##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062751_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1091401 Sequences flagged as poor quality 0 Sequence length 38 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21237656919867 32.0 32.0 32.0 32.0 32.0 2 30.86223028932537 32.0 32.0 32.0 32.0 32.0 3 30.91926981925067 32.0 32.0 32.0 32.0 32.0 4 30.965009194604 32.0 32.0 32.0 32.0 32.0 5 30.915404145680643 32.0 32.0 32.0 32.0 32.0 6 34.53558499579898 36.0 36.0 36.0 32.0 36.0 7 34.46823578134892 36.0 36.0 36.0 32.0 36.0 8 34.45363253286372 36.0 36.0 36.0 32.0 36.0 9 34.557609897736945 36.0 36.0 36.0 32.0 36.0 10 34.31048441406962 36.0 36.0 36.0 32.0 36.0 11 34.5613115619282 36.0 36.0 36.0 32.0 36.0 12 34.39412369972173 36.0 36.0 36.0 32.0 36.0 13 34.467000671613825 36.0 36.0 36.0 32.0 36.0 14 34.37867474924432 36.0 36.0 36.0 32.0 36.0 15 34.33572628209063 36.0 36.0 36.0 32.0 36.0 16 34.347116229506845 36.0 36.0 36.0 32.0 36.0 17 34.2884915810046 36.0 36.0 36.0 32.0 36.0 18 34.29183407381888 36.0 36.0 36.0 32.0 36.0 19 34.284043170200505 36.0 36.0 36.0 32.0 36.0 20 34.26279983250886 36.0 36.0 36.0 32.0 36.0 21 34.24128161876341 36.0 36.0 36.0 32.0 36.0 22 34.217120929887365 36.0 36.0 36.0 32.0 36.0 23 34.17288054528079 36.0 36.0 36.0 32.0 36.0 24 34.162372033743786 36.0 36.0 36.0 32.0 36.0 25 34.13536821021788 36.0 36.0 36.0 32.0 36.0 26 34.090239059704 36.0 36.0 36.0 32.0 36.0 27 34.08591525937763 36.0 36.0 36.0 32.0 36.0 28 34.072292402151 36.0 36.0 36.0 32.0 36.0 29 34.041203004212015 36.0 36.0 36.0 32.0 36.0 30 34.0129704847256 36.0 36.0 36.0 32.0 36.0 31 34.03039671028339 36.0 36.0 36.0 32.0 36.0 32 33.983523929334865 36.0 36.0 36.0 32.0 36.0 33 33.93812723279528 36.0 36.0 36.0 32.0 36.0 34 33.933495571288645 36.0 36.0 36.0 32.0 36.0 35 33.889392624708975 36.0 36.0 36.0 32.0 36.0 36 33.852324672599714 36.0 36.0 36.0 32.0 36.0 37 33.84371647084802 36.0 36.0 36.0 32.0 36.0 38 33.24415040851163 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 1.0 9 6.0 10 8.0 11 7.0 12 5.0 13 3.0 14 131.0 15 318.0 16 396.0 17 474.0 18 639.0 19 826.0 20 1091.0 21 1532.0 22 2211.0 23 3460.0 24 4679.0 25 7221.0 26 10586.0 27 15211.0 28 21053.0 29 28582.0 30 38670.0 31 51622.0 32 69941.0 33 103371.0 34 230679.0 35 498676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29857372976821 17.400900454630516 11.746731540496608 25.553794275104668 2 16.474360972425693 20.042299307479478 37.662793661154495 25.820546058940337 3 18.828056201839857 24.62088133120617 28.479557510851926 28.07150495610205 4 12.701718570753142 15.864159974784034 36.0546174906448 35.379503963818024 5 14.712323093866766 36.408532909167874 33.448567800197544 15.430576196767815 6 33.5800204508721 35.48290446364106 17.337978149086126 13.59909693640072 7 29.795831229767977 30.79363130508402 21.30949119526187 18.101046269886137 8 27.93909468489526 32.39411067812963 19.996444929856082 19.67034970711903 9 27.748355778551915 14.64946793012265 18.71553732002441 38.88663897130103 10 16.033853858861626 26.662616811297813 31.544525044507175 25.759004285333386 11 36.74934486613279 21.450823727757516 22.45184995143763 19.347981454672066 12 25.268353648647533 23.832003056688936 28.5866387142115 22.313004580452034 13 29.43622619862527 19.83357021308181 25.19584961022514 25.534353978067774 14 24.1445190943778 19.860242129685627 25.38439828610595 30.610840489830625 15 25.294524659423644 26.600468306809695 22.96096374058919 25.144043293177475 16 25.58735156423961 25.81332121319861 23.963687245543603 24.635639977018172 17 24.057430720321705 25.713508059527506 25.51235207703862 24.716709143112173 18 24.99280710017098 24.752005307168893 26.40398990611662 23.851197686543504 19 25.421753317261302 25.18525441728815 25.87175180535435 23.521240460096195 20 25.732690096256487 24.121482010894713 25.551630770922383 24.594197121926413 21 26.896267985899915 24.404379566056804 24.686509532767484 24.012842915275794 22 25.754074356432195 24.377232351480476 25.086728401283565 24.781964890803764 23 24.24877739913371 24.244104163669533 25.729734880937873 25.777383556258883 24 24.61973100991783 25.261645408502226 25.329456494148545 24.789167087431398 25 24.97392878939777 24.512894481700666 25.282476297649836 25.23070043125173 26 24.613891564310777 25.361777624634698 25.663369711943357 24.36096109911117 27 25.421619734506706 24.883375926738733 24.968410018262173 24.726594320492392 28 24.442021349704497 24.680533284464197 25.898657625876208 24.978787739955102 29 24.42556778872807 25.077728753453464 25.92305471939301 24.57364873842545 30 24.438529378078115 25.119688466384147 26.139411293093573 24.302370862444164 31 24.87952960882286 25.108695950967014 24.874215075498444 25.13755936471168 32 24.446987696787716 25.071448795564354 24.94710635814104 25.53445714950689 33 24.23600172633234 24.733101139800848 25.48053706895525 25.550360064911565 34 24.850222032435244 24.885868180726888 25.608871034251457 24.655038752586407 35 25.58921232170594 24.79324074795303 25.432510572786132 24.185036357554903 36 24.53569513999936 25.549482018409996 25.078373065873695 24.83644977571695 37 25.358902533003825 25.39922093844754 25.06494470422326 24.176931824325376 38 24.35872766290434 25.396162170384095 25.401751598484807 24.84335856822676 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 568.0 1 435.5 2 303.0 3 303.0 4 696.5 5 1090.0 6 1090.0 7 1218.0 8 1346.0 9 1312.0 10 1278.0 11 1278.0 12 1622.5 13 1967.0 14 2458.5 15 2950.0 16 2950.0 17 4192.5 18 5435.0 19 5435.0 20 6622.5 21 7810.0 22 8229.0 23 8648.0 24 8648.0 25 9783.0 26 10918.0 27 10918.0 28 13935.0 29 16952.0 30 20918.5 31 24885.0 32 24885.0 33 31037.0 34 37189.0 35 37189.0 36 41584.5 37 45980.0 38 52150.5 39 58321.0 40 58321.0 41 63861.0 42 69401.0 43 78460.0 44 87519.0 45 87519.0 46 92328.5 47 97138.0 48 97138.0 49 100456.0 50 103774.0 51 102939.0 52 102104.0 53 102104.0 54 97049.0 55 91994.0 56 91994.0 57 88650.0 58 85306.0 59 76415.0 60 67524.0 61 67524.0 62 62947.0 63 58370.0 64 48024.5 65 37679.0 66 37679.0 67 31461.0 68 25243.0 69 25243.0 70 20178.0 71 15113.0 72 11968.0 73 8823.0 74 8823.0 75 6621.0 76 4419.0 77 4419.0 78 4278.5 79 4138.0 80 3235.5 81 2333.0 82 2333.0 83 2399.0 84 2465.0 85 2465.0 86 1609.0 87 753.0 88 679.0 89 605.0 90 605.0 91 425.5 92 246.0 93 216.0 94 186.0 95 186.0 96 155.0 97 124.0 98 124.0 99 314.0 100 504.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11773857638026719 2 0.055250086815020326 3 0.01099504215224285 4 0.0026571351867920225 5 2.7487605380607127E-4 6 4.581267563434521E-4 7 0.0 8 1.8325070253738085E-4 9 2.7487605380607127E-4 10 3.665014050747617E-4 11 0.001924132376642499 12 0.00219900843044857 13 0.011911295664929756 14 0.007788154857838687 15 0.02162358289941094 16 0.009529036531943804 17 0.01905807306388761 18 0.00430639150962845 19 0.005864022481196187 20 0.00421476615835976 21 0.005497521076121425 22 0.005772397129927497 23 0.007330028101495234 24 0.012277797070004516 25 0.014568430851721778 26 0.01759206744358856 27 0.006780275993883091 28 0.004672892914703212 29 0.009712287234481184 30 0.002382259132985951 31 0.004947768968509283 32 0.005497521076121425 33 0.00641377458880833 34 0.010903416800974161 35 0.014293554797915707 36 0.013377301285228804 37 0.008063030911644757 38 0.005039394319777974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 1091401.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.14749427994667 #Duplication Level Percentage of deduplicated Percentage of total 1 83.33276589260457 51.789225916427775 2 10.941719923202914 13.600009526800633 3 2.783856234679271 5.190290682627715 4 1.064591050313333 2.646466648393211 5 0.5230499090487382 1.6253120615366534 6 0.30608695746167014 1.1413522462809256 7 0.19663848479497037 0.8554412376308961 8 0.12663358575916306 0.6295968037293377 9 0.09344355899660958 0.5226554743415688 >10 0.4920897249150495 5.752596056330853 >50 0.06107555349008623 2.699117167885621 >100 0.06977118898648238 9.137880006461316 >500 0.0063562720915822735 2.718608317566579 >1k 0.0019216636555946412 1.6914478539869513 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 3401 0.31161781966481616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2160 0.19791075874037134 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1547 0.14174441841266408 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 1340 0.12277797070004517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1225 0.11224105530414578 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 1169 0.10711003563309912 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 1163 0.10656028352548698 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 1138 0.1042696497437697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7487605380607127E-4 2 0.0 0.0 0.0 0.0 2.7487605380607127E-4 3 0.0 0.0 0.0 0.0 2.7487605380607127E-4 4 0.0 0.0 0.0 0.0 2.7487605380607127E-4 5 0.0 0.0 0.0 0.0 2.7487605380607127E-4 6 0.0 0.0 0.0 0.0 2.7487605380607127E-4 7 0.0 0.0 0.0 0.0 2.7487605380607127E-4 8 0.0 0.0 0.0 9.162535126869042E-5 2.7487605380607127E-4 9 0.0 0.0 0.0 9.162535126869042E-5 2.7487605380607127E-4 10 0.0 0.0 0.0 9.162535126869042E-5 2.7487605380607127E-4 11 0.0 0.0 0.0 9.162535126869042E-5 2.7487605380607127E-4 12 0.0 0.0 0.0 9.162535126869042E-5 3.665014050747617E-4 13 0.0 0.0 0.0 1.8325070253738085E-4 3.665014050747617E-4 14 0.0 0.0 0.0 1.8325070253738085E-4 3.665014050747617E-4 15 0.0 0.0 0.0 1.8325070253738085E-4 4.581267563434521E-4 16 0.0 0.0 0.0 1.8325070253738085E-4 4.581267563434521E-4 17 0.0 0.0 0.0 1.8325070253738085E-4 4.581267563434521E-4 18 0.0 0.0 0.0 1.8325070253738085E-4 4.581267563434521E-4 19 0.0 0.0 0.0 1.8325070253738085E-4 4.581267563434521E-4 20 0.0 0.0 0.0 1.8325070253738085E-4 5.497521076121425E-4 21 0.0 0.0 0.0 5.497521076121425E-4 5.497521076121425E-4 22 0.0 0.0 0.0 0.001099504215224285 6.41377458880833E-4 23 9.162535126869042E-5 0.0 0.0 0.0018325070253738085 6.41377458880833E-4 24 9.162535126869042E-5 0.0 0.0 0.003390137996941546 6.41377458880833E-4 25 9.162535126869042E-5 0.0 0.0 0.00430639150962845 6.41377458880833E-4 26 9.162535126869042E-5 0.0 0.0 0.005405895724852735 8.246281614182139E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACT 165 0.0 18.421236 4 TAACGCA 45 0.008858319 17.774878 4 CGCGTAA 145 3.6379788E-12 17.654716 10 TCGCGTA 145 3.6379788E-12 17.654716 9 GCGTAAC 150 5.456968E-12 17.066227 11 CTATATA 95 7.620165E-7 16.857119 1 GTATAGA 95 7.620165E-7 16.857119 1 CCTTTAG 130 4.7421054E-9 16.014261 1 TATACTG 160 1.8189894E-11 15.997387 5 ACCGTCG 205 0.0 15.609354 8 ATTATAC 95 1.4326091E-5 15.157505 3 CTAAGGC 75 6.245217E-4 14.932948 3 ACACCGT 75 6.2517327E-4 14.930896 6 CGTCGTA 195 1.8189894E-12 14.768849 10 TCTAGCG 250 0.0 14.718946 28 CGTAACT 185 1.0913936E-11 14.702324 12 GTCGCGT 175 7.4578566E-11 14.628195 8 CGAACGA 110 3.5410867E-6 14.545746 16 CCGTCGT 210 0.0 14.475817 9 ATACCGT 200 3.6379788E-12 14.397651 6 >>END_MODULE