FastQCFastQC Report
Thu 2 Feb 2017
SRR4062753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108703
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT81230.3852130907007767No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA81180.38497597812494216No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC79080.375017249939892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT55890.2650444372678371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT33800.16028810126414197No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33430.15853346820296646No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA31810.1508510207459277No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31280.1483376274420817No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30930.14667783941124No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG29500.13989641974237244No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC29070.1378572515901955No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28540.13534385828634946No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27250.12922635382981862No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26310.12476863740412945No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25960.12310884937328775No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25950.12306142685812084No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25540.1211171037362777No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24770.11746557006842595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.11196455830906485No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23040.10926147494455121No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22940.10878724979288215No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22470.10655839158003758No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21380.10138933742684483No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT21270.10086768976000887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT6700.019.1059076
TACCGTC6900.018.5516727
ACCGTCG7050.017.9299968
TAGGACG4200.017.5247064
TCAGATA7350.016.7619672
ATCGTTT5900.016.54204229
ACCGTTA805.6219687E-516.0008188
GTATTAA2400.016.000061
CGTCGTA7700.016.0000610
CATCGTT6100.015.99967928
CCGTCGT7750.015.8975879
CGTTTAT6150.015.869631
AGATACC7650.015.6870764
GCATCGT6500.015.50738127
CGGCATC6650.015.39818925
GTCGTAG8100.015.20993211
CAGATAC8250.014.9340973
TCTAGCG7150.014.76893428
AGGACGT4350.014.7137465
CTAGCGG7150.014.54516329
Produced by FastQC (version 0.11.5)