FastQCFastQC Report
Thu 2 Feb 2017
SRR4062752_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062752_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912544
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT92820.18894487255483106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54040.11000410378003739No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT50340.10247236462411329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73800.019.6831361
GGTATCA29250.015.1669171
ATACCGT9850.015.1040966
TCTAGCG9400.014.97761328
ACCGTCG9500.014.65094958
CTAGCGG9700.014.51453529
CAAGACG14500.014.4523364
TACCGTC10000.014.3983477
TAGGACG7350.014.3645284
GTCCTAT14750.014.1154491
AAGACGG15150.013.9382835
AACGCAG101800.013.9230366
CGTCGTA10250.013.89306710
GTCCTAC8350.013.8098641
TATCAAC104650.013.7648782
ATCGTTT8900.013.66204729
TCAACGC105500.013.4798194
CCGTCGT10650.013.3711269
CAACGCA107350.013.2628245
ATCAACG108150.013.1955083
Produced by FastQC (version 0.11.5)