FastQCFastQC Report
Thu 2 Feb 2017
SRR4062752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4912544
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT91820.18690926737755426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT60910.1239887113479289No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG49570.10090494863761017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70650.018.9108351
GGTATCA29150.015.6438161
CGTCTTA5350.015.55088115
ACCGTCG10800.015.4087758
TACCGTC10800.015.2606147
ATACCGT11550.014.68528656
CGTCGTA11500.014.47011410
CGCGTAA6200.013.93601110
TCGCGTA6300.013.7150859
CCGTCGT12200.013.6401399
CTAGCGG10300.013.514111529
TCTAGCG10250.013.42394228
GCGTAAC6750.013.27457711
AACGCAG101350.013.1990016
AATAGCG9950.013.1870995
AACCGCG7700.013.0920727
GTCGTAG12750.013.05147411
TAGCGTA9800.012.8991057
GTATTAG10450.012.8617321
TATCAAC106250.012.6945512
Produced by FastQC (version 0.11.5)