FastQCFastQC Report
Thu 2 Feb 2017
SRR4062755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102161
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31510.2858928958654861No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23530.21348968072722588No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22470.20387221104720635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21230.19262158613850427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13500.12248664215119207No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12380.11232478739494503No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12270.11132674808852791No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12180.11051017047418663No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12170.1104194396281487No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11940.10833263016927652No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11240.10198147094662215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC1150.022.2623848
CCGTTAA400.00448395419.99954816
CGACGGA507.1967806E-419.19956424
CTAGCGG2550.018.82310329
TCTAGCG2650.018.11279728
GTCCTAG2150.017.8616811
CGGTCCA3700.017.73013110
TAGACCG1006.8081135E-817.6011965
TACGCTG550.001363974917.4557345
TAGTACT1151.8399078E-816.6960324
TAGCGGC3000.016.53295930
GTACCGC600.002440711716.001096
GACCGGT1407.421477E-1016.001097
GTATTAA1001.3133158E-616.0010871
ACTATAC1001.3139379E-616.0003623
GCGACGC908.582008E-615.99963832
AGGTCGT600.002442190215.99963728
GTCTAGG1753.6379788E-1215.5439141
CCGGTCC4550.015.4728799
CGTGATT1153.3452488E-715.30400228
Produced by FastQC (version 0.11.5)