FastQCFastQC Report
Thu 2 Feb 2017
SRR4062753_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062753_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108703
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA82120.3894336945506313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT71370.3384544907462075No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC65210.30924222140339347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45160.2141600784937471No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA32210.15274792135260395No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30350.14392733353155945No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29800.14131909519737962No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29750.1410819826215451No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28770.1364345761351883No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG27330.12960573395115388No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26780.12699749561697402No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26170.12410472219179278No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26070.12363049704012372No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26010.12334596194912227No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25450.12069030109977555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24470.11604289461341877No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24320.11533155688591519No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22980.1089769398535498No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22670.10750684188337571No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22300.1057522088222002No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT21700.10290685791218583No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21580.10233778773018296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6000.017.06565528
ACGATTA957.700837E-716.84190632
CTAGCGG6100.016.52400229
TCGTAAT600.002437597816.0051252
TAGCGGC6350.015.62186230
GTCCTAT7850.015.5057071
GTTCAAA8250.014.9480461
CCGTTGC756.2491006E-414.93280117
ATCGTTT5600.014.85661429
AGGCCCG7900.014.78463510
TTTTCGG8500.014.68182929
GTTTTCG8600.014.51076728
CCTATTC8100.014.417533
ACCGTCG5900.014.3716888
CGACCGT901.5352987E-414.22205414
CGTTGCT901.5359175E-414.22137818
CTATTCC8100.014.2193554
AGCGGCG7500.014.07983231
TGGATAC8200.014.04894624
CATCGTT5850.013.9478928
Produced by FastQC (version 0.11.5)