##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062754_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1638205
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.154364075314138	32.0	32.0	32.0	32.0	32.0
2	31.249468167903284	32.0	32.0	32.0	32.0	32.0
3	31.342801419846722	32.0	32.0	32.0	32.0	32.0
4	31.448782661510617	32.0	32.0	32.0	32.0	32.0
5	31.352591403395788	32.0	32.0	32.0	32.0	32.0
6	34.87949737670194	36.0	36.0	36.0	36.0	36.0
7	34.90812016811083	36.0	36.0	36.0	36.0	36.0
8	34.8498270973413	36.0	36.0	36.0	32.0	36.0
9	34.965028186338095	36.0	36.0	36.0	36.0	36.0
10	34.79366135495863	36.0	36.0	36.0	32.0	36.0
11	34.9806477211338	36.0	36.0	36.0	36.0	36.0
12	34.876039323527884	36.0	36.0	36.0	32.0	36.0
13	34.933062711931655	36.0	36.0	36.0	36.0	36.0
14	34.877624595212446	36.0	36.0	36.0	32.0	36.0
15	34.843544611327644	36.0	36.0	36.0	32.0	36.0
16	34.85268876605797	36.0	36.0	36.0	32.0	36.0
17	34.821609017186496	36.0	36.0	36.0	32.0	36.0
18	34.82127511514127	36.0	36.0	36.0	32.0	36.0
19	34.8118049938805	36.0	36.0	36.0	32.0	36.0
20	34.79651997155423	36.0	36.0	36.0	32.0	36.0
21	34.7876273116002	36.0	36.0	36.0	32.0	36.0
22	34.76969182733541	36.0	36.0	36.0	32.0	36.0
23	34.711683214249746	36.0	36.0	36.0	32.0	36.0
24	34.68480928821484	36.0	36.0	36.0	32.0	36.0
25	34.66554674170815	36.0	36.0	36.0	32.0	36.0
26	34.59864791036531	36.0	36.0	36.0	32.0	36.0
27	34.58227694336179	36.0	36.0	36.0	32.0	36.0
28	34.53927255746381	36.0	36.0	36.0	32.0	36.0
29	34.50991603614932	36.0	36.0	36.0	32.0	36.0
30	34.48375020220302	36.0	36.0	36.0	32.0	36.0
31	34.47789928610888	36.0	36.0	36.0	32.0	36.0
32	34.44669317942504	36.0	36.0	36.0	32.0	36.0
33	34.40136979193691	36.0	36.0	36.0	32.0	36.0
34	34.39028509862929	36.0	36.0	36.0	32.0	36.0
35	34.35589624009205	36.0	36.0	36.0	32.0	36.0
36	34.327129388568586	36.0	36.0	36.0	32.0	36.0
37	34.31762630440024	36.0	36.0	36.0	32.0	36.0
38	33.876461737084185	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	7.0
21	35.0
22	108.0
23	390.0
24	1121.0
25	2714.0
26	5924.0
27	11540.0
28	20060.0
29	31459.0
30	47962.0
31	69282.0
32	99108.0
33	158478.0
34	379318.0
35	810696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28224466348484	18.751762762399775	12.480174646257083	26.485817927858303
2	15.641265897735632	21.20467218693631	36.7659114701762	26.388150445151858
3	17.66439487121575	25.260696921325476	28.814342527339377	28.2605656801194
4	12.117858481432693	16.823077984277884	36.13840051471064	34.92066301957878
5	14.278999611159302	37.06079161129603	33.01452872446211	15.645680053082556
6	33.624343259046654	36.08575982341627	16.94966985166063	13.34022706587645
7	29.89703937067475	30.707075269854272	21.126310051465055	18.269575308005926
8	28.001428423179526	32.71467552207646	19.57104574006361	19.712850314680406
9	27.798983858079534	14.380672914745046	18.40299800324389	39.41734522393153
10	15.813906925382938	26.717519507798542	31.40744882617473	26.06112474064379
11	37.147503536029035	21.262629438197173	22.38256018879984	19.207306836973952
12	25.16219020876572	23.91911854474423	28.41344158222439	22.50524966426566
13	29.617391739607363	19.553132304116154	25.02894940370638	25.8005265525701
14	23.987413052700973	19.988462982349585	25.08581038392631	30.93831358102313
15	25.36538467407925	26.957920406786695	22.507195375426154	25.1694995437079
16	25.813130835273974	25.716500682149057	23.66895474009663	24.801413742480335
17	24.063777121910874	25.67383202956895	25.249404073360783	25.012986775159398
18	25.08153741442615	24.575373655922185	26.371363779258395	23.971725150393265
19	25.668032999533025	24.973553370915116	25.476115626554673	23.88229800299718
20	25.859339948297066	24.08868242985463	25.167485143800683	24.88449247804762
21	27.176518632329	24.20453900345562	24.335902615124763	24.28303974909062
22	25.736888850906936	24.38424983442243	24.90811589514133	24.9707454195293
23	24.3223825130341	23.975600093517105	25.49702326268478	26.204994130764014
24	24.800925403108888	25.10308538919122	25.195503615237406	24.90048559246248
25	25.00947684749692	24.344296769081733	25.27696506476914	25.36926131865221
26	24.718396049334483	25.153750598978757	25.723032221242153	24.404821130444603
27	25.561529577512935	24.540350286044962	24.840190843142857	25.057929293299246
28	24.42368323866671	24.67548322706865	25.741405990092815	25.15942754417182
29	24.539175500013734	24.719555855341667	25.691595374205306	25.049673270439293
30	24.536916930420798	24.804404821130444	26.00309485076654	24.655583397682218
31	25.22462085025989	24.68079391773313	24.680427663204544	25.414157568802437
32	24.677436584554435	24.969219358993534	24.69117112937636	25.662172927075673
33	24.366181277678923	24.46476478828962	25.46256420899704	25.706489725034416
34	25.081049075054707	24.57213840758635	25.673526817461795	24.673285699897143
35	25.85946203313993	24.48289438745456	25.33626743905677	24.321376140348736
36	24.79237946410858	25.06053882145397	25.0169545325524	25.130127181885054
37	25.633177776896055	24.91922561584173	24.867644769732724	24.57995183752949
38	24.71397012458163	24.788258842158147	25.40558160374508	25.092189429515145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	298.0
1	261.5
2	225.0
3	225.0
4	644.5
5	1064.0
6	1064.0
7	1243.0
8	1422.0
9	1483.0
10	1544.0
11	1544.0
12	1979.0
13	2414.0
14	3014.5
15	3615.0
16	3615.0
17	5587.0
18	7559.0
19	7559.0
20	8805.5
21	10052.0
22	10756.0
23	11460.0
24	11460.0
25	13683.5
26	15907.0
27	15907.0
28	20808.0
29	25709.0
30	32050.0
31	38391.0
32	38391.0
33	48436.5
34	58482.0
35	58482.0
36	64991.5
37	71501.0
38	81276.0
39	91051.0
40	91051.0
41	97133.0
42	103215.0
43	115807.5
44	128400.0
45	128400.0
46	133507.5
47	138615.0
48	138615.0
49	146573.0
50	154531.0
51	153672.0
52	152813.0
53	152813.0
54	145291.5
55	137770.0
56	137770.0
57	133610.0
58	129450.0
59	116092.5
60	102735.0
61	102735.0
62	96877.5
63	91020.0
64	75069.5
65	59119.0
66	59119.0
67	49457.5
68	39796.0
69	39796.0
70	31801.5
71	23807.0
72	18772.0
73	13737.0
74	13737.0
75	10251.5
76	6766.0
77	6766.0
78	6825.5
79	6885.0
80	5271.5
81	3658.0
82	3658.0
83	3529.5
84	3401.0
85	3401.0
86	2181.0
87	961.0
88	799.5
89	638.0
90	638.0
91	382.0
92	126.0
93	77.0
94	28.0
95	28.0
96	24.0
97	20.0
98	20.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009217405636046772
2	0.0
3	0.0
4	1.8312726429231993E-4
5	1.2208484286154665E-4
6	1.2208484286154665E-4
7	3.6625452858463986E-4
8	0.002136484750077066
9	0.002930036228677119
10	8.545939000308265E-4
11	0.0041508846572925855
12	3.052121071538666E-4
13	1.2208484286154665E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.441696857230933E-4
22	0.0
23	1.2208484286154665E-4
24	0.0
25	1.2208484286154665E-4
26	0.0
27	6.104242143077332E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2208484286154665E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1638205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.89375602572082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5104060087863	41.77326910892094
2	14.031037717998379	15.123706471229825
3	4.1764371162671186	6.752516490025953
4	1.6460347946772451	3.548439905367317
5	0.7928209996038869	2.136405076235999
6	0.45095870272050287	1.4582314981256608
7	0.2719604118092004	1.0259877657889727
8	0.18395866918678686	0.7931378908775183
9	0.13106131961394907	0.6357048105314871
>10	0.6381190233799654	6.208559136430333
>50	0.06876481102332822	2.6149865983987928
>100	0.08214786262640525	9.6725201490324
>500	0.011621268218961527	4.302345942289916
>1k	0.004671294088013947	3.954189156744883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4985	0.30429647083240496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3588	0.21902020809361467	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2127	0.12983723038325484	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2097	0.12800595774033163	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2033	0.12409924276876215	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1951	0.11909376421143875	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1896	0.1157364310327462	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1863	0.11372203112553068	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1759	0.10737361929673025	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1741	0.10627485571097633	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1720	0.1049929648609301	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1716	0.10474879517520701	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1685	0.10285648011085305	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1676	0.10230709831797608	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1658	0.10120833473222215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2208484286154662E-4
2	0.0	0.0	0.0	0.0	1.2208484286154662E-4
3	0.0	0.0	0.0	0.0	1.2208484286154662E-4
4	0.0	0.0	0.0	0.0	1.2208484286154662E-4
5	0.0	0.0	0.0	0.0	1.2208484286154662E-4
6	0.0	0.0	0.0	0.0	1.2208484286154662E-4
7	0.0	0.0	0.0	6.104242143077331E-5	1.2208484286154662E-4
8	0.0	0.0	0.0	6.104242143077331E-5	1.2208484286154662E-4
9	0.0	0.0	0.0	1.8312726429231996E-4	1.2208484286154662E-4
10	0.0	0.0	0.0	1.8312726429231996E-4	1.8312726429231996E-4
11	0.0	0.0	0.0	1.8312726429231996E-4	1.8312726429231996E-4
12	0.0	0.0	0.0	1.8312726429231996E-4	3.052121071538666E-4
13	0.0	0.0	0.0	1.8312726429231996E-4	3.052121071538666E-4
14	0.0	0.0	0.0	1.8312726429231996E-4	3.052121071538666E-4
15	0.0	0.0	0.0	1.8312726429231996E-4	5.493817928769599E-4
16	0.0	0.0	0.0	1.8312726429231996E-4	5.493817928769599E-4
17	0.0	0.0	0.0	1.8312726429231996E-4	5.493817928769599E-4
18	0.0	0.0	0.0	2.4416968572309324E-4	6.104242143077332E-4
19	0.0	0.0	0.0	2.4416968572309324E-4	6.104242143077332E-4
20	0.0	0.0	0.0	3.662545285846399E-4	6.104242143077332E-4
21	0.0	0.0	0.0	8.545939000308264E-4	6.104242143077332E-4
22	0.0	0.0	0.0	0.0010987635857539198	6.104242143077332E-4
23	0.0	0.0	0.0	0.0020754423286462927	6.104242143077332E-4
24	0.0	0.0	0.0	0.003113163492969439	6.104242143077332E-4
25	0.0	0.0	0.0	0.0040898422358618125	6.104242143077332E-4
26	0.0	0.0	0.0	0.005249648243046505	6.714666357385064E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	360	0.0	17.332623	28
CATCGTT	365	0.0	16.65685	28
CTAGCGG	375	0.0	16.639318	29
ACCGTCG	480	0.0	16.002275	8
GTATAAT	150	1.1459633E-10	15.999832	1
AAACGCT	370	0.0	15.999344	29
TAGCGGC	395	0.0	15.79682	30
TAAACGC	375	0.0	15.786019	28
TACCGTC	480	0.0	15.668895	7
ATACCGT	475	0.0	15.49694	6
CGTCGTA	480	0.0	15.334108	10
AACCGCG	305	0.0	15.215278	7
ATCGTTT	400	0.0	15.199377	29
CCGTCGT	485	0.0	15.176489	9
TAACGGC	75	6.2483037E-4	14.932721	28
AACGCTT	410	0.0	14.438432	30
TATAATG	200	3.6379788E-12	14.400289	2
TACACCG	90	1.5329609E-4	14.224243	5
CGCGGTC	315	0.0	14.222941	10
GTGTAAT	205	3.6379788E-12	14.048633	1
>>END_MODULE