FastQCFastQC Report
Thu 2 Feb 2017
SRR4062757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418992
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA36500.1508892960373577No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC33380.1379913616911507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30640.12666432960505863No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27090.11198879533293206No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24440.10103381904528828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5400.019.85145629
TCTAGCG5400.019.25887528
TAGCGGC5800.018.20653230
AGTATCG450.00884982717.778895
ACGTATG2300.016.69531832
CATCGTT4900.016.65272728
GATATAC4950.016.485541
TCGCGTA2100.016.0010039
GTATTAG4700.016.0006711
GTTATTC2400.016.000013
CAACGTA2500.015.999680530
AACGTAT2450.015.67315631
TAGTACT2150.015.6279164
ATCGTTT5300.015.39591729
GCGTAAC2300.015.30499211
ACCGTCG4650.015.1407348
AGCGGCG7000.015.08541231
CGGCATC5450.014.97217725
CGCCGGT6700.014.8068977
CGTGTCG650.00416161214.7701569
Produced by FastQC (version 0.11.5)