FastQCFastQC Report
Thu 2 Feb 2017
SRR4062757_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062757_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418992
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA36610.1513440309021278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31760.13129435731908168No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC28840.11922321363609305No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25250.1043823212313228No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24310.10049640511419632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG6150.017.43012829
ACGAACG1850.017.2976115
TCTAGCG6200.017.28956228
TAGCGGC6200.017.0315130
GTATTAG4750.016.5205921
CGAACGA2050.015.61003816
CATCGTT5600.015.42789428
TAGGACG2600.015.3810774
ATCGTTT5750.014.74717829
CGAGCCG6550.014.65675415
CGTCGTA5150.014.60190410
ATACCGT5150.014.5985846
ACCGTCG5050.014.5730138
TAACGAA2200.014.54571813
CGGTCCA7450.014.38922510
CCGTCGT5150.014.2912259
TACCGTC5350.014.0548767
AGCGGCG7550.013.9861431
CGTTTAT6100.013.90102931
GTTCTAG2650.013.8996891
Produced by FastQC (version 0.11.5)