##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062757_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418992
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262186480980507	32.0	32.0	32.0	32.0	32.0
2	30.867276535019545	32.0	32.0	32.0	32.0	32.0
3	30.92260825996944	32.0	32.0	32.0	32.0	32.0
4	30.99018392785094	32.0	32.0	32.0	32.0	32.0
5	30.885384904125356	32.0	32.0	32.0	32.0	32.0
6	34.547034053853835	36.0	36.0	36.0	32.0	36.0
7	34.49181642601546	36.0	36.0	36.0	32.0	36.0
8	34.47061379285256	36.0	36.0	36.0	32.0	36.0
9	34.630318744336485	36.0	36.0	36.0	32.0	36.0
10	34.33841037919927	36.0	36.0	36.0	32.0	36.0
11	34.60295073319796	36.0	36.0	36.0	32.0	36.0
12	34.42512377056229	36.0	36.0	36.0	32.0	36.0
13	34.51111413349032	36.0	36.0	36.0	32.0	36.0
14	34.40934777791741	36.0	36.0	36.0	32.0	36.0
15	34.34761503965288	36.0	36.0	36.0	32.0	36.0
16	34.374910706608375	36.0	36.0	36.0	32.0	36.0
17	34.29958428965453	36.0	36.0	36.0	32.0	36.0
18	34.324372713923815	36.0	36.0	36.0	32.0	36.0
19	34.312101073505	36.0	36.0	36.0	32.0	36.0
20	34.297370557653764	36.0	36.0	36.0	32.0	36.0
21	34.284077830765874	36.0	36.0	36.0	32.0	36.0
22	34.25970652238618	36.0	36.0	36.0	32.0	36.0
23	34.1951403725188	36.0	36.0	36.0	32.0	36.0
24	34.1847901936013	36.0	36.0	36.0	32.0	36.0
25	34.15594966829158	36.0	36.0	36.0	32.0	36.0
26	34.106419533425495	36.0	36.0	36.0	32.0	36.0
27	34.10541167560703	36.0	36.0	36.0	32.0	36.0
28	34.08826197027522	36.0	36.0	36.0	32.0	36.0
29	34.06100764285289	36.0	36.0	36.0	32.0	36.0
30	34.02484712640637	36.0	36.0	36.0	32.0	36.0
31	34.04117748219093	36.0	36.0	36.0	32.0	36.0
32	33.998856135117435	36.0	36.0	36.0	32.0	36.0
33	33.96550960069318	36.0	36.0	36.0	32.0	36.0
34	33.97673783129502	36.0	36.0	36.0	32.0	36.0
35	33.926630596546	36.0	36.0	36.0	32.0	36.0
36	33.89223775853744	36.0	36.0	36.0	32.0	36.0
37	33.881982660546214	36.0	36.0	36.0	32.0	36.0
38	33.27816586412853	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	3.0
9	10.0
10	34.0
11	29.0
12	8.0
13	16.0
14	209.0
15	493.0
16	742.0
17	799.0
18	1044.0
19	1311.0
20	1848.0
21	2624.0
22	4108.0
23	6471.0
24	9890.0
25	15141.0
26	21982.0
27	32293.0
28	46155.0
29	62750.0
30	85442.0
31	115638.0
32	159611.0
33	239867.0
34	527400.0
35	1083069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67591070001378	16.82261480471888	11.447753614220716	26.053720881046623
2	16.633115259527408	19.239495004101034	37.975987753564056	26.151401982807503
3	19.145002315274194	23.61033935304624	28.087748891975924	29.156909439703643
4	12.503023024595535	15.17882869940697	36.01084774911273	36.30730052688477
5	14.588273423883747	36.46758308446852	33.92701233245032	15.017131159197413
6	34.79656351876365	35.23538375170423	16.754747650461805	13.21330507907032
7	30.53959274755466	30.020243977757666	20.895613088266966	18.544550186420704
8	28.121712205739186	33.470153804178196	19.174653832082463	19.23348015800015
9	27.338002503526265	14.50089872293703	18.159742834606867	40.001355938929834
10	15.397545166708488	26.93917237119897	31.99121283613816	25.67206962595438
11	37.73245757638895	21.09634153197935	22.022699236358463	19.14850165527323
12	24.76254889064082	23.251791399381293	29.06219679121128	22.923462918766607
13	29.712123223019226	19.37403638988704	25.05777009301512	25.856070294078613
14	23.515498450030968	19.515883771753934	25.09610304131294	31.872514736902165
15	25.301148026060076	27.0423732598085	22.28263835938624	25.37384035474518
16	26.003123138220573	25.56578456134461	23.740481022821566	24.69061127761325
17	24.298318445169603	25.800752209242518	25.16601363834084	24.734915707247044
18	25.11777334682189	24.846817132668082	26.06430104335093	23.971108477159103
19	25.69758077185764	25.15098470804386	25.02707967792134	24.124354842177155
20	25.594438321210433	24.569483363499334	25.039791833930174	24.79628648136006
21	26.607979818316895	24.470001368431888	24.24877843682082	24.673240376430392
22	25.555349300250167	24.514623615993596	24.75953915858033	25.170487925175905
23	24.42990157308592	24.29880074369023	25.258801140589622	26.012496542634228
24	24.85509340630035	25.173207774493545	24.85563090133369	25.116067917872414
25	25.070816668864936	24.45863649124751	25.05159042392423	25.418956415963322
26	24.571667916059027	25.282808540303137	25.50290282153178	24.642620722106056
27	25.358322914806212	24.8378513358867	24.71038975213828	25.093435997168807
28	24.628943579083668	24.67470937479458	25.351522239820213	25.344824806301542
29	24.504774473517962	24.993054108494825	25.37177057389602	25.130400844091195
30	24.871315420776146	25.012495354291353	25.438226234332234	24.677962990600268
31	25.026872429548757	24.89854624290361	24.657107798957846	25.417473528589785
32	24.745019666663083	24.988279379894443	24.601064819370155	25.66563613407232
33	24.423094732524987	24.607940902145227	25.21097517413824	25.757989191191548
34	25.035639156435224	24.924752593141406	25.306157718564776	24.733450531858594
35	25.630642457118874	24.661033943120103	25.29288577506455	24.41543782469647
36	24.677737253891816	25.354161351955117	24.860198106260604	25.107903287892462
37	25.56583955934282	24.975989674009437	24.827400588154795	24.63077017849295
38	24.816574618069637	25.027585639509333	24.978471005208714	25.177368737212312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	122.5
2	178.0
3	178.0
4	419.0
5	660.0
6	660.0
7	801.5
8	943.0
9	981.5
10	1020.0
11	1020.0
12	1344.0
13	1668.0
14	2417.0
15	3166.0
16	3166.0
17	4732.5
18	6299.0
19	6299.0
20	8252.5
21	10206.0
22	12378.0
23	14550.0
24	14550.0
25	18815.0
26	23080.0
27	23080.0
28	29828.5
29	36577.0
30	45492.5
31	54408.0
32	54408.0
33	68440.0
34	82472.0
35	82472.0
36	92942.5
37	103413.0
38	118082.0
39	132751.0
40	132751.0
41	143685.0
42	154619.0
43	173044.5
44	191470.0
45	191470.0
46	203647.5
47	215825.0
48	215825.0
49	224693.0
50	233561.0
51	233053.0
52	232545.0
53	232545.0
54	222371.0
55	212197.0
56	212197.0
57	205201.0
58	198205.0
59	177538.0
60	156871.0
61	156871.0
62	144150.0
63	131429.0
64	108963.5
65	86498.0
66	86498.0
67	70547.0
68	54596.0
69	54596.0
70	43114.5
71	31633.0
72	24908.5
73	18184.0
74	18184.0
75	13457.5
76	8731.0
77	8731.0
78	8224.0
79	7717.0
80	6036.0
81	4355.0
82	4355.0
83	4371.0
84	4387.0
85	4387.0
86	2901.0
87	1415.0
88	1204.0
89	993.0
90	993.0
91	726.0
92	459.0
93	429.0
94	399.0
95	399.0
96	363.5
97	328.0
98	328.0
99	722.5
100	1117.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12033938103143789
2	0.05411344890764418
3	0.011244353019770219
4	0.0023563533901724354
5	3.3071626528735935E-4
6	2.480371989655195E-4
7	4.133953316091992E-5
8	2.8937673212643943E-4
9	1.6535813264367968E-4
10	3.7205579844827927E-4
11	0.0023150138570115155
12	0.003017785920747154
13	0.014592855205804731
14	0.00967345075965526
15	0.023976929233333555
16	0.011203013486609298
17	0.021331199111034677
18	0.006242269507298908
19	0.008433264764827664
20	0.006366288106781667
21	0.006821022971551787
22	0.008226567099023063
23	0.01004550655810354
24	0.01508892960373577
25	0.017486622527069125
26	0.01996699451672432
27	0.009260055428046062
28	0.006407627639942588
29	0.012236501815632298
30	0.0038445765839655527
31	0.006448967173103508
32	0.007358436902643745
33	0.009260055428046062
34	0.012567218080919656
35	0.015998399333276007
36	0.01616375746591969
37	0.009962827491781701
38	0.006655664838908107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2418992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.67716547104521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48224886464695	40.815452445117124
2	12.91337624654444	13.604801146581721
3	4.213994247544339	6.659438168157774
4	1.845861543198309	3.8893901579078474
5	0.9393678385649162	2.474161753513109
6	0.5561616530825777	1.7578211656848592
7	0.3851309992544873	1.4201326563030332
8	0.25348047126184436	1.0682106182670956
9	0.19727887305852682	0.9352882656040806
>10	1.0534261763000798	10.085671934718796
>50	0.07827661126529609	2.865252101172018
>100	0.0673144016022924	7.30157192927482
>500	0.009991191937664158	3.601560689727351
>1k	0.004090881738255563	3.521246967970452
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3661	0.1513440309021278	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3176	0.13129435731908168	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2884	0.11922321363609305	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2525	0.1043823212313228	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2431	0.10049640511419632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.133953316091992E-5
7	0.0	0.0	0.0	0.0	4.133953316091992E-5
8	0.0	0.0	0.0	0.0	4.133953316091992E-5
9	0.0	0.0	0.0	0.0	4.133953316091992E-5
10	0.0	0.0	0.0	4.133953316091992E-5	4.133953316091992E-5
11	0.0	0.0	0.0	4.133953316091992E-5	4.133953316091992E-5
12	0.0	0.0	0.0	4.133953316091992E-5	2.8937673212643943E-4
13	0.0	0.0	0.0	4.133953316091992E-5	3.7205579844827927E-4
14	0.0	0.0	0.0	4.133953316091992E-5	3.7205579844827927E-4
15	0.0	0.0	0.0	8.267906632183984E-5	4.133953316091992E-4
16	0.0	0.0	0.0	8.267906632183984E-5	4.133953316091992E-4
17	0.0	0.0	0.0	8.267906632183984E-5	4.133953316091992E-4
18	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4	4.133953316091992E-4
19	0.0	0.0	4.133953316091992E-5	1.6535813264367968E-4	4.133953316091992E-4
20	0.0	0.0	4.133953316091992E-5	2.066976658045996E-4	4.96074397931039E-4
21	0.0	0.0	4.133953316091992E-5	2.066976658045996E-4	4.96074397931039E-4
22	0.0	0.0	4.133953316091992E-5	2.066976658045996E-4	5.37413931091959E-4
23	0.0	0.0	4.133953316091992E-5	3.7205579844827927E-4	5.787534642528789E-4
24	0.0	0.0	4.133953316091992E-5	4.133953316091992E-4	5.787534642528789E-4
25	0.0	0.0	4.133953316091992E-5	6.614325305747187E-4	6.200929974137988E-4
26	0.0	0.0	4.133953316091992E-5	7.854511300574785E-4	6.200929974137988E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	615	0.0	17.430128	29
ACGAACG	185	0.0	17.29761	15
TCTAGCG	620	0.0	17.289562	28
TAGCGGC	620	0.0	17.03151	30
GTATTAG	475	0.0	16.520592	1
CGAACGA	205	0.0	15.610038	16
CATCGTT	560	0.0	15.427894	28
TAGGACG	260	0.0	15.381077	4
ATCGTTT	575	0.0	14.747178	29
CGAGCCG	655	0.0	14.656754	15
CGTCGTA	515	0.0	14.601904	10
ATACCGT	515	0.0	14.598584	6
ACCGTCG	505	0.0	14.573013	8
TAACGAA	220	0.0	14.545718	13
CGGTCCA	745	0.0	14.389225	10
CCGTCGT	515	0.0	14.291225	9
TACCGTC	535	0.0	14.054876	7
AGCGGCG	755	0.0	13.98614	31
CGTTTAT	610	0.0	13.901029	31
GTTCTAG	265	0.0	13.899689	1
>>END_MODULE