##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062754_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1638205
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2590426717047	32.0	32.0	32.0	32.0	32.0
2	30.918357592608984	32.0	32.0	32.0	32.0	32.0
3	30.963514944710827	32.0	32.0	32.0	32.0	32.0
4	31.02871801758632	32.0	32.0	32.0	32.0	32.0
5	30.956550004425576	32.0	32.0	32.0	32.0	32.0
6	34.60183249349135	36.0	36.0	36.0	32.0	36.0
7	34.53172160993282	36.0	36.0	36.0	32.0	36.0
8	34.52833802851291	36.0	36.0	36.0	32.0	36.0
9	34.63501637462955	36.0	36.0	36.0	32.0	36.0
10	34.39009220457757	36.0	36.0	36.0	32.0	36.0
11	34.63655525407382	36.0	36.0	36.0	32.0	36.0
12	34.473695904969155	36.0	36.0	36.0	32.0	36.0
13	34.54327144649174	36.0	36.0	36.0	32.0	36.0
14	34.44832850589517	36.0	36.0	36.0	32.0	36.0
15	34.400648270515596	36.0	36.0	36.0	32.0	36.0
16	34.41663528068831	36.0	36.0	36.0	32.0	36.0
17	34.35193580778962	36.0	36.0	36.0	32.0	36.0
18	34.36820300267671	36.0	36.0	36.0	32.0	36.0
19	34.351988304272055	36.0	36.0	36.0	32.0	36.0
20	34.3337982731099	36.0	36.0	36.0	32.0	36.0
21	34.303368015602445	36.0	36.0	36.0	32.0	36.0
22	34.28614489639575	36.0	36.0	36.0	32.0	36.0
23	34.234550010529816	36.0	36.0	36.0	32.0	36.0
24	34.214600736782025	36.0	36.0	36.0	32.0	36.0
25	34.1940740017275	36.0	36.0	36.0	32.0	36.0
26	34.146689822091865	36.0	36.0	36.0	32.0	36.0
27	34.153869021276336	36.0	36.0	36.0	32.0	36.0
28	34.12596897213719	36.0	36.0	36.0	32.0	36.0
29	34.10325813924387	36.0	36.0	36.0	32.0	36.0
30	34.067555647797434	36.0	36.0	36.0	32.0	36.0
31	34.092097142909466	36.0	36.0	36.0	32.0	36.0
32	34.04634096465339	36.0	36.0	36.0	32.0	36.0
33	33.997188996493115	36.0	36.0	36.0	32.0	36.0
34	34.00225429662344	36.0	36.0	36.0	32.0	36.0
35	33.951785643432906	36.0	36.0	36.0	32.0	36.0
36	33.91560091685717	36.0	36.0	36.0	32.0	36.0
37	33.91664779438471	36.0	36.0	36.0	32.0	36.0
38	33.315284106689944	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	10.0
11	10.0
12	9.0
13	14.0
14	158.0
15	338.0
16	553.0
17	636.0
18	860.0
19	1108.0
20	1503.0
21	1973.0
22	3062.0
23	4407.0
24	6615.0
25	9986.0
26	14572.0
27	20974.0
28	29954.0
29	40794.0
30	55668.0
31	76112.0
32	103510.0
33	156367.0
34	352453.0
35	756551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11060153987755	17.399895125024905	11.790245160533273	25.69925817456427
2	16.65347415122043	19.9953337584255	37.70005594603772	25.651136144316343
3	18.66517460044188	24.451998385833427	28.3740446411074	28.508782372617286
4	12.613153211997643	15.912535749059156	36.035478938195716	35.43883210074748
5	14.799185694098114	36.502879676231	33.27941252773615	15.41852210193474
6	33.62265512309555	35.54153066874049	17.379817714453495	13.45599649371047
7	30.23681407394069	30.51974569727232	21.05792620581673	18.185514022970263
8	28.171338856454998	32.294390686385825	19.79350557073478	19.7407648864244
9	27.963506354218616	14.61381770146923	18.590919440386813	38.831756503925334
10	15.984180207434864	26.41299816078735	31.47971463819153	26.12310699358626
11	37.03924589667583	21.303040439901743	22.483616004063038	19.174097659359386
12	25.41572277863544	23.69294780581514	28.488906225296383	22.40242319025303
13	29.562079891573106	19.590590792261153	25.078176034970117	25.769153281195628
14	24.135712253686386	20.022050555109907	25.13134487629834	30.71089231490537
15	25.44528920291218	26.766808400475757	22.645700483327758	25.142201913284307
16	25.91200213428798	25.618476487118265	23.844929570328024	24.624591808265723
17	24.11560603294639	25.57847891747407	25.330474340806642	24.975440708772904
18	24.964105193758698	24.59020096207848	26.379874490269334	24.065819353893488
19	25.652316178759843	24.92311148498919	25.57503085918566	23.849541477065305
20	25.850109241548047	24.10346538975028	25.169203833923543	24.877221534778126
21	27.357242580020525	24.101052981147188	24.264839195878668	24.276865242953622
22	25.86524549933154	24.3417638835473	24.816524122606207	24.976466494514955
23	24.379232919061273	24.148532343866563	25.3491643132644	26.123070423807764
24	24.776400056167088	25.148995402846207	25.152719524777623	24.92188501620909
25	24.950241770049818	24.42958142033799	25.209106671876526	25.411070137735663
26	24.632027918744537	25.294402086579364	25.714025798951827	24.35954419572427
27	25.43533097607908	24.745919573350005	24.81392668418328	25.004822766387637
28	24.422131780508664	24.741397676501236	25.61751701479672	25.218953528193378
29	24.51600338944145	24.86996559277375	25.626546066367606	24.98748495141719
30	24.467806513719857	25.003281094243402	25.93779777837872	24.591114613658018
31	25.20940175896867	24.876519959783142	24.567079681024396	25.34699860022379
32	24.569776485224974	25.097033029689236	24.636133769447813	25.697056715637977
33	24.378491530586892	24.504676537219694	25.35622692789262	25.76060500430079
34	25.085042112023597	24.711601624408125	25.587846731281576	24.6155095322867
35	25.773548343375424	24.7076356323404	25.278230752183077	24.240585272101097
36	24.627247473976617	25.292530297017613	24.928843981806526	25.15137824719924
37	25.507751571223185	25.073883355564984	24.87346273476776	24.544902338444068
38	24.540860024039794	25.12006163173937	25.33775060023185	25.001327743988984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	528.0
1	430.5
2	333.0
3	333.0
4	772.0
5	1211.0
6	1211.0
7	1403.5
8	1596.0
9	1526.5
10	1457.0
11	1457.0
12	1844.0
13	2231.0
14	2910.0
15	3589.0
16	3589.0
17	5172.0
18	6755.0
19	6755.0
20	8218.0
21	9681.0
22	10368.5
23	11056.0
24	11056.0
25	13511.0
26	15966.0
27	15966.0
28	20952.5
29	25939.0
30	32117.5
31	38296.0
32	38296.0
33	47986.0
34	57676.0
35	57676.0
36	64302.0
37	70928.0
38	81097.5
39	91267.0
40	91267.0
41	97602.5
42	103938.0
43	116443.5
44	128949.0
45	128949.0
46	133656.0
47	138363.0
48	138363.0
49	145487.5
50	152612.0
51	152459.0
52	152306.0
53	152306.0
54	145164.5
55	138023.0
56	138023.0
57	133847.5
58	129672.0
59	116473.0
60	103274.0
61	103274.0
62	96952.0
63	90630.0
64	75159.5
65	59689.0
66	59689.0
67	49322.0
68	38955.0
69	38955.0
70	31454.0
71	23953.0
72	18892.0
73	13831.0
74	13831.0
75	10430.0
76	7029.0
77	7029.0
78	6824.0
79	6619.0
80	5188.5
81	3758.0
82	3758.0
83	3912.5
84	4067.0
85	4067.0
86	2695.5
87	1324.0
88	1142.0
89	960.0
90	960.0
91	696.5
92	433.0
93	383.0
94	333.0
95	333.0
96	303.5
97	274.0
98	274.0
99	489.0
100	704.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12031461264005422
2	0.05573173076629604
3	0.01269682365760085
4	0.0030521210715386657
5	0.0
6	3.6625452858463986E-4
7	0.0
8	6.104242143077332E-5
9	1.2208484286154665E-4
10	2.441696857230933E-4
11	0.0012818908500462397
12	0.0020754423286462927
13	0.014344969036231729
14	0.009217405636046772
15	0.023989671622293915
16	0.011475975228985382
17	0.02026608391501674
18	0.005188605821615732
19	0.0072640481502620246
20	0.005615902771631145
21	0.005737987614492691
22	0.007019878464538932
23	0.009217405636046772
24	0.014344969036231729
25	0.017397090107770394
26	0.018862108222108955
27	0.008606981421739037
28	0.004395054343015679
29	0.010804508593246876
30	0.0019533574857847464
31	0.005249648243046505
32	0.006287369407369651
33	0.008179684471723623
34	0.01178118733613925
35	0.016237284100585704
36	0.015565817464847196
37	0.009156363214615998
38	0.005371733085908052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1638205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.66769365751585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97083449864427	43.96124222748913
2	13.145621958168865	14.63572912209716
3	3.8855003047774783	6.488905220176114
4	1.5180866859292468	3.3803353831145113
5	0.7617287903055752	2.120184247442044
6	0.4139179378158548	1.3825114177010218
7	0.23992963413325155	0.9349430560602794
8	0.17648497560277035	0.7859609245607342
9	0.135892902720237	0.680836003097468
>10	0.5929257870630821	5.975354433832121
>50	0.06821528609433235	2.7015296001462636
>100	0.0764162907908192	9.461720101048044
>500	0.010034276823211325	3.80328896601781
>1k	0.004410671131081901	3.6874592972173645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4381	0.26742684828821794	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2893	0.1765957251992272	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2019	0.12324464886873132	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1995	0.12177963075439276	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1830	0.11170763121831517	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1822	0.11121929184686899	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1770	0.10804508593246877	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1728	0.1054813042323763	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1705	0.10407732853946851	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1658	0.10120833473222215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8312726429231996E-4
2	0.0	0.0	0.0	0.0	1.8312726429231996E-4
3	0.0	0.0	0.0	0.0	1.8312726429231996E-4
4	0.0	0.0	0.0	0.0	1.8312726429231996E-4
5	0.0	0.0	0.0	0.0	1.8312726429231996E-4
6	0.0	0.0	0.0	6.104242143077331E-5	1.8312726429231996E-4
7	0.0	0.0	0.0	6.104242143077331E-5	1.8312726429231996E-4
8	0.0	0.0	0.0	6.104242143077331E-5	1.8312726429231996E-4
9	0.0	0.0	0.0	1.2208484286154662E-4	1.8312726429231996E-4
10	0.0	0.0	0.0	1.2208484286154662E-4	1.8312726429231996E-4
11	0.0	0.0	0.0	1.2208484286154662E-4	1.8312726429231996E-4
12	0.0	0.0	0.0	1.2208484286154662E-4	3.662545285846399E-4
13	0.0	0.0	0.0	1.2208484286154662E-4	4.272969500154132E-4
14	0.0	0.0	0.0	1.2208484286154662E-4	4.272969500154132E-4
15	0.0	0.0	0.0	1.8312726429231996E-4	4.272969500154132E-4
16	0.0	0.0	0.0	1.8312726429231996E-4	4.272969500154132E-4
17	0.0	0.0	0.0	1.8312726429231996E-4	4.272969500154132E-4
18	0.0	0.0	0.0	2.4416968572309324E-4	5.493817928769599E-4
19	0.0	0.0	0.0	2.4416968572309324E-4	5.493817928769599E-4
20	0.0	0.0	0.0	3.052121071538666E-4	5.493817928769599E-4
21	0.0	0.0	0.0	8.545939000308264E-4	6.104242143077332E-4
22	0.0	0.0	0.0	9.76678742892373E-4	6.714666357385064E-4
23	0.0	0.0	0.0	0.001953357485784746	6.714666357385064E-4
24	0.0	0.0	0.0	0.003113163492969439	6.714666357385064E-4
25	0.0	6.104242143077331E-5	0.0	0.0041508846572925855	6.714666357385064E-4
26	0.0	6.104242143077331E-5	0.0	0.005310690664477279	8.545939000308264E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	375	0.0	17.065395	29
CATCGTT	395	0.0	16.201324	28
TCGTTTA	395	0.0	16.20083	30
CGTAGTA	70	3.6816712E-4	16.009071	2
TAGGACG	180	0.0	15.996858	4
TAACGCT	70	3.7031324E-4	15.996857	4
CGTTTAT	405	0.0	15.800811	31
CTATACA	155	1.891749E-10	15.480828	4
TAAACGC	425	0.0	15.434146	28
CGCTTCG	390	0.0	15.177895	32
CCGATAA	180	7.2759576E-12	15.110909	9
TTAGTAC	75	6.246992E-4	14.933134	3
GTAAACG	420	0.0	14.855583	27
GTATAGG	120	5.341135E-7	14.685749	1
AAACGCT	420	0.0	14.475112	29
GGCATCG	470	0.0	14.296808	26
CCTATAC	135	1.3198587E-7	14.222033	3
AACGCTT	420	0.0	14.093759	30
TACCGTC	375	0.0	14.077662	7
CTCGTAC	80	0.0010183728	13.999813	3
>>END_MODULE