FastQCFastQC Report
Thu 2 Feb 2017
SRR4062755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102161
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27800.25223175198541775No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23870.21657452949251518No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18890.17139056816563097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16770.1521556288055919No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11500.10434047294360804No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11260.10216293263869798No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11200.10161854756247045No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11030.10007612317982581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC805.2623363E-921.9962273
TAGACCG1102.3646862E-1120.3592195
GACCGGT1450.019.8613647
CTAGACC1550.018.5766144
AGACCGG1301.2732926E-1118.4575336
AATAGCG1401.8189894E-1218.2817485
TTAATAC753.2382588E-517.063743
TAGCGTA1354.4383341E-1016.5920057
ACCGGTC1551.0913936E-1116.5155438
GTGTAGG1651.8189894E-1216.4969881
GTCCTAG2450.016.3385541
ATAGCGT1601.8189894E-1115.996536
TTCGTTC2950.015.72897129
TTTCGTT2800.015.42802428
TCGTTCT2700.015.40756130
CTAGCGG2700.015.40756129
CGGTCCA3850.015.37677610
GTATAGG1153.3165634E-715.3156191
CTAGGAC2000.015.1973923
CGTTATT1603.0377123E-1015.0015122
Produced by FastQC (version 0.11.5)