FastQCFastQC Report
Thu 2 Feb 2017
SRR4062754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638205
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43810.26742684828821794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28930.1765957251992272No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20190.12324464886873132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19950.12177963075439276No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18300.11170763121831517No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18220.11121929184686899No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17700.10804508593246877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17280.1054813042323763No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17050.10407732853946851No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16580.10120833473222215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3750.017.06539529
CATCGTT3950.016.20132428
TCGTTTA3950.016.2008330
CGTAGTA703.6816712E-416.0090712
TAGGACG1800.015.9968584
TAACGCT703.7031324E-415.9968574
CGTTTAT4050.015.80081131
CTATACA1551.891749E-1015.4808284
TAAACGC4250.015.43414628
CGCTTCG3900.015.17789532
CCGATAA1807.2759576E-1215.1109099
TTAGTAC756.246992E-414.9331343
GTAAACG4200.014.85558327
GTATAGG1205.341135E-714.6857491
AAACGCT4200.014.47511229
GGCATCG4700.014.29680826
CCTATAC1351.3198587E-714.2220333
AACGCTT4200.014.09375930
TACCGTC3750.014.0776627
CTCGTAC800.001018372813.9998133
Produced by FastQC (version 0.11.5)