FastQCFastQC Report
Thu 2 Feb 2017
SRR4062754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638205
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49850.30429647083240496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35880.21902020809361467No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21270.12983723038325484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20970.12800595774033163No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20330.12409924276876215No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19510.11909376421143875No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18960.1157364310327462No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18630.11372203112553068No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17590.10737361929673025No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17410.10627485571097633No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17200.1049929648609301No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17160.10474879517520701No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16850.10285648011085305No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16760.10230709831797608No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16580.10120833473222215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3600.017.33262328
CATCGTT3650.016.6568528
CTAGCGG3750.016.63931829
ACCGTCG4800.016.0022758
GTATAAT1501.1459633E-1015.9998321
AAACGCT3700.015.99934429
TAGCGGC3950.015.7968230
TAAACGC3750.015.78601928
TACCGTC4800.015.6688957
ATACCGT4750.015.496946
CGTCGTA4800.015.33410810
AACCGCG3050.015.2152787
ATCGTTT4000.015.19937729
CCGTCGT4850.015.1764899
TAACGGC756.2483037E-414.93272128
AACGCTT4100.014.43843230
TATAATG2003.6379788E-1214.4002892
TACACCG901.5329609E-414.2242435
CGCGGTC3150.014.22294110
GTGTAAT2053.6379788E-1214.0486331
Produced by FastQC (version 0.11.5)