##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	697916
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292281592627194	32.0	32.0	32.0	32.0	32.0
2	31.396580677330796	32.0	32.0	32.0	32.0	32.0
3	31.43342465282355	32.0	32.0	32.0	32.0	32.0
4	31.512813862986377	32.0	32.0	32.0	32.0	32.0
5	31.475199021085633	32.0	32.0	32.0	32.0	32.0
6	35.00124513551774	36.0	36.0	36.0	36.0	36.0
7	35.02672957777154	36.0	36.0	36.0	36.0	36.0
8	34.95943924483749	36.0	36.0	36.0	36.0	36.0
9	35.04570177499871	36.0	36.0	36.0	36.0	36.0
10	34.939888181385726	36.0	36.0	36.0	32.0	36.0
11	35.0726720694181	36.0	36.0	36.0	36.0	36.0
12	35.00340442116243	36.0	36.0	36.0	36.0	36.0
13	35.03756755827349	36.0	36.0	36.0	36.0	36.0
14	34.99645659363018	36.0	36.0	36.0	36.0	36.0
15	34.97544833475662	36.0	36.0	36.0	36.0	36.0
16	34.991676648765754	36.0	36.0	36.0	36.0	36.0
17	34.98370864115452	36.0	36.0	36.0	36.0	36.0
18	34.97080880793677	36.0	36.0	36.0	36.0	36.0
19	34.960366290499145	36.0	36.0	36.0	36.0	36.0
20	34.95473953885568	36.0	36.0	36.0	36.0	36.0
21	34.933785727795325	36.0	36.0	36.0	36.0	36.0
22	34.93587193874335	36.0	36.0	36.0	36.0	36.0
23	34.886758578396254	36.0	36.0	36.0	32.0	36.0
24	34.86428022856619	36.0	36.0	36.0	32.0	36.0
25	34.85532786180572	36.0	36.0	36.0	32.0	36.0
26	34.789271201691896	36.0	36.0	36.0	32.0	36.0
27	34.773368141724795	36.0	36.0	36.0	32.0	36.0
28	34.7567629915348	36.0	36.0	36.0	32.0	36.0
29	34.716742415992755	36.0	36.0	36.0	32.0	36.0
30	34.71763507356186	36.0	36.0	36.0	32.0	36.0
31	34.696162575438876	36.0	36.0	36.0	32.0	36.0
32	34.674390614343274	36.0	36.0	36.0	32.0	36.0
33	34.670705357091684	36.0	36.0	36.0	32.0	36.0
34	34.64723834960081	36.0	36.0	36.0	32.0	36.0
35	34.6361610852882	36.0	36.0	36.0	32.0	36.0
36	34.620898503544836	36.0	36.0	36.0	32.0	36.0
37	34.60134170874432	36.0	36.0	36.0	32.0	36.0
38	34.24286303795872	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	8.0
22	48.0
23	135.0
24	406.0
25	978.0
26	2179.0
27	4256.0
28	7391.0
29	11707.0
30	17705.0
31	25415.0
32	35840.0
33	55887.0
34	141069.0
35	394888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74431034927476	18.70126073660951	12.715842750406251	26.838586163709472
2	14.809661907736748	21.095948509562756	38.4245381965738	25.669851386126695
3	18.017211240321185	24.594650359069	29.27401005278572	28.114128347824092
4	12.310077430521725	17.547813777016145	36.01966425758974	34.12244453487239
5	13.791957175239355	37.38555180151136	34.054195789166	14.768295234083281
6	33.08912976508601	36.30198519877062	17.50012537343373	13.108759662709643
7	29.43647703940176	31.006873349543568	21.1440394433117	18.412610167742972
8	26.73603697966892	34.91331815439814	19.61206309813626	18.73858176779668
9	27.28640494292764	14.958603554162142	18.697752928128665	39.05723857478156
10	15.174953431723742	28.858289153173803	32.753116492334144	23.213640922768306
11	35.78857170696479	21.63632247285358	23.37099968189465	19.20410613828699
12	24.37141072226871	24.64236178773255	29.596281479613474	21.389946010385263
13	29.803300110615034	20.821273620321072	24.992692530333162	24.382733738730735
14	22.705597808332236	20.77986462554233	25.95828151238831	30.556256053737123
15	24.36353372039042	28.992744112471986	22.298528762773742	24.345193404363847
16	24.069802096527376	26.91398391783538	25.27066294511087	23.745551040526365
17	23.176141541388933	27.098246780414836	26.092824924489484	23.63278675370675
18	23.41041042188458	26.75064047822374	27.169172221298837	22.66977687859284
19	25.04026272502708	25.665839441995885	26.10944583588856	23.184451997088477
20	25.554364708646887	26.176072765203834	25.75797087328561	22.511591652863668
21	25.592658131720913	25.404526339167376	25.08743901477974	23.915376514331975
22	25.4148063663822	26.32408484688702	25.161910602422065	23.099198184308715
23	23.888582420495332	26.54191412994419	25.713589763796453	23.855913685764026
24	24.482172639687295	26.17177425363511	25.92102774545934	23.425025361218253
25	24.182027897380053	26.277268721835757	26.035692025533198	23.505011355251
26	23.84531067536878	26.414534721276944	26.177686394474968	23.562468208879306
27	24.427329975713373	26.242020876467762	25.712586776326628	23.61806237149223
28	23.73895998945432	26.205732495028055	25.745361906017344	24.309945609500282
29	24.27355154488506	26.045827864671395	25.371248115819096	24.309372474624453
30	23.6714733578253	26.285541526487428	25.61081849391617	24.432166621771103
31	24.473432332830885	25.711976799500224	25.559093071372484	24.255497796296403
32	24.142733509476784	26.27952361029121	25.907272508439412	23.670470371792593
33	23.789682425965303	26.145123481909	26.32866992589366	23.73652416623204
34	24.016500553075154	26.085804022260557	26.324801265481806	23.572894159182482
35	23.887401922294373	26.09210850589469	26.206878764779717	23.813610807031218
36	23.507413499618863	26.34887293026668	26.340132623410266	23.80358094670419
37	23.998590088205457	26.09325477564635	25.41523621753907	24.492918918609117
38	23.788174474218888	26.622477841109365	25.645566645747188	23.943781038924566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.5
2	17.0
3	17.0
4	46.0
5	75.0
6	75.0
7	92.5
8	110.0
9	122.0
10	134.0
11	134.0
12	238.0
13	342.0
14	504.5
15	667.0
16	667.0
17	1024.5
18	1382.0
19	1382.0
20	1984.5
21	2587.0
22	3149.5
23	3712.0
24	3712.0
25	5512.5
26	7313.0
27	7313.0
28	10082.0
29	12851.0
30	16811.5
31	20772.0
32	20772.0
33	26368.5
34	31965.0
35	31965.0
36	36229.5
37	40494.0
38	45100.0
39	49706.0
40	49706.0
41	52734.0
42	55762.0
43	59274.5
44	62787.0
45	62787.0
46	64341.0
47	65895.0
48	65895.0
49	67723.5
50	69552.0
51	67867.0
52	66182.0
53	66182.0
54	61257.5
55	56333.0
56	56333.0
57	51475.5
58	46618.0
59	40901.0
60	35184.0
61	35184.0
62	30286.0
63	25388.0
64	20771.5
65	16155.0
66	16155.0
67	13587.0
68	11019.0
69	11019.0
70	8821.5
71	6624.0
72	5084.5
73	3545.0
74	3545.0
75	2780.5
76	2016.0
77	2016.0
78	1697.5
79	1379.0
80	1097.0
81	815.0
82	815.0
83	560.0
84	305.0
85	305.0
86	237.5
87	170.0
88	107.0
89	44.0
90	44.0
91	24.5
92	5.0
93	5.0
94	5.0
95	5.0
96	4.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01002986032703076
2	0.0
3	0.0
4	0.0
5	2.865674379151646E-4
6	1.432837189575823E-4
7	4.2985115687274686E-4
8	0.0027223906601940634
9	0.004298511568727468
10	0.0022925395033213166
11	0.004871646444557798
12	5.731348758303292E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.432837189575823E-4
22	0.0
23	1.432837189575823E-4
24	0.0
25	1.432837189575823E-4
26	1.432837189575823E-4
27	1.432837189575823E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.865674379151646E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	697916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.72128355336135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.568923021723876	15.842635066918431
2	15.659397800695027	9.621536002200699
3	8.701057464392097	8.019229607330427
4	5.7353744998092875	7.047922651734365
5	3.934823621927058	6.044141611084273
6	2.763190549593852	5.0933256231624675
7	2.0945082217205933	4.504218668902748
8	1.5654562659025344	3.84742606681424
9	1.2877330648014738	3.560473136232461
>10	6.553268300232619	33.21218212310003
>50	0.12081420939818871	2.3706558877606527
>100	0.014984707688086294	0.750657328184046
>500	4.6827211525269524E-4	0.08559622657522162
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.432837189575823E-4	0.0
21	0.0	0.0	0.0	1.432837189575823E-4	0.0
22	0.0	0.0	0.0	1.432837189575823E-4	0.0
23	0.0	0.0	0.0	2.865674379151646E-4	0.0
24	0.0	0.0	0.0	2.865674379151646E-4	0.0
25	0.0	0.0	0.0	4.2985115687274686E-4	0.0
26	0.0	0.0	0.0	5.731348758303292E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAC	35	0.0020648714	22.858782	3
CCGTGTG	35	0.0020665925	22.855507	32
GTATCAA	920	0.0	20.526152	1
TAGGCGC	40	0.0044761715	20.004301	6
GATCGAA	40	0.0044836034	19.998568	22
GGTATCA	320	0.0	19.504192	1
CTACTCT	85	2.3908433E-7	18.824877	4
TATTGAG	70	1.7823204E-5	18.289646	5
TAGGACC	265	0.0	18.114506	4
GATATAC	160	0.0	18.00387	1
TACCGGA	45	0.008835941	17.781599	7
TTTCCGT	180	0.0	17.776505	24
CTTAGAC	65	2.097765E-4	17.232004	3
TCGCCCA	65	2.1000636E-4	17.229536	26
GTAGGAC	495	0.0	16.48603	3
GTCCTAA	270	0.0	16.00344	1
GGACCGC	70	3.6877563E-4	16.00344	6
CAGATCG	70	3.689769E-4	16.002293	9
TAAAGGT	70	3.691783E-4	16.001146	4
TCAATGC	60	0.0024394486	16.001146	3
>>END_MODULE