FastQCFastQC Report
Thu 2 Feb 2017
SRR4062751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091401
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39310.3601792558372221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26240.24042492172904367No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16860.15448034223901208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15700.14385180149184398No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14810.1356971452289305No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.1166390721650429No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12580.11526469189601257No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11840.10848441590212946No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11310.10362827228488887No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11310.10362827228488887No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11140.10207064131332114No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11080.101520889205709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG701.7860248E-518.2870245
TACCGTC2900.018.20827
ACCGTCG2850.017.9661988
TAATACC450.00884868317.7782364
CTATCCT1101.0626536E-817.4549944
ATACCGT2950.017.3571746
CCGTCGT3000.016.5337589
TCTAGCG2250.016.35522828
AGCTATA600.00244063316.0011467
CGTCGTA3100.016.00041210
CTAGCGG2300.015.30404129
CGTTTAT2200.015.27242231
GTATTAG2000.015.1996961
GCATCGT2550.015.05852327
GTCGTAG3300.015.0306911
TTAAGAC1603.0377123E-1015.0003873
TAGCGGC2400.014.66637330
CGCGTAA1553.1286618E-914.45198510
AGCGGCG2550.014.43108531
ATCGTTT2450.014.3670629
Produced by FastQC (version 0.11.5)