FastQCFastQC Report
Thu 2 Feb 2017
SRR4062758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547625
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35830.23151603263064374No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31660.20457152087876587No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA30480.19694693481948147No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28850.1864146676358937No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28260.1826023746062515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26910.1738793312333414No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25800.16670705112672643No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25760.1664485905823439No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25700.16606089976577013No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25290.16341167918584928No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24350.15733785639286002No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24270.156820935304095No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22670.1464825135287941No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22440.14499636539859462No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21830.14105484209676117No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21380.1381471609724578No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21300.13763023988369275No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21160.13672562797835394No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG20000.1292302721912608No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT19350.12503028834504482No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19320.12483644293675793No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19200.12406106130361037No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT18960.12251029803731525No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC18950.12244568290121963No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG18930.12231645262902836No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18270.11805185364671675No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18160.1173410871496648No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG17150.11081495840400614No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA16830.10874727404894596No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16020.1035134480251999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15850.10241499071157419No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15740.10170422421452226No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT15700.10144576367013974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACA1250.020.47942
ACTAGAC751.5092119E-619.2000583
GTATAAA1351.8189894E-1218.963021
GTATTAT1301.2732926E-1118.4615961
TTATACT904.3690125E-717.7778324
TCTAGCG5500.017.45403528
CTAGCGG5600.017.14235529
TAGCGGC5700.016.84161230
CATCGTT5850.016.40977728
ATCGTTT5900.016.2707129
ACCGTCG5500.016.0015988
CCCTATA703.6982555E-415.9995322
GGCATCG6200.015.99953126
GCATCGT6550.015.63312927
GTATTAC1153.3468132E-715.3043951
GATATAA1153.3468132E-715.3043951
AAATTCG1052.1866836E-615.2376521
CGGCATC6450.015.13134125
TCGTTTA6050.015.07393930
TACCGTC5550.014.9924897
Produced by FastQC (version 0.11.5)