FastQCFastQC Report
Thu 2 Feb 2017
SRR4062756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697916
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT7100.10173144045988343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3150.025.4256461
CGAGTAT350.002064171522.86011315
TATCGGC400.004482092319.99973125
GTATCAA8750.018.8556581
CGAGAAC450.00884756917.77753821
ACAATTC550.001364695117.453068
TGATCTA652.1014297E-417.22806718
TAACCTA652.1060382E-417.223135
GATCTAC753.231121E-517.06643719
ACTTTGC908.578048E-615.9986398
GTAGGAC3800.015.9951993
TTAACCT1304.816684E-915.9929074
TAGGACG4150.015.414854
TAGGACA1603.0559022E-1014.9933494
GGACGTG4600.014.9488216
ACTATAC756.25422E-414.9288523
TAAGAGT756.2610244E-414.9267134
GACGTGG2150.014.8824557
ATTATAC650.00416770714.7647983
GTAGACT650.004171679714.7626834
Produced by FastQC (version 0.11.5)