##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1547625
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21661384379291	32.0	32.0	32.0	32.0	32.0
2	31.268363460140538	32.0	32.0	32.0	32.0	32.0
3	31.353856392860028	32.0	32.0	32.0	32.0	32.0
4	31.458286083515063	32.0	32.0	32.0	32.0	32.0
5	31.37775559324772	32.0	32.0	32.0	32.0	32.0
6	34.89312785720055	36.0	36.0	36.0	36.0	36.0
7	34.919802277683544	36.0	36.0	36.0	36.0	36.0
8	34.8553777562394	36.0	36.0	36.0	32.0	36.0
9	34.98235231402956	36.0	36.0	36.0	36.0	36.0
10	34.80577917777239	36.0	36.0	36.0	32.0	36.0
11	34.99146627897585	36.0	36.0	36.0	36.0	36.0
12	34.87081043534448	36.0	36.0	36.0	32.0	36.0
13	34.937064857442856	36.0	36.0	36.0	36.0	36.0
14	34.878665051288266	36.0	36.0	36.0	32.0	36.0
15	34.84845101364995	36.0	36.0	36.0	32.0	36.0
16	34.860571520878764	36.0	36.0	36.0	32.0	36.0
17	34.816602213068414	36.0	36.0	36.0	32.0	36.0
18	34.82204733058719	36.0	36.0	36.0	32.0	36.0
19	34.81682319683386	36.0	36.0	36.0	32.0	36.0
20	34.7967828123738	36.0	36.0	36.0	32.0	36.0
21	34.79235538324853	36.0	36.0	36.0	32.0	36.0
22	34.768421936838706	36.0	36.0	36.0	32.0	36.0
23	34.69983813908408	36.0	36.0	36.0	32.0	36.0
24	34.680611259187465	36.0	36.0	36.0	32.0	36.0
25	34.661825700670384	36.0	36.0	36.0	32.0	36.0
26	34.59384540828689	36.0	36.0	36.0	32.0	36.0
27	34.582205637670626	36.0	36.0	36.0	32.0	36.0
28	34.54960794766174	36.0	36.0	36.0	32.0	36.0
29	34.50932428721428	36.0	36.0	36.0	32.0	36.0
30	34.48549874808174	36.0	36.0	36.0	32.0	36.0
31	34.485028996042324	36.0	36.0	36.0	32.0	36.0
32	34.44173879331233	36.0	36.0	36.0	32.0	36.0
33	34.414678620466844	36.0	36.0	36.0	32.0	36.0
34	34.409974638559085	36.0	36.0	36.0	32.0	36.0
35	34.363520878765854	36.0	36.0	36.0	32.0	36.0
36	34.34027235279864	36.0	36.0	36.0	32.0	36.0
37	34.33711331879493	36.0	36.0	36.0	32.0	36.0
38	33.86950844035215	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	7.0
21	32.0
22	114.0
23	371.0
24	973.0
25	2473.0
26	5282.0
27	10684.0
28	18700.0
29	29598.0
30	44445.0
31	64948.0
32	93905.0
33	149709.0
34	360969.0
35	765412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54209052289567	18.474660387283215	12.367511945088136	26.615737144732982
2	15.976286245052904	20.862288991196188	36.1227041434456	27.038720620305305
3	17.243841369840883	24.986479282771988	28.916307244972135	28.853372102414994
4	11.967506319364393	16.184809747070346	35.98386946700232	35.863814466562935
5	14.543141320592934	36.69556474671157	32.57304912113609	16.188244811559404
6	34.35722477990469	35.849511348033275	16.38342621759147	13.40983765447056
7	30.321972425994286	30.573326604709045	20.988913923793806	18.11578704550286
8	28.609543428505702	31.91251103336956	19.454528301642952	20.02341723648178
9	28.00935409205988	13.737514199790896	18.332172364460654	39.92095934368857
10	16.32534378905225	25.79338668886009	30.36128058433477	27.519988937752892
11	38.21869866899637	20.59850577104256	22.054093104344016	19.12870245561706
12	25.364480655047483	23.454636268886343	27.7612684824953	23.419614593570873
13	29.429951977999824	18.69426940910712	25.224925434084767	26.650853178808287
14	24.439447540586382	19.596995396171554	24.26576205476133	31.69779500848074
15	25.796300783458527	26.552556336321782	21.79739924077215	25.85374363944754
16	26.648203956516568	24.960132435268516	23.013277126743965	25.378386481470947
17	24.542637912931102	25.32583797754624	24.64162830142961	25.489895808093046
18	26.032921411840725	23.60604151522494	25.655827477586623	24.70520959534771
19	26.3097972700105	24.25212826104515	24.92554720943381	24.51252725951054
20	26.458476698166542	23.115483401986918	24.227509894192714	26.198530005653826
21	28.286927759538337	23.522653772914982	23.399561779580687	24.790856687966
22	26.521799531540264	23.348873273564333	24.29393425409902	25.835392940796382
23	24.426620695091763	22.994747428798874	25.071609817119544	27.507022058989815
24	25.300250383652372	24.46742589451579	24.489007349971732	25.743316371860107
25	25.48713382578714	23.541893896708764	24.64164422366156	26.329328053842538
26	25.33154047753201	24.56126294242012	25.1806640372597	24.92653254278817
27	26.266586716153277	23.84907445219252	24.03781538668307	25.84652344497113
28	25.04159599386156	23.617478394313867	25.397625393748484	25.943300218076082
29	24.995331556417092	23.8210806881512	25.369130118730315	25.8144576367014
30	24.96224860673613	24.121347225587595	25.654664405136902	25.261739762539374
31	25.82227606816897	23.993990792343105	23.84531136418706	26.338421775300862
32	25.376754704789594	24.138987157741703	23.771520878765852	26.71273725870285
33	24.94842096761166	23.456199014619177	24.777061626686052	26.818318391083114
34	25.919004926904126	23.66419513771101	25.023309910346498	25.393490025038368
35	27.164138599466924	23.370390113884177	24.652871335110248	24.812599951538648
36	25.356142476375094	24.507616509167274	24.105128826427592	26.031112188030047
37	26.63332525644132	24.205669978192393	24.200565382440836	24.96043938292545
38	25.522478328069347	23.644438183951657	24.822566721632644	26.01051676634635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	161.5
2	149.0
3	149.0
4	462.5
5	776.0
6	776.0
7	913.5
8	1051.0
9	1044.0
10	1037.0
11	1037.0
12	1276.5
13	1516.0
14	2043.0
15	2570.0
16	2570.0
17	4052.0
18	5534.0
19	5534.0
20	6410.5
21	7287.0
22	7811.5
23	8336.0
24	8336.0
25	9676.5
26	11017.0
27	11017.0
28	14510.5
29	18004.0
30	22733.0
31	27462.0
32	27462.0
33	38316.0
34	49170.0
35	49170.0
36	54302.0
37	59434.0
38	69921.0
39	80408.0
40	80408.0
41	85018.5
42	89629.0
43	104994.5
44	120360.0
45	120360.0
46	122647.0
47	124934.0
48	124934.0
49	135308.0
50	145682.0
51	147129.0
52	148576.0
53	148576.0
54	141690.5
55	134805.0
56	134805.0
57	132439.5
58	130074.0
59	116762.5
60	103451.0
61	103451.0
62	101757.0
63	100063.0
64	81967.5
65	63872.0
66	63872.0
67	53897.5
68	43923.0
69	43923.0
70	35618.0
71	27313.0
72	21277.0
73	15241.0
74	15241.0
75	11287.5
76	7334.0
77	7334.0
78	7646.0
79	7958.0
80	6175.0
81	4392.0
82	4392.0
83	4245.5
84	4099.0
85	4099.0
86	2565.0
87	1031.0
88	880.5
89	730.0
90	730.0
91	436.0
92	142.0
93	97.0
94	52.0
95	52.0
96	36.0
97	20.0
98	20.0
99	19.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008206122284145061
2	0.0
3	0.0
4	6.46151360956304E-5
5	1.292302721912608E-4
6	0.0
7	5.169210887650432E-4
8	0.003036911396494629
9	0.004329214118407237
10	0.0012276875858169777
11	0.004587674662789758
12	7.753816331475649E-4
13	6.46151360956304E-5
14	0.0
15	0.0
16	6.46151360956304E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.292302721912608E-4
22	0.0
23	1.292302721912608E-4
24	0.0
25	6.46151360956304E-5
26	6.46151360956304E-5
27	6.46151360956304E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.46151360956304E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1547625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.7102431879298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23711812632784	30.964482506202057
2	14.292492550753932	11.922866801072432
3	4.801309619846361	6.007913755930153
4	2.1453127526820435	3.579260665141405
5	1.132303492529011	2.3614327017963648
6	0.6781125596976224	1.6970543864266436
7	0.46829563583569	1.3672907398186989
8	0.31962774964311647	1.0665400933780103
9	0.2572592473221987	0.9657311191337417
>10	1.4022146329598706	10.570776988065916
>50	0.11080015070474175	3.260419519291782
>100	0.11986309264694461	11.100156935971187
>500	0.02192046192135371	6.181693379182101
>1k	0.013369927129336306	8.95438040858961
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3583	0.23151603263064374	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3166	0.20457152087876587	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3048	0.19694693481948147	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2885	0.1864146676358937	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2826	0.1826023746062515	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2691	0.1738793312333414	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2580	0.16670705112672643	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2576	0.1664485905823439	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2570	0.16606089976577013	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2529	0.16341167918584928	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2435	0.15733785639286002	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2427	0.156820935304095	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2267	0.1464825135287941	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2244	0.14499636539859462	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2183	0.14105484209676117	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2138	0.1381471609724578	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2130	0.13763023988369275	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2116	0.13672562797835394	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2000	0.1292302721912608	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1935	0.12503028834504482	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1932	0.12483644293675793	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1920	0.12406106130361037	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1896	0.12251029803731525	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1895	0.12244568290121963	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1893	0.12231645262902836	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1827	0.11805185364671675	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1816	0.1173410871496648	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1715	0.11081495840400614	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1683	0.10874727404894596	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1602	0.1035134480251999	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1585	0.10241499071157419	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1574	0.10170422421452226	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1570	0.10144576367013974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.292302721912608E-4
13	0.0	0.0	0.0	0.0	1.938454082868912E-4
14	0.0	0.0	0.0	0.0	1.938454082868912E-4
15	0.0	0.0	0.0	0.0	3.23075680478152E-4
16	0.0	0.0	0.0	0.0	3.23075680478152E-4
17	0.0	0.0	0.0	6.46151360956304E-5	3.23075680478152E-4
18	0.0	0.0	0.0	6.46151360956304E-5	3.23075680478152E-4
19	0.0	0.0	0.0	6.46151360956304E-5	3.23075680478152E-4
20	0.0	0.0	0.0	1.292302721912608E-4	3.876908165737824E-4
21	0.0	0.0	0.0	2.584605443825216E-4	3.876908165737824E-4
22	0.0	0.0	0.0	7.107664970519344E-4	3.876908165737824E-4
23	0.0	0.0	0.0	0.0013569178580082385	3.876908165737824E-4
24	0.0	0.0	0.0	0.0020030692189645424	3.876908165737824E-4
25	0.0	0.0	0.0	0.002261529763347064	3.876908165737824E-4
26	0.0	0.0	0.0	0.002584605443825216	3.876908165737824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACA	125	0.0	20.4794	2
ACTAGAC	75	1.5092119E-6	19.200058	3
GTATAAA	135	1.8189894E-12	18.96302	1
GTATTAT	130	1.2732926E-11	18.461596	1
TTATACT	90	4.3690125E-7	17.777832	4
TCTAGCG	550	0.0	17.454035	28
CTAGCGG	560	0.0	17.142355	29
TAGCGGC	570	0.0	16.841612	30
CATCGTT	585	0.0	16.409777	28
ATCGTTT	590	0.0	16.27071	29
ACCGTCG	550	0.0	16.001598	8
CCCTATA	70	3.6982555E-4	15.999532	2
GGCATCG	620	0.0	15.999531	26
GCATCGT	655	0.0	15.633129	27
GTATTAC	115	3.3468132E-7	15.304395	1
GATATAA	115	3.3468132E-7	15.304395	1
AAATTCG	105	2.1866836E-6	15.23765	21
CGGCATC	645	0.0	15.131341	25
TCGTTTA	605	0.0	15.073939	30
TACCGTC	555	0.0	14.992489	7
>>END_MODULE