FastQCFastQC Report
Thu 2 Feb 2017
SRR4062751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091401
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34010.31161781966481616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21600.19791075874037134No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15470.14174441841266408No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13400.12277797070004517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12250.11224105530414578No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11690.10711003563309912No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11630.10656028352548698No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11380.1042696497437697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT1650.018.4212364
TAACGCA450.00885831917.7748784
CGCGTAA1453.6379788E-1217.65471610
TCGCGTA1453.6379788E-1217.6547169
GCGTAAC1505.456968E-1217.06622711
CTATATA957.620165E-716.8571191
GTATAGA957.620165E-716.8571191
CCTTTAG1304.7421054E-916.0142611
TATACTG1601.8189894E-1115.9973875
ACCGTCG2050.015.6093548
ATTATAC951.4326091E-515.1575053
CTAAGGC756.245217E-414.9329483
ACACCGT756.2517327E-414.9308966
CGTCGTA1951.8189894E-1214.76884910
TCTAGCG2500.014.71894628
CGTAACT1851.0913936E-1114.70232412
GTCGCGT1757.4578566E-1114.6281958
CGAACGA1103.5410867E-614.54574616
CCGTCGT2100.014.4758179
ATACCGT2003.6379788E-1214.3976516
Produced by FastQC (version 0.11.5)