##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4912544
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22900700736726	32.0	32.0	32.0	32.0	32.0
2	31.27735059472241	32.0	32.0	32.0	32.0	32.0
3	31.360746285427673	32.0	32.0	32.0	32.0	32.0
4	31.45580782584339	32.0	32.0	32.0	32.0	32.0
5	31.381955255769718	32.0	32.0	32.0	32.0	32.0
6	34.89194417393514	36.0	36.0	36.0	36.0	36.0
7	34.92262990417999	36.0	36.0	36.0	36.0	36.0
8	34.86210098067315	36.0	36.0	36.0	32.0	36.0
9	34.975303223747204	36.0	36.0	36.0	36.0	36.0
10	34.81515056150133	36.0	36.0	36.0	32.0	36.0
11	34.99676603405486	36.0	36.0	36.0	36.0	36.0
12	34.89999926718214	36.0	36.0	36.0	32.0	36.0
13	34.95410137802328	36.0	36.0	36.0	36.0	36.0
14	34.90066816704339	36.0	36.0	36.0	32.0	36.0
15	34.867068875108295	36.0	36.0	36.0	32.0	36.0
16	34.88668864848844	36.0	36.0	36.0	32.0	36.0
17	34.851492831412806	36.0	36.0	36.0	32.0	36.0
18	34.85303541301615	36.0	36.0	36.0	32.0	36.0
19	34.84589715634099	36.0	36.0	36.0	32.0	36.0
20	34.833033149423194	36.0	36.0	36.0	32.0	36.0
21	34.82915572868151	36.0	36.0	36.0	32.0	36.0
22	34.81225755942339	36.0	36.0	36.0	32.0	36.0
23	34.75753540324524	36.0	36.0	36.0	32.0	36.0
24	34.7369430177114	36.0	36.0	36.0	32.0	36.0
25	34.7233936225304	36.0	36.0	36.0	32.0	36.0
26	34.65852193893836	36.0	36.0	36.0	32.0	36.0
27	34.64202356253705	36.0	36.0	36.0	32.0	36.0
28	34.61953501078057	36.0	36.0	36.0	32.0	36.0
29	34.58882139274478	36.0	36.0	36.0	32.0	36.0
30	34.5669644892748	36.0	36.0	36.0	32.0	36.0
31	34.56292096315066	36.0	36.0	36.0	32.0	36.0
32	34.53852525290359	36.0	36.0	36.0	32.0	36.0
33	34.51083247295088	36.0	36.0	36.0	32.0	36.0
34	34.50890719757421	36.0	36.0	36.0	32.0	36.0
35	34.48117044854967	36.0	36.0	36.0	32.0	36.0
36	34.455201215500566	36.0	36.0	36.0	32.0	36.0
37	34.455761821166384	36.0	36.0	36.0	32.0	36.0
38	34.03478625331397	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	9.0
20	21.0
21	92.0
22	374.0
23	1148.0
24	3191.0
25	7901.0
26	16952.0
27	33040.0
28	56702.0
29	90256.0
30	135947.0
31	196996.0
32	282925.0
33	452888.0
34	1108014.0
35	2526088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19108235130297	18.807793486373043	12.499150058366052	26.501974103957938
2	15.815593712748424	20.611601646723166	36.797471941218234	26.775332699310173
3	17.681653334809823	24.437195880586515	28.877461453780363	29.003689330823295
4	12.216548977889818	16.71365871740709	35.850161210112994	35.219631094590106
5	14.449810301876301	36.67246000135979	33.17608276936868	15.701646927395227
6	34.10257636841264	35.988344103400784	16.646517531172805	13.26256199701376
7	30.314701770413745	30.657865488290742	20.753547463584912	18.2738852777106
8	28.11053922825943	32.95806065758114	19.123150507244073	19.80824960691536
9	27.314168295894348	14.465852174968061	18.49828228781837	39.72169724131922
10	15.756546966679313	26.859967951171132	31.366345961245017	26.01713912090453
11	37.16246036489539	21.370120627517146	22.437357399122856	19.03006160846461
12	25.05130247524138	23.70753487357128	28.451769566441413	22.789393084745928
13	29.15389236220596	19.826358347662335	25.25331646762127	25.76643282251043
14	23.753741442315835	19.88607124943817	25.04712425985396	31.31306304839204
15	25.128243126168435	27.50086309659516	22.10946100431874	25.261432772917658
16	25.87352416050099	26.09837715415417	23.404599206561652	24.623499478783188
17	24.201920634196863	26.047990613417404	24.958473654383553	24.791615098002175
18	24.826464658637153	25.215346671704108	25.931635421484266	24.02655324817447
19	25.54248877974426	25.087653158933538	25.402724128272443	23.967133933049762
20	25.934648117146637	24.496411635193496	24.733579994398013	24.835360253261854
21	26.95643111041086	24.384809330892235	24.458111503168826	24.200648055528077
22	26.00268415171869	24.445749947226883	24.895354520340703	24.65621138071372
23	24.58456801444956	24.378991954666194	25.420510827704707	25.615929203179537
24	24.862515226326725	25.15989678667509	25.10839597568999	24.869192011308193
25	24.925125306842222	24.718714815815275	25.258537282436933	25.097622594905566
26	24.81177769065384	25.45315643102899	25.46907486999602	24.26599100832115
27	25.293493793434763	24.966249638679784	24.803889637539847	24.936366930345606
28	24.558477237048663	24.944265130246162	25.563170528345395	24.93408710435978
29	24.659687526462868	24.9819848941811	25.477573330640908	24.880754248715125
30	24.575250623709426	25.068966303406135	25.677225486428213	24.678557586456222
31	25.26238950735098	24.839390751512862	24.670109824970524	25.228109916165636
32	24.917395141906105	24.96368480363738	24.626303601555527	25.49261645290098
33	24.578629728303707	24.738363666564613	25.1992857468554	25.483720858276282
34	25.182939837281864	24.721305295179036	25.434479569037954	24.661275298501142
35	25.59924145208674	24.67442530794635	25.301310278340512	24.4250229616264
36	24.638619012878053	25.14133206745833	25.06552613065654	25.154522789007082
37	25.52746601353596	24.954402444029	24.7496612752985	24.76847026713654
38	24.702083911360738	24.85750246155706	25.25741769890572	25.182995928176478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	63.0
2	108.0
3	108.0
4	422.0
5	736.0
6	736.0
7	1016.5
8	1297.0
9	1523.0
10	1749.0
11	1749.0
12	2633.5
13	3518.0
14	5409.5
15	7301.0
16	7301.0
17	11820.0
18	16339.0
19	16339.0
20	20850.0
21	25361.0
22	29802.0
23	34243.0
24	34243.0
25	42598.0
26	50953.0
27	50953.0
28	65961.5
29	80970.0
30	99201.0
31	117432.0
32	117432.0
33	148215.0
34	178998.0
35	178998.0
36	200902.5
37	222807.0
38	252253.5
39	281700.0
40	281700.0
41	301073.0
42	320446.0
43	358306.0
44	396166.0
45	396166.0
46	402030.0
47	407894.0
48	407894.0
49	431736.5
50	455579.0
51	458301.5
52	461024.0
53	461024.0
54	444971.0
55	428918.0
56	428918.0
57	407765.0
58	386612.0
59	345869.5
60	305127.0
61	305127.0
62	284091.5
63	263056.0
64	218896.0
65	174736.0
66	174736.0
67	146628.0
68	118520.0
69	118520.0
70	95402.0
71	72284.0
72	55756.5
73	39229.0
74	39229.0
75	29699.0
76	20169.0
77	20169.0
78	18800.0
79	17431.0
80	13474.5
81	9518.0
82	9518.0
83	8804.0
84	8090.0
85	8090.0
86	5204.0
87	2318.0
88	1876.0
89	1434.0
90	1434.0
91	855.0
92	276.0
93	186.0
94	96.0
95	96.0
96	74.0
97	52.0
98	52.0
99	45.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008997374883563385
2	0.0
3	0.0
4	2.239165695004462E-4
5	4.071210354553567E-5
6	1.8320446595491052E-4
7	4.0712103545535673E-4
8	0.0023613020056410692
9	0.003989786147462496
10	8.142420709107135E-4
11	0.0047226040112821385
12	2.646286730459819E-4
13	2.646286730459819E-4
14	0.0
15	0.0
16	2.0356051772767835E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	4.071210354553567E-5
22	1.0178025886383918E-4
23	1.4249236240937487E-4
24	0.0
25	6.106815531830351E-5
26	4.071210354553567E-5
27	8.142420709107134E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.106815531830351E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4912544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.640675218603704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.44285342718469	28.758088088044765
2	16.622395089187737	14.175803007067348
3	6.611637328266472	8.45774039933422
4	3.238270080912585	5.523280911612603
5	1.8075213099295453	3.853696456370543
6	1.0943215395475479	2.799756561154163
7	0.6606093128926258	1.9718179010207586
8	0.47168477660777713	1.6090365891913494
9	0.33311595149066575	1.2783860187884661
>10	1.5379608098993485	11.433907891190694
>50	0.0907243532156037	2.641128870922232
>100	0.06709564967789242	6.002590503230397
>500	0.012606875018580108	3.8052288699411387
>1k	0.009107626417208846	7.377361557980258
>5k	9.586975176008234E-5	0.31217637415088
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9182	0.18690926737755426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6091	0.1239887113479289	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4957	0.10090494863761017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6284841418214268E-4
2	0.0	0.0	0.0	0.0	1.6284841418214268E-4
3	0.0	0.0	0.0	0.0	1.6284841418214268E-4
4	0.0	0.0	0.0	0.0	1.6284841418214268E-4
5	0.0	0.0	0.0	0.0	2.239165695004462E-4
6	0.0	0.0	0.0	0.0	2.8498472481874973E-4
7	0.0	0.0	0.0	0.0	3.2569682836428536E-4
8	0.0	0.0	0.0	0.0	3.2569682836428536E-4
9	0.0	0.0	0.0	0.0	3.2569682836428536E-4
10	0.0	0.0	0.0	0.0	3.2569682836428536E-4
11	2.0356051772767835E-5	0.0	0.0	0.0	3.2569682836428536E-4
12	2.0356051772767835E-5	0.0	0.0	0.0	7.328178638196421E-4
13	2.0356051772767835E-5	0.0	0.0	0.0	8.142420709107135E-4
14	2.0356051772767835E-5	0.0	0.0	0.0	8.142420709107135E-4
15	2.0356051772767835E-5	0.0	0.0	0.0	9.363783815473205E-4
16	2.0356051772767835E-5	0.0	0.0	2.0356051772767835E-5	9.567344333200884E-4
17	2.0356051772767835E-5	0.0	0.0	2.0356051772767835E-5	9.770904850928562E-4
18	2.0356051772767835E-5	0.0	0.0	4.071210354553567E-5	0.0010585146921839276
19	2.0356051772767835E-5	0.0	0.0	8.142420709107134E-5	0.0010585146921839276
20	2.0356051772767835E-5	0.0	0.0	1.0178025886383918E-4	0.001139938899274999
21	2.0356051772767835E-5	0.0	0.0	1.0178025886383918E-4	0.0012010070545933023
22	2.0356051772767835E-5	0.0	0.0	1.4249236240937487E-4	0.001241719158138838
23	2.0356051772767835E-5	0.0	0.0	3.2569682836428536E-4	0.001241719158138838
24	2.0356051772767835E-5	0.0	0.0	4.6818919077366026E-4	0.001262075209911606
25	2.0356051772767835E-5	0.0	0.0	6.106815531830351E-4	0.0013027873134571414
26	2.0356051772767835E-5	0.0	0.0	7.735299673651778E-4	0.0014045675723209808
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7065	0.0	18.910835	1
GGTATCA	2915	0.0	15.643816	1
CGTCTTA	535	0.0	15.550881	15
ACCGTCG	1080	0.0	15.408775	8
TACCGTC	1080	0.0	15.260614	7
ATACCGT	1155	0.0	14.6852865	6
CGTCGTA	1150	0.0	14.470114	10
CGCGTAA	620	0.0	13.936011	10
TCGCGTA	630	0.0	13.715085	9
CCGTCGT	1220	0.0	13.640139	9
CTAGCGG	1030	0.0	13.5141115	29
TCTAGCG	1025	0.0	13.423942	28
GCGTAAC	675	0.0	13.274577	11
AACGCAG	10135	0.0	13.199001	6
AATAGCG	995	0.0	13.187099	5
AACCGCG	770	0.0	13.092072	7
GTCGTAG	1275	0.0	13.051474	11
TAGCGTA	980	0.0	12.899105	7
GTATTAG	1045	0.0	12.861732	1
TATCAAC	10625	0.0	12.694551	2
>>END_MODULE