##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062752_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4912544
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26445422168229	32.0	32.0	32.0	32.0	32.0
2	30.89829668701186	32.0	32.0	32.0	32.0	32.0
3	30.94410920288958	32.0	32.0	32.0	32.0	32.0
4	31.005993432323457	32.0	32.0	32.0	32.0	32.0
5	30.92788420826358	32.0	32.0	32.0	32.0	32.0
6	34.57532248057218	36.0	36.0	36.0	32.0	36.0
7	34.51848451637278	36.0	36.0	36.0	32.0	36.0
8	34.50300923513357	36.0	36.0	36.0	32.0	36.0
9	34.63143332660226	36.0	36.0	36.0	32.0	36.0
10	34.37401273148902	36.0	36.0	36.0	32.0	36.0
11	34.6116559973814	36.0	36.0	36.0	32.0	36.0
12	34.44799720877818	36.0	36.0	36.0	32.0	36.0
13	34.52032979246598	36.0	36.0	36.0	32.0	36.0
14	34.431143619273435	36.0	36.0	36.0	32.0	36.0
15	34.38099709641278	36.0	36.0	36.0	32.0	36.0
16	34.39562821218497	36.0	36.0	36.0	32.0	36.0
17	34.332923430304135	36.0	36.0	36.0	32.0	36.0
18	34.35487621077796	36.0	36.0	36.0	32.0	36.0
19	34.34145261599693	36.0	36.0	36.0	32.0	36.0
20	34.32229675703668	36.0	36.0	36.0	32.0	36.0
21	34.31033004488102	36.0	36.0	36.0	32.0	36.0
22	34.28773523453429	36.0	36.0	36.0	32.0	36.0
23	34.237376398053634	36.0	36.0	36.0	32.0	36.0
24	34.22982348860387	36.0	36.0	36.0	32.0	36.0
25	34.20314159018219	36.0	36.0	36.0	32.0	36.0
26	34.157706068383305	36.0	36.0	36.0	32.0	36.0
27	34.16708532279813	36.0	36.0	36.0	32.0	36.0
28	34.15321572692275	36.0	36.0	36.0	32.0	36.0
29	34.12371146192278	36.0	36.0	36.0	32.0	36.0
30	34.09819250473889	36.0	36.0	36.0	32.0	36.0
31	34.1161331074083	36.0	36.0	36.0	32.0	36.0
32	34.07490742067654	36.0	36.0	36.0	32.0	36.0
33	34.045457506334806	36.0	36.0	36.0	32.0	36.0
34	34.05043333963014	36.0	36.0	36.0	32.0	36.0
35	34.022143720239455	36.0	36.0	36.0	32.0	36.0
36	33.98066989323658	36.0	36.0	36.0	32.0	36.0
37	33.9828058537491	36.0	36.0	36.0	32.0	36.0
38	33.42038788863774	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	12.0
8	12.0
9	17.0
10	42.0
11	53.0
12	31.0
13	29.0
14	370.0
15	885.0
16	1306.0
17	1575.0
18	2116.0
19	2845.0
20	3810.0
21	5705.0
22	8575.0
23	13356.0
24	20643.0
25	31080.0
26	45827.0
27	65703.0
28	92625.0
29	123727.0
30	166689.0
31	224372.0
32	305319.0
33	459537.0
34	1037507.0
35	2298774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73288668897782	17.59766260694896	11.845178688652553	25.824272015420664
2	16.78318331284745	19.536506455853857	37.55865972420943	26.12165050708927
3	18.7578202203363	23.62715306723649	28.519165316556823	29.095861395870394
4	12.697876318191554	15.73896896270101	35.94946840778013	35.61368631132731
5	14.937449771319555	36.134136123693445	33.504555288393036	15.423858816593969
6	34.07871300531498	35.58160459070988	17.001992863147912	13.337689540827233
7	30.48585417250207	30.449783248760724	20.795111453454666	18.269251125282544
8	28.120022285909823	32.73791603130042	19.372151284442346	19.769910398347406
9	27.336264204230282	14.632634528867285	18.750774803955515	39.28032646294691
10	15.960614136439844	26.608684252738513	31.395164685200523	26.035536925621127
11	36.95933536708746	21.414856936267334	22.626132179904594	18.99967551674061
12	25.15826794943487	23.59261680194007	28.50453658366781	22.74457866495725
13	29.11829741594749	19.80426244574015	25.299031043932946	25.778409094379413
14	23.80694123294649	19.939686948675885	25.106971892318423	31.146399926059203
15	25.174667696666848	27.346260460006118	22.288094905530603	25.19097693779643
16	25.872232100649477	25.992306906153946	23.60671083389082	24.52875015930576
17	24.137759725736384	25.955867876051187	25.149023338998294	24.75734905921414
18	24.812901153302068	25.144806557818907	26.065445052290777	23.976847236588256
19	25.50662319335067	25.028073349820403	25.523601564453074	23.941701892375853
20	25.910035120519648	24.456315952761152	24.893856718377076	24.73979220834212
21	26.91596513260526	24.313532134511323	24.580764140628325	24.189738592255097
22	26.05707692188679	24.28499130121099	24.994757926117497	24.663173850784723
23	24.532209126014042	24.363602457968447	25.551748118494533	25.552440297522978
24	24.858574634277037	25.077639909621325	25.2388442637269	24.824941192374737
25	24.936956064087575	24.659473163215488	25.272179188517757	25.13139158417918
26	24.6583980174136	25.48662707031813	25.589608523883633	24.26536638838464
27	25.23494567387059	24.985927587283058	24.876361257818207	24.90276548102815
28	24.542433332281536	24.920204346901155	25.620211573754105	24.9171507470632
29	24.536245887094772	25.05632150632596	25.555427976265367	24.8520046303139
30	24.527382760979965	25.11846093308354	25.739280295758938	24.614876010177554
31	25.212174511372638	24.892950859385657	24.666739953137373	25.228134676104336
32	24.759562288650656	25.02176729021106	24.68631589147563	25.532354529662655
33	24.5137721137599	24.76447955049775	25.217116915372245	25.504631420370107
34	25.187737101688505	24.803997294745315	25.375708327615953	24.63255727595023
35	25.522031040393312	24.665937391904155	25.39289117946043	24.419140388242102
36	24.530890976306242	25.175649940408302	25.105532479409614	25.187926603875844
37	25.477158504387276	24.981540282493224	24.83416791410994	24.707133299009556
38	24.58231198568305	24.979464474201983	25.31141221513129	25.126811324983677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	193.5
2	322.0
3	322.0
4	720.5
5	1119.0
6	1119.0
7	1443.0
8	1767.0
9	1892.0
10	2017.0
11	2017.0
12	3026.5
13	4036.0
14	5786.0
15	7536.0
16	7536.0
17	11647.0
18	15758.0
19	15758.0
20	20853.0
21	25948.0
22	30232.0
23	34516.0
24	34516.0
25	42708.5
26	50901.0
27	50901.0
28	65956.0
29	81011.0
30	99113.0
31	117215.0
32	117215.0
33	146874.0
34	176533.0
35	176533.0
36	199306.5
37	222080.0
38	251769.5
39	281459.0
40	281459.0
41	300530.5
42	319602.0
43	357284.5
44	394967.0
45	394967.0
46	401545.5
47	408124.0
48	408124.0
49	431597.0
50	455070.0
51	458922.5
52	462775.0
53	462775.0
54	444658.0
55	426541.0
56	426541.0
57	405362.0
58	384183.0
59	344567.5
60	304952.0
61	304952.0
62	283748.5
63	262545.0
64	218525.0
65	174505.0
66	174505.0
67	145871.5
68	117238.0
69	117238.0
70	94650.5
71	72063.0
72	55615.5
73	39168.0
74	39168.0
75	30055.0
76	20942.0
77	20942.0
78	19335.0
79	17728.0
80	14026.0
81	10324.0
82	10324.0
83	9863.0
84	9402.0
85	9402.0
86	6356.0
87	3310.0
88	2825.0
89	2340.0
90	2340.0
91	1698.0
92	1056.0
93	937.5
94	819.0
95	819.0
96	713.5
97	608.0
98	608.0
99	1303.5
100	1999.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11971394047564765
2	0.05412674166378968
3	0.012172918960115166
4	0.0026869988340053547
5	2.4427262127321405E-4
6	2.8498472481874973E-4
7	0.0
8	4.6818919077366026E-4
9	2.239165695004462E-4
10	4.0712103545535673E-4
11	0.0024020141091866047
12	0.002992339610596872
13	0.015531667502621858
14	0.010300162197020525
15	0.024793671059231224
16	0.012335767374297308
17	0.021679195137997747
18	0.006127171583603119
19	0.008386693330380349
20	0.006432512360194637
21	0.006778565240331689
22	0.007450314948833028
23	0.010585146921839275
24	0.015592735657940162
25	0.018116886077763374
26	0.01994893073731248
27	0.009180579349518294
28	0.006290019997785262
29	0.011521525303386595
30	0.003358748542506693
31	0.006391800256649101
32	0.007267110482878118
33	0.00903808698710892
34	0.012661464202661595
35	0.016590182194805785
36	0.015999856693395518
37	0.010116957731065615
38	0.006615716826149547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4912544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.95202650826081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2594752151501	31.583057923279206
2	15.21290472696778	13.677017931086036
3	6.09025549267889	8.213079790469473
4	2.890634169517645	5.197594552553666
5	1.614653467784756	3.6290972742757797
6	0.9867694584865255	2.661437211325708
7	0.6018229182848879	1.8937211843214814
8	0.4405034106461476	1.5841216793875943
9	0.31235106962470494	1.263673220548802
>10	1.4303970443420828	11.146671464131385
>50	0.0792287845223509	2.4691422218671435
>100	0.06092136745333546	5.775974436112429
>500	0.011586275260800609	3.6228283941963966
>1k	0.008360290200589003	6.8798103045976955
>5k	1.3630907935737645E-4	0.4027724118472774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9282	0.18894487255483106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5404	0.11000410378003739	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5034	0.10247236462411329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0178025886383918E-4
2	0.0	0.0	0.0	0.0	1.0178025886383918E-4
3	0.0	0.0	0.0	0.0	1.0178025886383918E-4
4	0.0	0.0	0.0	2.0356051772767835E-5	1.0178025886383918E-4
5	0.0	0.0	0.0	2.0356051772767835E-5	1.2213631063660702E-4
6	0.0	0.0	0.0	2.0356051772767835E-5	1.8320446595491052E-4
7	0.0	0.0	0.0	2.0356051772767835E-5	1.8320446595491052E-4
8	0.0	0.0	0.0	2.0356051772767835E-5	1.8320446595491052E-4
9	0.0	0.0	0.0	2.0356051772767835E-5	1.8320446595491052E-4
10	0.0	0.0	0.0	2.0356051772767835E-5	2.0356051772767836E-4
11	0.0	0.0	0.0	2.0356051772767835E-5	2.239165695004462E-4
12	0.0	0.0	0.0	2.0356051772767835E-5	5.292573460919638E-4
13	0.0	0.0	0.0	4.071210354553567E-5	6.106815531830351E-4
14	0.0	0.0	0.0	4.071210354553567E-5	6.106815531830351E-4
15	0.0	0.0	0.0	4.071210354553567E-5	6.513936567285707E-4
16	0.0	0.0	0.0	6.106815531830351E-5	6.717497085013386E-4
17	0.0	0.0	0.0	6.106815531830351E-5	7.124618120468743E-4
18	0.0	0.0	0.0	8.142420709107134E-5	7.328178638196421E-4
19	0.0	0.0	0.0	1.0178025886383918E-4	7.328178638196421E-4
20	0.0	0.0	0.0	1.2213631063660702E-4	8.345981226834813E-4
21	0.0	0.0	0.0	1.2213631063660702E-4	8.75310226229017E-4
22	0.0	0.0	0.0	1.4249236240937487E-4	0.0010178025886383919
23	0.0	0.0	0.0	3.6640893190982105E-4	0.0010178025886383919
24	0.0	0.0	0.0	5.292573460919638E-4	0.0010178025886383919
25	0.0	0.0	0.0	6.513936567285707E-4	0.0010178025886383919
26	0.0	0.0	0.0	8.549541744562491E-4	0.001139938899274999
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7380	0.0	19.683136	1
GGTATCA	2925	0.0	15.166917	1
ATACCGT	985	0.0	15.104096	6
TCTAGCG	940	0.0	14.977613	28
ACCGTCG	950	0.0	14.6509495	8
CTAGCGG	970	0.0	14.514535	29
CAAGACG	1450	0.0	14.452336	4
TACCGTC	1000	0.0	14.398347	7
TAGGACG	735	0.0	14.364528	4
GTCCTAT	1475	0.0	14.115449	1
AAGACGG	1515	0.0	13.938283	5
AACGCAG	10180	0.0	13.923036	6
CGTCGTA	1025	0.0	13.893067	10
GTCCTAC	835	0.0	13.809864	1
TATCAAC	10465	0.0	13.764878	2
ATCGTTT	890	0.0	13.662047	29
TCAACGC	10550	0.0	13.479819	4
CCGTCGT	1065	0.0	13.371126	9
CAACGCA	10735	0.0	13.262824	5
ATCAACG	10815	0.0	13.195508	3
>>END_MODULE