##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062753_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108703
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226104861613987	32.0	32.0	32.0	32.0	32.0
2	30.860824402488163	32.0	32.0	32.0	32.0	32.0
3	30.922327136633278	32.0	32.0	32.0	32.0	32.0
4	30.979448504602118	32.0	32.0	32.0	32.0	32.0
5	30.904619569469954	32.0	32.0	32.0	32.0	32.0
6	34.55605649539077	36.0	36.0	36.0	32.0	36.0
7	34.47913717579005	36.0	36.0	36.0	32.0	36.0
8	34.461378392310344	36.0	36.0	36.0	32.0	36.0
9	34.573213487153005	36.0	36.0	36.0	32.0	36.0
10	34.31615832101534	36.0	36.0	36.0	32.0	36.0
11	34.5856709076622	36.0	36.0	36.0	32.0	36.0
12	34.403043956403536	36.0	36.0	36.0	32.0	36.0
13	34.47917558802733	36.0	36.0	36.0	32.0	36.0
14	34.38152124789503	36.0	36.0	36.0	32.0	36.0
15	34.3289192456216	36.0	36.0	36.0	32.0	36.0
16	34.33384644494744	36.0	36.0	36.0	32.0	36.0
17	34.25994699111255	36.0	36.0	36.0	32.0	36.0
18	34.28756491549545	36.0	36.0	36.0	32.0	36.0
19	34.26907013458035	36.0	36.0	36.0	32.0	36.0
20	34.24071336741115	36.0	36.0	36.0	32.0	36.0
21	34.20517019229356	36.0	36.0	36.0	32.0	36.0
22	34.18247377653468	36.0	36.0	36.0	32.0	36.0
23	34.11649103738175	36.0	36.0	36.0	32.0	36.0
24	34.094780535713184	36.0	36.0	36.0	32.0	36.0
25	34.07053292948319	36.0	36.0	36.0	32.0	36.0
26	34.0065907811579	36.0	36.0	36.0	32.0	36.0
27	33.99230759381478	36.0	36.0	36.0	32.0	36.0
28	33.97215255064369	36.0	36.0	36.0	32.0	36.0
29	33.94084468035565	36.0	36.0	36.0	32.0	36.0
30	33.892261262017456	36.0	36.0	36.0	32.0	36.0
31	33.92480496305075	36.0	36.0	36.0	32.0	36.0
32	33.86308123998496	36.0	36.0	36.0	32.0	36.0
33	33.816343980162216	36.0	36.0	36.0	32.0	36.0
34	33.807465062647516	36.0	36.0	36.0	32.0	36.0
35	33.7436182335777	36.0	36.0	36.0	32.0	36.0
36	33.70115089702058	36.0	36.0	36.0	27.0	36.0
37	33.682439395211176	36.0	36.0	36.0	27.0	36.0
38	33.059170494849205	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	5.0
9	9.0
10	20.0
11	28.0
12	12.0
13	11.0
14	217.0
15	521.0
16	742.0
17	985.0
18	1314.0
19	1729.0
20	2394.0
21	3094.0
22	4685.0
23	6589.0
24	9799.0
25	14321.0
26	20564.0
27	29626.0
28	42043.0
29	57302.0
30	77060.0
31	102747.0
32	138815.0
33	205621.0
34	453279.0
35	935167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57693768636165	16.986828860653713	11.831946897612672	25.604286555371964
2	16.63197332590594	19.996792427852164	36.995833477658266	26.375400768583635
3	18.646231833604784	24.902794786252873	27.972003760150937	28.478969619991407
4	12.58147431519842	15.7347389786782	35.53954207451248	36.144244631610896
5	14.83171132139358	36.48915112548122	32.905707692614115	15.773429860511081
6	34.083386130575484	34.96848289179664	17.30481776415377	13.643313213474109
7	30.370753965826385	30.468776304676382	21.076225528203828	18.0842442012934
8	28.951927230960887	31.530246848886446	19.65254405678572	19.86528186336694
9	28.16295734953163	14.456022410026275	18.301189501204295	39.0798307392378
10	16.390199995921645	25.964946059004323	30.6833664265021	26.961487518571932
11	37.46163433011359	21.13981607241769	21.872462838888847	19.526086758579872
12	25.58250705192531	23.498987975210607	28.005059175470116	22.913445797393965
13	29.725921298951008	19.36754206607921	25.057804249554994	25.848732385414785
14	24.78625830743423	19.705829206787946	24.397925921850717	31.109986563927112
15	25.82386281436044	26.264521212080965	22.937241990713446	24.974373982845155
16	26.500700746751782	25.155124699722307	23.588439931419302	24.755734622106605
17	24.43544703430932	25.15461795290052	25.323430068667967	25.086504944122197
18	25.80224758320307	23.666540831347792	26.525484497594586	24.005727087854552
19	25.72517071070097	25.33622823017655	25.175927585843837	23.762673473278642
20	25.984227322329733	23.21943219688293	25.54597091980728	25.25036956098005
21	27.499356199666792	23.937089206776335	23.8033494690485	24.760205124508374
22	26.043943119043284	24.0708754477023	24.477032492563588	25.40814894069083
23	24.175432772112877	23.919563670856057	25.19625326061181	26.708750296419254
24	24.86787342978901	25.256652572217252	24.63153251644023	25.243941481553506
25	25.39194305288231	23.851538317787153	24.71363754077849	26.042881088552043
26	24.56259596042482	24.982509530230885	25.537745975294683	24.917148534049616
27	25.99473659998018	24.607462190357897	24.38659668402953	25.011204525632397
28	24.69825464320301	24.040888452151844	25.514022326073736	25.74683457857141
29	24.37298254567047	24.756150656825742	25.905797273288645	24.965069524215146
30	24.904736589259265	24.443298885957546	26.023426618363516	24.628537906419677
31	25.03164447403778	24.731158318169122	24.338762097579274	25.898435110213825
32	24.569266221906684	24.924912961637673	24.319616343530374	26.186204472925272
33	24.070028731243827	24.393338221470557	25.201351098834913	26.335281948450707
34	25.051435986503805	24.715596225845953	25.57902834599511	24.653939441655133
35	26.256671632595275	24.16974843563763	25.27913783511125	24.294442096655846
36	24.64793842817688	25.29635495148615	24.70594540703777	25.349761213299203
37	25.96603671214582	25.13981423866689	24.634766128909867	24.259382920277428
38	24.653725949568194	25.033363527286006	24.78898221086129	25.52392831228451
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	233.0
1	310.0
2	387.0
3	387.0
4	984.5
5	1582.0
6	1582.0
7	1873.5
8	2165.0
9	2203.0
10	2241.0
11	2241.0
12	2877.0
13	3513.0
14	4296.5
15	5080.0
16	5080.0
17	7383.0
18	9686.0
19	9686.0
20	11332.0
21	12978.0
22	12903.0
23	12828.0
24	12828.0
25	15055.0
26	17282.0
27	17282.0
28	22485.5
29	27689.0
30	35087.5
31	42486.0
32	42486.0
33	55114.0
34	67742.0
35	67742.0
36	75966.5
37	84191.0
38	97759.5
39	111328.0
40	111328.0
41	118844.0
42	126360.0
43	147118.0
44	167876.0
45	167876.0
46	181579.0
47	195282.0
48	195282.0
49	197982.5
50	200683.0
51	195945.5
52	191208.0
53	191208.0
54	182025.5
55	172843.0
56	172843.0
57	170000.0
58	167157.0
59	150104.0
60	133051.0
61	133051.0
62	128349.5
63	123648.0
64	103167.5
65	82687.0
66	82687.0
67	69432.0
68	56177.0
69	56177.0
70	45068.5
71	33960.0
72	26947.0
73	19934.0
74	19934.0
75	14805.5
76	9677.0
77	9677.0
78	9602.5
79	9528.0
80	7404.0
81	5280.0
82	5280.0
83	5601.5
84	5923.0
85	5923.0
86	3930.0
87	1937.0
88	1699.0
89	1461.0
90	1461.0
91	1063.5
92	666.0
93	571.0
94	476.0
95	476.0
96	451.5
97	427.0
98	427.0
99	739.0
100	1051.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12249235667611798
2	0.05643279304861804
3	0.013136036701232937
4	0.0029876184555150728
5	2.3711257583452957E-4
6	3.319576061683414E-4
7	0.0
8	1.8969006066762366E-4
9	1.8969006066762366E-4
10	4.7422515166905914E-4
11	0.002228858212844578
12	0.002797928394847449
13	0.013942219459070338
14	0.009484503033381183
15	0.02342672249245152
16	0.011286558609723607
17	0.02096075170377241
18	0.006022659426197051
19	0.007824715002539477
20	0.00569070182002871
21	0.006164926971697769
22	0.00754017991153804
23	0.0096267705788819
24	0.014606134671407022
25	0.017119527975253034
26	0.019538076248765236
27	0.008536052730043063
28	0.0064968845778661105
29	0.010859755973221455
30	0.0036041111526848497
31	0.00569070182002871
32	0.007113377275035887
33	0.009342235487880464
34	0.01218758639789482
35	0.015933965096080385
36	0.015933965096080385
37	0.00905770039687903
38	0.005358744213860368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2108703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.88645198451411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61457693974748	31.141815673022855
2	14.871397858676483	12.755629804174701
3	5.566416535074602	7.16171566371847
4	2.502850335411776	4.293534829362487
5	1.3066059062511013	2.80178457305602
6	0.7854780308932102	2.0211819514075415
7	0.4880584352417643	1.465176625404311
8	0.3274348617488808	1.1234015581159515
9	0.21851183342391525	0.8434077526964598
>10	1.0745104607234306	8.313188537979038
>50	0.10273865198162242	3.090571175017144
>100	0.11005666868098794	10.324446257209916
>500	0.020059329569608392	5.876000532795701
>1k	0.010971677499399066	7.748687558539319
>5k	3.324750757393656E-4	1.0394575075001375
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8212	0.3894336945506313	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7137	0.3384544907462075	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	6521	0.30924222140339347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4516	0.2141600784937471	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3221	0.15274792135260395	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3035	0.14392733353155945	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2980	0.14131909519737962	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2975	0.1410819826215451	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2877	0.1364345761351883	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2733	0.12960573395115388	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2678	0.12699749561697402	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2617	0.12410472219179278	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2607	0.12363049704012372	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2601	0.12334596194912227	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2545	0.12069030109977555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2447	0.11604289461341877	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2432	0.11533155688591519	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2298	0.1089769398535498	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2267	0.10750684188337571	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2230	0.1057522088222002	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2170	0.10290685791218583	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2158	0.10233778773018296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.484503033381183E-5
2	0.0	0.0	0.0	0.0	9.484503033381183E-5
3	0.0	0.0	0.0	0.0	9.484503033381183E-5
4	0.0	0.0	0.0	0.0	9.484503033381183E-5
5	0.0	0.0	0.0	0.0	1.4226754550071774E-4
6	0.0	0.0	0.0	0.0	1.4226754550071774E-4
7	0.0	0.0	0.0	0.0	1.4226754550071774E-4
8	0.0	0.0	0.0	0.0	1.4226754550071774E-4
9	0.0	0.0	0.0	4.7422515166905914E-5	1.4226754550071774E-4
10	0.0	0.0	0.0	4.7422515166905914E-5	2.3711257583452957E-4
11	0.0	0.0	0.0	4.7422515166905914E-5	2.3711257583452957E-4
12	0.0	0.0	0.0	4.7422515166905914E-5	7.587602426704946E-4
13	0.0	0.0	0.0	4.7422515166905914E-5	9.958728185050243E-4
14	0.0	0.0	0.0	4.7422515166905914E-5	9.958728185050243E-4
15	0.0	0.0	0.0	1.4226754550071774E-4	0.0013278304246733656
16	0.0	0.0	0.0	1.8969006066762366E-4	0.0014226754550071773
17	0.0	0.0	0.0	1.8969006066762366E-4	0.0015175204853409893
18	0.0	0.0	0.0	2.845350910014355E-4	0.0015175204853409893
19	0.0	0.0	0.0	4.2680263650215323E-4	0.0015175204853409893
20	0.0	0.0	0.0	5.21647666835965E-4	0.0017546330611755189
21	0.0	0.0	0.0	7.587602426704946E-4	0.0018969006066762366
22	0.0	0.0	0.0	9.484503033381183E-4	0.0019443231218431425
23	0.0	0.0	0.0	0.0019443231218431425	0.0020391681521769543
24	0.0	0.0	0.0	0.0029401959403481666	0.0020391681521769543
25	0.0	0.0	0.0	0.003983491274020097	0.0020391681521769543
26	0.0	0.0	0.0	0.005216476668359651	0.002276280728011484
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	600	0.0	17.065655	28
ACGATTA	95	7.700837E-7	16.841906	32
CTAGCGG	610	0.0	16.524002	29
TCGTAAT	60	0.0024375978	16.005125	2
TAGCGGC	635	0.0	15.621862	30
GTCCTAT	785	0.0	15.505707	1
GTTCAAA	825	0.0	14.948046	1
CCGTTGC	75	6.2491006E-4	14.932801	17
ATCGTTT	560	0.0	14.856614	29
AGGCCCG	790	0.0	14.784635	10
TTTTCGG	850	0.0	14.681829	29
GTTTTCG	860	0.0	14.510767	28
CCTATTC	810	0.0	14.41753	3
ACCGTCG	590	0.0	14.371688	8
CGACCGT	90	1.5352987E-4	14.222054	14
CGTTGCT	90	1.5359175E-4	14.221378	18
CTATTCC	810	0.0	14.219355	4
AGCGGCG	750	0.0	14.079832	31
TGGATAC	820	0.0	14.048946	24
CATCGTT	585	0.0	13.94789	28
>>END_MODULE