##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062755_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1102161
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242131594204476	32.0	32.0	32.0	32.0	32.0
2	30.90585041568337	32.0	32.0	32.0	32.0	32.0
3	30.958807288590325	32.0	32.0	32.0	32.0	32.0
4	31.01478368405342	32.0	32.0	32.0	32.0	32.0
5	30.942894912812193	32.0	32.0	32.0	32.0	32.0
6	34.59203600925817	36.0	36.0	36.0	32.0	36.0
7	34.51490299511596	36.0	36.0	36.0	32.0	36.0
8	34.49847436082387	36.0	36.0	36.0	32.0	36.0
9	34.61548539641667	36.0	36.0	36.0	32.0	36.0
10	34.37012196947633	36.0	36.0	36.0	32.0	36.0
11	34.615078922226424	36.0	36.0	36.0	32.0	36.0
12	34.45334393069615	36.0	36.0	36.0	32.0	36.0
13	34.52602478222329	36.0	36.0	36.0	32.0	36.0
14	34.43288321760614	36.0	36.0	36.0	32.0	36.0
15	34.38481401537525	36.0	36.0	36.0	32.0	36.0
16	34.40171354275827	36.0	36.0	36.0	32.0	36.0
17	34.34275936092821	36.0	36.0	36.0	32.0	36.0
18	34.351250860808904	36.0	36.0	36.0	32.0	36.0
19	34.33752691303721	36.0	36.0	36.0	32.0	36.0
20	34.313735470589144	36.0	36.0	36.0	32.0	36.0
21	34.29783670443792	36.0	36.0	36.0	32.0	36.0
22	34.2606479452639	36.0	36.0	36.0	32.0	36.0
23	34.21096282666507	36.0	36.0	36.0	32.0	36.0
24	34.19775241548195	36.0	36.0	36.0	32.0	36.0
25	34.17129711539421	36.0	36.0	36.0	32.0	36.0
26	34.12991296189939	36.0	36.0	36.0	32.0	36.0
27	34.122073816801716	36.0	36.0	36.0	32.0	36.0
28	34.10496560847281	36.0	36.0	36.0	32.0	36.0
29	34.067729669258846	36.0	36.0	36.0	32.0	36.0
30	34.03476261635097	36.0	36.0	36.0	32.0	36.0
31	34.049184284328696	36.0	36.0	36.0	32.0	36.0
32	34.013283903168414	36.0	36.0	36.0	32.0	36.0
33	33.974966452269676	36.0	36.0	36.0	32.0	36.0
34	33.97006698658363	36.0	36.0	36.0	32.0	36.0
35	33.91644959311752	36.0	36.0	36.0	32.0	36.0
36	33.88890007902657	36.0	36.0	36.0	32.0	36.0
37	33.86614569014872	36.0	36.0	36.0	32.0	36.0
38	33.24603302058411	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	2.0
10	8.0
11	10.0
12	7.0
13	10.0
14	131.0
15	271.0
16	379.0
17	456.0
18	602.0
19	792.0
20	1015.0
21	1412.0
22	2133.0
23	3026.0
24	4356.0
25	6698.0
26	9688.0
27	14045.0
28	20023.0
29	28152.0
30	38482.0
31	52519.0
32	71138.0
33	106286.0
34	238810.0
35	501704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.915945349125565	17.060730561669356	11.359559043273602	25.66376504593148
2	16.547760512505345	19.814918538920395	37.89177073792018	25.745550210654077
3	18.775265758347057	24.477534742544474	28.241571274959508	28.50562822414896
4	12.487943854427996	15.681019112903591	35.95984158020569	35.871195452462715
5	14.468887400898964	36.65018990017765	33.715583428147326	15.16533927077606
6	33.639762756122764	35.61888188343403	17.39824725515512	13.343108105288085
7	30.275522360163354	30.447457313405213	21.201893371295117	18.07512695513632
8	28.11607431252926	32.76514395421338	19.682966839539954	19.435814893717403
9	27.781149940888856	14.355797383503862	18.434874759676674	39.42817791593061
10	15.91816403485532	26.604310097663124	31.536279800681573	25.941246066799984
11	37.317548299174064	21.33964710504373	22.138365236389898	19.20443935939231
12	25.29402050930286	23.474550281544712	28.967802463221346	22.263626745931077
13	29.56481180683227	19.712596846115176	25.177176893773716	25.545414453278838
14	23.918337939883163	19.72035853052573	25.268634749295405	31.0926687802957
15	25.403734092264184	26.70330637688867	22.780267918805773	25.11269161204137
16	25.830741142693288	25.69989147281194	23.941496949260817	24.52787043523395
17	24.091062954718087	25.550244618706092	25.512220045212036	24.846472381363785
18	25.091507953070984	24.48520538250052	26.48631237013311	23.936974294295386
19	25.384983226881268	25.32491395666846	25.368468714526692	23.921634101923576
20	25.93624301441533	24.035220322096816	25.323680167971613	24.70485649551625
21	26.964571208730987	24.138506738965138	24.243942008786878	24.652980043517
22	25.754028745644597	24.561919279907084	24.806729094076655	24.87732288037166
23	24.252925190437953	24.218898159364464	25.461474595418597	26.066702054778983
24	24.694962041535618	25.34324143325644	25.06538148120589	24.896415044002055
25	25.192179424936363	24.40195469003099	24.927288979232554	25.478576905800093
26	24.372616742198886	25.2352630499529	25.676568435457614	24.715551772390597
27	25.341926913434172	24.96372711904855	24.746135202796935	24.948210764720347
28	24.527759176688573	24.68736559578835	25.504361728576537	25.280513498946544
29	24.4652683004733	25.193457998199715	25.69733804698162	24.643935654345363
30	24.68120820592852	25.107020043007633	25.843503035032168	24.368268716031686
31	25.03075934757518	24.9214230235985	24.543328034377883	25.504489594448437
32	24.570017212922124	24.95356503481148	24.635983445832828	25.84043430643357
33	24.1912389207082	24.661723179221383	25.477289720304597	25.669748179765822
34	24.93614017130314	24.941130992728826	25.69810248969395	24.42462634627408
35	25.630112932571674	24.62302118449888	25.5322894594754	24.21457642345404
36	24.423492259387647	25.308535545109713	25.08321385143106	25.18475834407158
37	25.387751472471077	25.146748041620278	24.842317879532764	24.623182606375885
38	24.592752154310027	25.36065468159221	25.129911903986326	24.916681260111442
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	319.0
1	274.0
2	229.0
3	229.0
4	531.0
5	833.0
6	833.0
7	949.0
8	1065.0
9	1012.0
10	959.0
11	959.0
12	1208.5
13	1458.0
14	1855.0
15	2252.0
16	2252.0
17	3253.0
18	4254.0
19	4254.0
20	5223.0
21	6192.0
22	6471.5
23	6751.0
24	6751.0
25	8131.5
26	9512.0
27	9512.0
28	12666.0
29	15820.0
30	19578.5
31	23337.0
32	23337.0
33	30052.0
34	36767.0
35	36767.0
36	41812.5
37	46858.0
38	54027.5
39	61197.0
40	61197.0
41	65902.0
42	70607.0
43	79830.0
44	89053.0
45	89053.0
46	95642.0
47	102231.0
48	102231.0
49	104659.0
50	107087.0
51	106244.0
52	105401.0
53	105401.0
54	99614.5
55	93828.0
56	93828.0
57	91241.0
58	88654.0
59	78737.0
60	68820.0
61	68820.0
62	63680.0
63	58540.0
64	48292.0
65	38044.0
66	38044.0
67	31331.5
68	24619.0
69	24619.0
70	19597.5
71	14576.0
72	11357.0
73	8138.0
74	8138.0
75	6145.0
76	4152.0
77	4152.0
78	3984.0
79	3816.0
80	2998.0
81	2180.0
82	2180.0
83	2228.5
84	2277.0
85	2277.0
86	1503.5
87	730.0
88	610.5
89	491.0
90	491.0
91	363.0
92	235.0
93	218.5
94	202.0
95	202.0
96	181.0
97	160.0
98	160.0
99	338.5
100	517.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11631694462061351
2	0.05371266085444867
3	0.011432086600777925
4	0.0023590019969859212
5	2.7219253811376013E-4
6	3.629233841516802E-4
7	0.0
8	4.5365423018960024E-4
9	0.0
10	4.5365423018960024E-4
11	0.001814616920758401
12	0.0024497328430238413
13	0.014063281135877608
14	0.009708200526057444
15	0.02359001996985921
16	0.012067202523043366
17	0.02195686474117665
18	0.006441890068692323
19	0.009073084603792005
20	0.006351159222654403
21	0.0057160433003889625
22	0.007349198529071525
23	0.008710161219640324
24	0.015152051288332647
25	0.01778324582343233
26	0.01841836174569777
27	0.008891622911716164
28	0.0068048134528440034
29	0.010252585602284967
30	0.0028126562271755216
31	0.005171658224161443
32	0.007439929375109444
33	0.009526738833981606
34	0.012520856753232968
35	0.015968628902673927
36	0.01642228313286353
37	0.009254546295867845
38	0.006169697530578564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1102161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.0470632789132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.26446882313725	47.98428232927054
2	12.061279377374099	14.243662532409186
3	3.2711635866322792	5.7945780988665865
4	1.2275607199766045	2.8993542204466727
5	0.5808513112097918	1.714878206432214
6	0.3396740099379034	1.2034051655403344
7	0.22196987023958117	0.917466828183409
8	0.14370396955490428	0.678823790699156
9	0.1118035735951311	0.5941505416392302
>10	0.6126911885118506	6.8112793101711535
>50	0.07433708717531518	3.0568796895177264
>100	0.08217142470080795	9.845060432090166
>500	0.00647504507570049	2.668316000212809
>1k	0.0018500128787715684	1.587862854520743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2780	0.25223175198541775	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2387	0.21657452949251518	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1889	0.17139056816563097	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1677	0.1521556288055919	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1150	0.10434047294360804	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1126	0.10216293263869798	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1120	0.10161854756247045	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1103	0.10007612317982581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.814616920758401E-4	0.0	9.073084603792005E-5	0.0
11	0.0	1.814616920758401E-4	0.0	9.073084603792005E-5	0.0
12	0.0	1.814616920758401E-4	0.0	9.073084603792005E-5	1.814616920758401E-4
13	0.0	1.814616920758401E-4	0.0	9.073084603792005E-5	2.7219253811376013E-4
14	0.0	1.814616920758401E-4	0.0	1.814616920758401E-4	2.7219253811376013E-4
15	0.0	1.814616920758401E-4	0.0	1.814616920758401E-4	2.7219253811376013E-4
16	0.0	1.814616920758401E-4	0.0	3.629233841516802E-4	3.629233841516802E-4
17	0.0	1.814616920758401E-4	0.0	4.5365423018960024E-4	3.629233841516802E-4
18	0.0	1.814616920758401E-4	0.0	4.5365423018960024E-4	3.629233841516802E-4
19	0.0	1.814616920758401E-4	0.0	5.443850762275203E-4	3.629233841516802E-4
20	0.0	2.7219253811376013E-4	0.0	5.443850762275203E-4	3.629233841516802E-4
21	0.0	2.7219253811376013E-4	0.0	6.351159222654403E-4	5.443850762275203E-4
22	0.0	2.7219253811376013E-4	0.0	9.073084603792005E-4	5.443850762275203E-4
23	0.0	2.7219253811376013E-4	0.0	0.002086809458872161	5.443850762275203E-4
24	0.0	2.7219253811376013E-4	0.0	0.0029033870732134417	5.443850762275203E-4
25	0.0	2.7219253811376013E-4	0.0	0.0033570413034030417	7.258467683033604E-4
26	0.0	2.7219253811376013E-4	0.0	0.004173618917744322	7.258467683033604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACC	80	5.2623363E-9	21.996227	3
TAGACCG	110	2.3646862E-11	20.359219	5
GACCGGT	145	0.0	19.861364	7
CTAGACC	155	0.0	18.576614	4
AGACCGG	130	1.2732926E-11	18.457533	6
AATAGCG	140	1.8189894E-12	18.281748	5
TTAATAC	75	3.2382588E-5	17.06374	3
TAGCGTA	135	4.4383341E-10	16.592005	7
ACCGGTC	155	1.0913936E-11	16.515543	8
GTGTAGG	165	1.8189894E-12	16.496988	1
GTCCTAG	245	0.0	16.338554	1
ATAGCGT	160	1.8189894E-11	15.99653	6
TTCGTTC	295	0.0	15.728971	29
TTTCGTT	280	0.0	15.428024	28
TCGTTCT	270	0.0	15.407561	30
CTAGCGG	270	0.0	15.407561	29
CGGTCCA	385	0.0	15.376776	10
GTATAGG	115	3.3165634E-7	15.315619	1
CTAGGAC	200	0.0	15.197392	3
CGTTATT	160	3.0377123E-10	15.001512	2
>>END_MODULE