##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418992
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17870129376203	32.0	32.0	32.0	32.0	32.0
2	31.19325404962067	32.0	32.0	32.0	32.0	32.0
3	31.32535700820838	32.0	32.0	32.0	32.0	32.0
4	31.438978715101165	32.0	32.0	32.0	32.0	32.0
5	31.34464479419527	32.0	32.0	32.0	32.0	32.0
6	34.87548491272398	36.0	36.0	36.0	36.0	36.0
7	34.91292075376851	36.0	36.0	36.0	36.0	36.0
8	34.83598457539339	36.0	36.0	36.0	32.0	36.0
9	34.9738812695536	36.0	36.0	36.0	36.0	36.0
10	34.79647307638884	36.0	36.0	36.0	32.0	36.0
11	34.99942083314041	36.0	36.0	36.0	36.0	36.0
12	34.891860328599684	36.0	36.0	36.0	32.0	36.0
13	34.95175345763855	36.0	36.0	36.0	36.0	36.0
14	34.89408315529774	36.0	36.0	36.0	32.0	36.0
15	34.85831867157891	36.0	36.0	36.0	32.0	36.0
16	34.8761599873005	36.0	36.0	36.0	32.0	36.0
17	34.84384735460059	36.0	36.0	36.0	32.0	36.0
18	34.845674561966305	36.0	36.0	36.0	32.0	36.0
19	34.84292134905779	36.0	36.0	36.0	32.0	36.0
20	34.82838347543109	36.0	36.0	36.0	32.0	36.0
21	34.82097584448398	36.0	36.0	36.0	32.0	36.0
22	34.799694666208076	36.0	36.0	36.0	32.0	36.0
23	34.73693712091648	36.0	36.0	36.0	32.0	36.0
24	34.71877914437088	36.0	36.0	36.0	32.0	36.0
25	34.697761712316534	36.0	36.0	36.0	32.0	36.0
26	34.63419184519833	36.0	36.0	36.0	32.0	36.0
27	34.617138460978786	36.0	36.0	36.0	32.0	36.0
28	34.58214289257674	36.0	36.0	36.0	32.0	36.0
29	34.54759420452817	36.0	36.0	36.0	32.0	36.0
30	34.532984813509096	36.0	36.0	36.0	32.0	36.0
31	34.526435804665745	36.0	36.0	36.0	32.0	36.0
32	34.49962629062023	36.0	36.0	36.0	32.0	36.0
33	34.47341413282888	36.0	36.0	36.0	32.0	36.0
34	34.46455879143048	36.0	36.0	36.0	32.0	36.0
35	34.43450495082249	36.0	36.0	36.0	32.0	36.0
36	34.40526549901777	36.0	36.0	36.0	32.0	36.0
37	34.39375367921845	36.0	36.0	36.0	32.0	36.0
38	33.94867531599939	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	13.0
21	40.0
22	142.0
23	540.0
24	1327.0
25	3653.0
26	7825.0
27	15378.0
28	27219.0
29	43897.0
30	67100.0
31	99588.0
32	146036.0
33	239043.0
34	576047.0
35	1191142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.034169005664445	18.002820440628735	12.141620682569783	26.821389871137036
2	15.59174234557204	20.17497370805691	37.223521202219764	27.009762744151285
3	18.11948944022965	24.409258071130456	28.352181404485837	29.119071084154065
4	12.065738179984209	16.07604826807883	35.886465012257176	35.97174853967978
5	14.177522777058698	36.96499904298941	33.5033904632138	15.354087716738093
6	35.02926222483856	35.59309281687515	16.3180568411018	13.059588117184493
7	30.445311660286567	30.161803735458747	20.766810804553987	18.626073799700702
8	28.1306429955749	33.66239094342618	18.90411785716648	19.302848203832443
9	27.25689362933565	14.297862664847658	17.904047294224647	40.54119641159205
10	15.194613903692028	27.166615198458516	31.96312147861341	25.675649419236045
11	37.95381952971196	20.978742861300663	21.825464744106515	19.241972864880864
12	24.634991083025835	23.389715177706986	28.848581758772905	23.126711980494274
13	29.75956464420851	19.370835808542996	24.935851246823567	25.93374830042493
14	23.4599880694058	19.454764403836144	24.940274684775595	32.14497284198246
15	25.274990574586436	27.16780377942548	22.055509071547156	25.501696574440924
16	26.024310952661274	25.607773816531843	23.580979184718263	24.78693604608862
17	24.354565868758556	25.840970123092593	25.04448133768115	24.759982670467696
18	25.167755825567014	24.859941661650804	25.9095110690734	24.062791443708782
19	25.726046220905236	25.26296077043661	24.898635464689427	24.112357543968727
20	25.692313161845924	24.465397157162982	24.981231851944944	24.86105782904615
21	26.608780272435904	24.55312979667969	24.127043052247817	24.711046878636587
22	25.56861564893433	24.54161635294745	24.69188574234939	25.197882255768832
23	24.43719163202748	24.266417526359195	25.141701994844944	26.154688846768376
24	24.931872449350802	25.163745890850404	24.75874248447287	25.145639175325922
25	25.15890916547058	24.449564115962353	24.978627461355803	25.41289925721127
26	24.705962113955778	25.190420303341355	25.348006668896243	24.755610913806624
27	25.460151104511475	24.8229838262943	24.540386310308275	25.176478758885946
28	24.703058133305113	24.69454218947396	25.256015728865577	25.34638394835535
29	24.68681169677287	24.915295296553275	25.184829052762474	25.21306395391138
30	24.916452803481782	24.990326549240343	25.33344467447598	24.759775972801894
31	25.05006217465787	24.820007672617354	24.655269632971088	25.474660519753684
32	24.86878832174724	24.90963178051023	24.536129098401318	25.685450799341215
33	24.42967980051195	24.57759265016172	25.202481033422185	25.790246515904148
34	25.07498991315391	24.795451989919766	25.32335782838472	24.806200268541605
35	25.770486219053225	24.59913054693856	25.14882231937931	24.481560914628904
36	24.811822445051494	25.221331860543568	24.856882536196895	25.10996315820805
37	25.704839040393683	24.886977716338045	24.801156845495974	24.607026397772295
38	25.003110801156346	24.787938442102515	24.92510306983366	25.283847686907475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	56.0
2	87.0
3	87.0
4	281.5
5	476.0
6	476.0
7	597.5
8	719.0
9	804.0
10	889.0
11	889.0
12	1162.0
13	1435.0
14	2164.0
15	2893.0
16	2893.0
17	4519.0
18	6145.0
19	6145.0
20	7935.0
21	9725.0
22	12044.5
23	14364.0
24	14364.0
25	18694.0
26	23024.0
27	23024.0
28	29560.5
29	36097.0
30	45087.0
31	54077.0
32	54077.0
33	68416.0
34	82755.0
35	82755.0
36	93167.5
37	103580.0
38	118148.5
39	132717.0
40	132717.0
41	143221.5
42	153726.0
43	171962.0
44	190198.0
45	190198.0
46	202828.5
47	215459.0
48	215459.0
49	225240.5
50	235022.0
51	233511.5
52	232001.0
53	232001.0
54	222193.5
55	212386.0
56	212386.0
57	206267.0
58	200148.0
59	179825.0
60	159502.0
61	159502.0
62	146113.0
63	132724.0
64	109723.0
65	86722.0
66	86722.0
67	71114.5
68	55507.0
69	55507.0
70	43929.0
71	32351.0
72	25336.5
73	18322.0
74	18322.0
75	13433.0
76	8544.0
77	8544.0
78	8069.0
79	7594.0
80	5876.5
81	4159.0
82	4159.0
83	3964.5
84	3770.0
85	3770.0
86	2380.5
87	991.0
88	796.0
89	601.0
90	601.0
91	377.5
92	154.0
93	106.0
94	58.0
95	58.0
96	36.5
97	15.0
98	15.0
99	22.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009136036828563301
2	0.0
3	0.0
4	8.267906632183984E-5
5	4.133953316091992E-5
6	1.2401859948275976E-4
7	4.96074397931039E-4
8	0.002645730122298875
9	0.0038032370508046327
10	9.508092627011582E-4
11	0.0055394974435632694
12	4.1339533160919924E-4
13	2.0669766580459962E-4
14	4.133953316091992E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.133953316091992E-5
22	1.2401859948275976E-4
23	2.8937673212643943E-4
24	0.0
25	0.0
26	4.133953316091992E-5
27	1.6535813264367968E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.133953316091992E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2418992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.43944288202235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36076333423615	38.01154917742808
2	14.195472425673556	14.32023441196169
3	4.5530043660364905	6.889530109868878
4	1.9834881657738619	4.001841521788719
5	1.065257437359745	2.6865495833178175
6	0.6372274900064681	1.9284839751021423
7	0.4078471162788316	1.4400106928300556
8	0.2766670212094857	1.1163944330908095
9	0.21661442971766362	0.9833320039649344
>10	1.1309108994575732	10.372864315092352
>50	0.0844024940767993	2.9572685759744264
>100	0.07340815651068817	7.7675031917800315
>500	0.009737714432020914	3.413720864152602
>1k	0.005198949230654859	4.110717143647527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3650	0.1508892960373577	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3338	0.1379913616911507	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3064	0.12666432960505863	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2709	0.11198879533293206	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2444	0.10103381904528828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2401859948275976E-4
2	0.0	0.0	0.0	0.0	1.2401859948275976E-4
3	0.0	0.0	0.0	0.0	1.2401859948275976E-4
4	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
5	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
6	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
7	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
8	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
9	0.0	0.0	0.0	4.133953316091992E-5	1.2401859948275976E-4
10	0.0	0.0	0.0	2.066976658045996E-4	1.2401859948275976E-4
11	0.0	0.0	0.0	2.066976658045996E-4	1.2401859948275976E-4
12	0.0	0.0	0.0	2.066976658045996E-4	3.7205579844827927E-4
13	0.0	0.0	0.0	2.066976658045996E-4	4.96074397931039E-4
14	0.0	0.0	0.0	2.066976658045996E-4	4.96074397931039E-4
15	0.0	0.0	0.0	2.8937673212643943E-4	5.37413931091959E-4
16	0.0	0.0	0.0	2.8937673212643943E-4	5.37413931091959E-4
17	0.0	0.0	0.0	2.8937673212643943E-4	5.37413931091959E-4
18	0.0	0.0	0.0	2.8937673212643943E-4	5.787534642528789E-4
19	0.0	0.0	0.0	3.3071626528735935E-4	6.200929974137988E-4
20	0.0	0.0	0.0	3.3071626528735935E-4	6.200929974137988E-4
21	0.0	0.0	0.0	3.3071626528735935E-4	6.200929974137988E-4
22	0.0	0.0	0.0	3.3071626528735935E-4	6.200929974137988E-4
23	0.0	0.0	0.0	4.547348647701191E-4	6.200929974137988E-4
24	0.0	0.0	0.0	5.37413931091959E-4	6.614325305747187E-4
25	0.0	0.0	0.0	8.267906632183984E-4	6.614325305747187E-4
26	0.0	0.0	0.0	0.001074827862183918	7.027720637356387E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	540	0.0	19.851456	29
TCTAGCG	540	0.0	19.258875	28
TAGCGGC	580	0.0	18.206532	30
AGTATCG	45	0.008849827	17.77889	5
ACGTATG	230	0.0	16.695318	32
CATCGTT	490	0.0	16.652727	28
GATATAC	495	0.0	16.48554	1
TCGCGTA	210	0.0	16.001003	9
GTATTAG	470	0.0	16.000671	1
GTTATTC	240	0.0	16.00001	3
CAACGTA	250	0.0	15.9996805	30
AACGTAT	245	0.0	15.673156	31
TAGTACT	215	0.0	15.627916	4
ATCGTTT	530	0.0	15.395917	29
GCGTAAC	230	0.0	15.304992	11
ACCGTCG	465	0.0	15.140734	8
AGCGGCG	700	0.0	15.085412	31
CGGCATC	545	0.0	14.972177	25
CGCCGGT	670	0.0	14.806897	7
CGTGTCG	65	0.004161612	14.770156	9
>>END_MODULE