##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108703
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14824041128599	32.0	32.0	32.0	32.0	32.0
2	31.279886261839625	32.0	32.0	32.0	32.0	32.0
3	31.362024429234463	32.0	32.0	32.0	32.0	32.0
4	31.469910651239175	32.0	32.0	32.0	32.0	32.0
5	31.380242262660982	32.0	32.0	32.0	32.0	32.0
6	34.90498993931341	36.0	36.0	36.0	36.0	36.0
7	34.93242196743686	36.0	36.0	36.0	36.0	36.0
8	34.88016520107384	36.0	36.0	36.0	32.0	36.0
9	34.989394428708074	36.0	36.0	36.0	36.0	36.0
10	34.82948096531375	36.0	36.0	36.0	32.0	36.0
11	35.014586691440186	36.0	36.0	36.0	36.0	36.0
12	34.90402631380522	36.0	36.0	36.0	32.0	36.0
13	34.958178558099455	36.0	36.0	36.0	36.0	36.0
14	34.89466036705975	36.0	36.0	36.0	32.0	36.0
15	34.86236895380715	36.0	36.0	36.0	32.0	36.0
16	34.860796423204214	36.0	36.0	36.0	32.0	36.0
17	34.82669963479921	36.0	36.0	36.0	32.0	36.0
18	34.82647200672641	36.0	36.0	36.0	32.0	36.0
19	34.815688601002606	36.0	36.0	36.0	32.0	36.0
20	34.80499103003125	36.0	36.0	36.0	32.0	36.0
21	34.78063624891699	36.0	36.0	36.0	32.0	36.0
22	34.75716494926028	36.0	36.0	36.0	32.0	36.0
23	34.683234670790526	36.0	36.0	36.0	32.0	36.0
24	34.65046429013474	36.0	36.0	36.0	32.0	36.0
25	34.62979850647531	36.0	36.0	36.0	32.0	36.0
26	34.55560930107274	36.0	36.0	36.0	32.0	36.0
27	34.53472015736688	36.0	36.0	36.0	32.0	36.0
28	34.4802463884198	36.0	36.0	36.0	32.0	36.0
29	34.43992302377338	36.0	36.0	36.0	32.0	36.0
30	34.41306717920921	36.0	36.0	36.0	32.0	36.0
31	34.40359690293038	36.0	36.0	36.0	32.0	36.0
32	34.36612837369701	36.0	36.0	36.0	32.0	36.0
33	34.323417285411935	36.0	36.0	36.0	32.0	36.0
34	34.297331582494074	36.0	36.0	36.0	32.0	36.0
35	34.24211517695949	36.0	36.0	36.0	32.0	36.0
36	34.214799333998194	36.0	36.0	36.0	32.0	36.0
37	34.19146935343669	36.0	36.0	36.0	32.0	36.0
38	33.733198558545226	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	15.0
21	63.0
22	180.0
23	593.0
24	1531.0
25	3779.0
26	8015.0
27	15642.0
28	27161.0
29	42947.0
30	63799.0
31	90847.0
32	127821.0
33	201737.0
34	481378.0
35	1043193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.877807298192764	18.312163686221915	12.465935854593882	26.344093160991445
2	15.624770297192162	21.077221401022335	36.24991286112838	27.04809544065712
3	17.68475693352739	25.49477095636512	28.40556493730981	28.414907172797687
4	12.083985393844548	16.663394508464933	35.53663394508465	35.71598615260587
5	14.2660406885759	36.97496087636933	32.73604590505999	16.022952529994782
6	34.0709290756666	35.4590368084951	16.91380178669492	13.556232329143386
7	30.04079298296198	30.637512472162893	21.090569717019427	18.231124827855698
8	28.797740739950346	31.763327808484554	19.503901776250736	19.935029675314357
9	28.02142430664796	14.224903053305152	18.18386604022606	39.569806599820836
10	16.172185769648824	26.22835371579133	30.71586151689496	26.883598997664887
11	37.526053930391015	21.045315698942478	21.828202220707794	19.600428149958717
12	25.262081648641292	23.718898996250758	28.012504422168412	23.006514932939535
13	29.789045183331137	19.250077535996148	25.05612467977871	25.90475260089401
14	24.66558827867177	19.642595472193097	24.3932881965834	31.29852805255173
15	25.840718204507702	26.363409166677336	22.73582386898487	25.060048759830096
16	26.41478027715628	25.218167384485813	23.565159989415292	24.80189234894262
17	24.387834607339204	25.268612981534144	25.24831614504271	25.095236266083937
18	25.918585974411762	23.522326283028004	26.51634677809061	24.042740964469626
19	25.79106683112795	25.285116016812232	25.154609255072906	23.769207896986916
20	25.967431165033673	23.155323438151317	25.60237264327883	25.274872753536176
21	27.391908675617195	24.048479088804825	23.852766368711002	24.70684586686698
22	25.91580033594126	24.07746566371161	24.611396015179004	25.395337985168126
23	24.06747092167168	23.654847225851366	25.378183061515124	26.89949879096183
24	24.85323917118722	25.19653075848045	24.700918052471117	25.249312017861214
25	25.3188334250959	23.79400987241921	24.83711551603047	26.050041186454422
26	24.655582127971552	24.732216912481274	25.630920997409305	24.981279962137865
27	26.16457896848393	24.372196735242817	24.477047965999937	24.986176330273317
28	24.75450549460972	23.759723393953536	25.74397627356721	25.741794837869534
29	24.461529195908575	24.41548193368151	26.152378974184604	24.97060989622531
30	24.948131624036197	24.118047918554673	26.244995146305573	24.688825311103553
31	24.997830419931113	24.375409908365476	24.58179269437185	26.044966977331562
32	24.63447910872228	24.616316285413355	24.555378353423883	26.193826252440484
33	24.146216892563817	24.031786363466072	25.454082438351914	26.367914305618196
34	25.08940329671841	24.32352967677288	25.910808681924387	24.676258344584326
35	26.454744930888797	23.87576628856695	25.3582415351996	24.31124724534465
36	24.785187861922708	24.92555850681675	24.941397626882498	25.347856004378045
37	26.103770896138528	24.8826411305907	24.744926146546003	24.26866182672477
38	24.78326704449801	24.536669731744805	25.001363398604166	25.67869982515302
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	162.0
2	199.0
3	199.0
4	790.5
5	1382.0
6	1382.0
7	1766.0
8	2150.0
9	2295.5
10	2441.0
11	2441.0
12	2979.5
13	3518.0
14	4329.0
15	5140.0
16	5140.0
17	7949.0
18	10758.0
19	10758.0
20	12137.0
21	13516.0
22	13303.0
23	13090.0
24	13090.0
25	14750.0
26	16410.0
27	16410.0
28	21918.5
29	27427.0
30	34762.5
31	42098.0
32	42098.0
33	55316.0
34	68534.0
35	68534.0
36	76042.0
37	83550.0
38	96803.5
39	110057.0
40	110057.0
41	117096.0
42	124135.0
43	144575.5
44	165016.0
45	165016.0
46	181664.5
47	198313.0
48	198313.0
49	202376.0
50	206439.0
51	198919.0
52	191399.0
53	191399.0
54	182417.5
55	173436.0
56	173436.0
57	170238.0
58	167040.0
59	150577.5
60	134115.0
61	134115.0
62	128954.0
63	123793.0
64	103310.0
65	82827.0
66	82827.0
67	69751.0
68	56675.0
69	56675.0
70	45575.5
71	34476.0
72	27035.0
73	19594.0
74	19594.0
75	14464.5
76	9335.0
77	9335.0
78	9374.0
79	9413.0
80	7187.0
81	4961.0
82	4961.0
83	4931.5
84	4902.0
85	4902.0
86	3082.5
87	1263.0
88	1077.0
89	891.0
90	891.0
91	526.0
92	161.0
93	111.0
94	61.0
95	61.0
96	43.0
97	25.0
98	25.0
99	31.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00858347524520997
2	0.0
3	0.0
4	1.4226754550071774E-4
5	1.4226754550071774E-4
6	2.3711257583452957E-4
7	3.319576061683414E-4
8	0.002276280728011484
9	0.0033195760616834143
10	0.0012804079095064596
11	0.004552561456022968
12	4.268026365021532E-4
13	2.3711257583452957E-4
14	0.0
15	0.0
16	4.7422515166905914E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	4.7422515166905914E-5
23	9.484503033381183E-5
24	0.0
25	0.0
26	0.0
27	4.7422515166905914E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.484503033381183E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2108703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.500993098559654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33319674158865	28.4856431583072
2	16.104347178353322	13.044841078545932
3	5.958057056374158	7.239216831631033
4	2.734427675479129	4.429881456523628
5	1.4532975341128982	2.9429996699630125
6	0.8450549995443599	2.053534002266967
7	0.5211445459225262	1.4774810160432166
8	0.3607338081440944	1.1688061979248885
9	0.24949205838377858	0.9094208521272171
>10	1.1777808314838287	8.570843461369877
>50	0.10672497250029758	3.029310674379424
>100	0.11983964507718366	10.522328072880956
>500	0.02307206162300731	6.37513115875667
>1k	0.012360033012325343	8.332785909696074
>5k	4.708584004695369E-4	1.4177764595839473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8123	0.3852130907007767	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8118	0.38497597812494216	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	7908	0.375017249939892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5589	0.2650444372678371	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3380	0.16028810126414197	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3343	0.15853346820296646	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3181	0.1508510207459277	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3128	0.1483376274420817	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3093	0.14667783941124	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2950	0.13989641974237244	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2907	0.1378572515901955	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2854	0.13534385828634946	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2725	0.12922635382981862	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2631	0.12476863740412945	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2596	0.12310884937328775	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2595	0.12306142685812084	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2554	0.1211171037362777	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2477	0.11746557006842595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2361	0.11196455830906485	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2304	0.10926147494455121	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2294	0.10878724979288215	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2247	0.10655839158003758	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2138	0.10138933742684483	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	2127	0.10086768976000887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4226754550071774E-4
2	0.0	0.0	0.0	0.0	1.4226754550071774E-4
3	0.0	0.0	0.0	0.0	1.4226754550071774E-4
4	0.0	0.0	0.0	0.0	1.4226754550071774E-4
5	0.0	0.0	0.0	0.0	1.4226754550071774E-4
6	0.0	0.0	0.0	0.0	3.319576061683414E-4
7	0.0	0.0	0.0	0.0	3.319576061683414E-4
8	0.0	0.0	0.0	0.0	3.793801213352473E-4
9	0.0	0.0	0.0	0.0	3.793801213352473E-4
10	0.0	0.0	0.0	0.0	4.2680263650215323E-4
11	0.0	0.0	0.0	0.0	4.2680263650215323E-4
12	0.0	0.0	0.0	0.0	0.00104329533367193
13	0.0	0.0	0.0	0.0	0.001138140364005742
14	0.0	0.0	0.0	0.0	0.001138140364005742
15	0.0	0.0	0.0	9.484503033381183E-5	0.0013278304246733656
16	0.0	0.0	0.0	1.4226754550071774E-4	0.0014700979701740833
17	0.0	0.0	0.0	1.8969006066762366E-4	0.0014700979701740833
18	0.0	0.0	0.0	3.319576061683414E-4	0.0015649430005078952
19	0.0	0.0	0.0	4.2680263650215323E-4	0.0015649430005078952
20	0.0	0.0	0.0	5.69070182002871E-4	0.001612365515674801
21	0.0	0.0	0.0	8.536052730043065E-4	0.0018494780915093306
22	0.0	0.0	0.0	0.001138140364005742	0.0018969006066762366
23	0.0	0.0	0.0	0.0019917456370100485	0.0020391681521769543
24	0.0	0.0	0.0	0.0031298860010157904	0.0020391681521769543
25	0.0	0.0	0.0	0.004220603849854627	0.0020391681521769543
26	0.0	0.0	0.0	0.00545358924419418	0.0020391681521769543
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	670	0.0	19.105907	6
TACCGTC	690	0.0	18.551672	7
ACCGTCG	705	0.0	17.929996	8
TAGGACG	420	0.0	17.524706	4
TCAGATA	735	0.0	16.761967	2
ATCGTTT	590	0.0	16.542042	29
ACCGTTA	80	5.6219687E-5	16.000818	8
GTATTAA	240	0.0	16.00006	1
CGTCGTA	770	0.0	16.00006	10
CATCGTT	610	0.0	15.999679	28
CCGTCGT	775	0.0	15.897587	9
CGTTTAT	615	0.0	15.8696	31
AGATACC	765	0.0	15.687076	4
GCATCGT	650	0.0	15.507381	27
CGGCATC	665	0.0	15.398189	25
GTCGTAG	810	0.0	15.209932	11
CAGATAC	825	0.0	14.934097	3
TCTAGCG	715	0.0	14.768934	28
AGGACGT	435	0.0	14.713746	5
CTAGCGG	715	0.0	14.545163	29
>>END_MODULE