215	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2017	0.1303287295048865	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2006	0.12961796300783457	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1978	0.12780873919715693	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1921	0.124125676439706	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1885	0.1217995315402633	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1850	0.11953800177691624	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1822	0.11772877796623858	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1761	0.11378725466440513	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1753	0.11327033357564009	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1664	0.107519586463129	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1660	0.10726112591874647	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1658	0.1071318956465552	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1597	0.10319037234472175	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1571	0.10151037880623537	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1571	0.10151037880623537	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1550	0.10015346094822712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.46151360956304E-5	0.0
9	0.0	0.0	0.0	1.292302721912608E-4	0.0
10	0.0	0.0	0.0	1.292302721912608E-4	0.0
11	0.0	0.0	0.0	1.292302721912608E-4	0.0
12	0.0	0.0	0.0	1.292302721912608E-4	0.0
13	0.0	0.0	0.0	1.292302721912608E-4	0.0
14	0.0	0.0	0.0	1.292302721912608E-4	0.0
15	0.0	0.0	0.0	1.292302721912608E-4	0.0
16	0.0	0.0	0.0	1.292302721912608E-4	0.0
17	0.0	0.0	0.0	1.292302721912608E-4	0.0
18	0.0	0.0	0.0	1.292302721912608E-4	0.0
19	0.0	0.0	0.0	1.292302721912608E-4	0.0
20	0.0	0.0	0.0	1.938454082868912E-4	6.46151360956304E-5
21	0.0	0.0	0.0	3.876908165737824E-4	1.292302721912608E-4
22	0.0	0.0	0.0	7.753816331475648E-4	1.292302721912608E-4
23	0.0	0.0	0.0	0.0013569178580082385	1.292302721912608E-4
24	0.0	0.0	0.0	0.002067684355060173	1.292302721912608E-4
25	0.0	0.0	0.0	0.0025199903077295855	1.292302721912608E-4
26	0.0	0.0	0.0	0.0027784508521121075	1.292302721912608E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	75	6.4137566E-8	21.330877	3
CGTATAC	40	0.0044871564	19.9977	3
TTAGGAC	145	6.91216E-11	16.549818	3
GATATAA	80	5.5788296E-5	16.014717	1
GTACTAA	70	3.6716383E-4	16.014717	1
CCCTATA	70	3.688854E-4	16.00488	2
TATAGGG	70	3.688854E-4	16.00488	2
ACCGTCG	450	0.0	15.999193	8
TAGGACG	60	0.002444823	15.997642	4
GTTCAAA	685	0.0	15.897821	1
ATCGTTT	470	0.0	15.318376	29
TCTAGCG	580	0.0	15.171647	28
GTATTAG	460	0.0	14.970279	1
CTAGCGG	595	0.0	14.789169	29
CTAAGGT	130	8.330244E-8	14.767054	4
TACCGTC	500	0.0	14.719256	7
TAGCGGC	600	0.0	14.6664	30
CATCGTT	495	0.0	14.544721	28
TAGACAG	165	4.8567017E-10	14.54284	5
CGAGCCG	695	0.0	14.503802	15
>>END_MODULE