##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091401
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161011397277445	32.0	32.0	32.0	32.0	32.0
2	31.234061541083435	32.0	32.0	32.0	32.0	32.0
3	31.33354559873044	32.0	32.0	32.0	32.0	32.0
4	31.443804797686642	32.0	32.0	32.0	32.0	32.0
5	31.346834023424936	32.0	32.0	32.0	32.0	32.0
6	34.853920786218815	36.0	36.0	36.0	32.0	36.0
7	34.88699020799871	36.0	36.0	36.0	32.0	36.0
8	34.82988287531347	36.0	36.0	36.0	32.0	36.0
9	34.94443838699067	36.0	36.0	36.0	32.0	36.0
10	34.77319060546948	36.0	36.0	36.0	32.0	36.0
11	34.968803400400034	36.0	36.0	36.0	36.0	36.0
12	34.859608888025576	36.0	36.0	36.0	32.0	36.0
13	34.920731243603406	36.0	36.0	36.0	32.0	36.0
14	34.86054804787608	36.0	36.0	36.0	32.0	36.0
15	34.83072674479866	36.0	36.0	36.0	32.0	36.0
16	34.84517239767968	36.0	36.0	36.0	32.0	36.0
17	34.799067437174784	36.0	36.0	36.0	32.0	36.0
18	34.79991039040646	36.0	36.0	36.0	32.0	36.0
19	34.801311342027354	36.0	36.0	36.0	32.0	36.0
20	34.77971982800089	36.0	36.0	36.0	32.0	36.0
21	34.77078727250571	36.0	36.0	36.0	32.0	36.0
22	34.757149755222876	36.0	36.0	36.0	32.0	36.0
23	34.687236863444326	36.0	36.0	36.0	32.0	36.0
24	34.662149842266956	36.0	36.0	36.0	32.0	36.0
25	34.65165599078615	36.0	36.0	36.0	32.0	36.0
26	34.58215266432778	36.0	36.0	36.0	32.0	36.0
27	34.56877627929606	36.0	36.0	36.0	32.0	36.0
28	34.53371400612607	36.0	36.0	36.0	32.0	36.0
29	34.49494457124375	36.0	36.0	36.0	32.0	36.0
30	34.471349210785036	36.0	36.0	36.0	32.0	36.0
31	34.46331824874633	36.0	36.0	36.0	32.0	36.0
32	34.4206767265194	36.0	36.0	36.0	32.0	36.0
33	34.39109181684825	36.0	36.0	36.0	32.0	36.0
34	34.36487139007569	36.0	36.0	36.0	32.0	36.0
35	34.33094618751495	36.0	36.0	36.0	32.0	36.0
36	34.298263424717405	36.0	36.0	36.0	32.0	36.0
37	34.286329222714656	36.0	36.0	36.0	32.0	36.0
38	33.85051690441918	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	18.0
22	88.0
23	293.0
24	729.0
25	1872.0
26	4058.0
27	7787.0
28	13761.0
29	21617.0
30	32510.0
31	47245.0
32	66685.0
33	106495.0
34	250845.0
35	537393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64898987353604	18.638338347235724	12.478340033299764	26.234331745928475
2	15.53481766538391	21.228147333699834	36.79228513835441	26.444749862561846
3	17.642919513542683	25.451781700768095	28.9005599225216	28.004738863167617
4	12.259780575005635	16.858561221479242	35.8836098288617	34.99804837465343
5	14.270569910207072	36.95391240608393	33.06239692138538	15.713120762323621
6	33.453668181847334	35.97932561785378	16.967305265993463	13.59970093430542
7	29.648395910566084	30.900917905292115	21.271525144814266	18.17916103932754
8	27.94999299044226	32.64529713652694	19.70803588527049	19.696673987760306
9	27.675225359778477	14.377944704159743	18.581025980285258	39.36580395577652
10	15.752591866372853	26.949821100518147	31.535511890745333	25.76207514236367
11	36.99064817682515	21.313103731382945	22.413679355066897	19.28256873672501
12	24.98824900563223	23.961858058983122	28.6012330984967	22.448659836887952
13	29.555304297790542	19.83162879169652	25.173859581930696	25.439207328582242
14	23.961586987734115	19.750760719478908	25.412566050425095	30.87508624236188
15	25.276502403791092	26.666458982537122	22.91797423678373	25.139064376888054
16	25.650700338372424	25.820115612868232	23.80234212722913	24.726841921530216
17	24.065123634667735	25.887826747455794	25.34439678908119	24.702652828795284
18	25.000710096472332	24.653816516569073	26.566953851059328	23.77851953589927
19	25.472122528749747	25.14914316552761	25.82423875367532	23.55449555204732
20	25.74883108958119	23.99686274797256	25.641354552543017	24.612951609903234
21	26.7575345816982	24.45874614371803	24.800875205355318	23.982844069228452
22	25.60995052226498	24.357247571925967	25.194245922668134	24.83855598314092
23	24.114920391167665	24.262712353593873	25.800829943952667	25.82153731128579
24	24.58793788900688	25.185793306035087	25.344671665134992	24.881597139823032
25	24.923469925352826	24.45929589582564	25.400013377301285	25.217220801520245
26	24.66316230239848	25.20860801850099	25.80618855947539	24.322041119625144
27	25.34167124793843	24.750503939893715	25.02620487447315	24.881619937694705
28	24.519951878365514	24.52462477128022	26.032228301055248	24.92319504929902
29	24.531038545869023	24.913757638118344	26.05046174595772	24.50474207005491
30	24.518577498096484	25.01124701186823	26.13503194517872	24.335143544856567
31	24.885078903171244	24.88324639614587	25.01701940899816	25.214655291684725
32	24.59444328894696	24.919255159194467	25.08637979990856	25.399921751950018
33	24.27806095101617	24.506391326377745	25.702926788595576	25.512620934010506
34	24.925210807026932	24.631368305508243	25.893965645990797	24.549455241474032
35	25.655648107340934	24.510789343238645	25.555959725160598	24.277602824259827
36	24.686984893728336	25.23820300696078	25.26963050244594	24.805181596864948
37	25.46763288653758	25.11771567004245	25.150884047201714	24.263767396218256
38	24.535550668865678	24.938794209272494	25.61077515118197	24.91487997067986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	297.0
1	258.0
2	219.0
3	219.0
4	597.0
5	975.0
6	975.0
7	1158.0
8	1341.0
9	1343.0
10	1345.0
11	1345.0
12	1714.0
13	2083.0
14	2552.5
15	3022.0
16	3022.0
17	4432.0
18	5842.0
19	5842.0
20	6971.5
21	8101.0
22	8363.5
23	8626.0
24	8626.0
25	9810.5
26	10995.0
27	10995.0
28	13902.5
29	16810.0
30	20612.5
31	24415.0
32	24415.0
33	30951.0
34	37487.0
35	37487.0
36	41709.0
37	45931.0
38	52325.0
39	58719.0
40	58719.0
41	63983.0
42	69247.0
43	78220.0
44	87193.0
45	87193.0
46	92916.0
47	98639.0
48	98639.0
49	102175.5
50	105712.0
51	103627.0
52	101542.0
53	101542.0
54	96475.5
55	91409.0
56	91409.0
57	88235.0
58	85061.0
59	76170.5
60	67280.0
61	67280.0
62	62958.0
63	58636.0
64	48097.5
65	37559.0
66	37559.0
67	31314.0
68	25069.0
69	25069.0
70	20205.0
71	15341.0
72	11967.0
73	8593.0
74	8593.0
75	6486.5
76	4380.0
77	4380.0
78	4249.0
79	4118.0
80	3206.5
81	2295.0
82	2295.0
83	2182.5
84	2070.0
85	2070.0
86	1313.0
87	556.0
88	460.0
89	364.0
90	364.0
91	217.5
92	71.0
93	52.5
94	34.0
95	34.0
96	24.0
97	14.0
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009345785829406424
2	9.162535126869042E-5
3	0.0
4	2.7487605380607127E-4
5	9.162535126869042E-5
6	1.8325070253738085E-4
7	2.7487605380607127E-4
8	0.0031152619431354745
9	0.003206887294404165
10	5.497521076121425E-4
11	0.005772397129927497
12	3.665014050747617E-4
13	2.7487605380607127E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	9.162535126869042E-5
23	1.8325070253738085E-4
24	0.0
25	0.0
26	0.0
27	9.162535126869042E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.162535126869042E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1091401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.37595598136297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09606725919654	49.56628543084264
2	11.804798803986518	14.254520259166725
3	2.9900809245372115	5.415869828417153
4	1.0836790270642882	2.6171262894383887
5	0.5684056523368896	1.7159017322524979
6	0.3300545235209098	1.1956414430128908
7	0.21268714413923726	0.8988832756646673
8	0.13647951225434565	0.6592064819381009
9	0.10803628768545996	0.5870514734718476
>10	0.5189638301739445	5.8192442932674115
>50	0.06655624427478284	2.8186422658490025
>100	0.07414270531985692	9.324315728651575
>500	0.007612185992455764	2.9584001822126154
>1k	0.0024358995175858445	2.168911315814475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3931	0.3601792558372221	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2624	0.24042492172904367	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1686	0.15448034223901208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1570	0.14385180149184398	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1481	0.1356971452289305	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.1166390721650429	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1258	0.11526469189601257	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1184	0.10848441590212946	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1131	0.10362827228488887	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1131	0.10362827228488887	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1114	0.10207064131332114	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1108	0.101520889205709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.162535126869042E-5
2	0.0	0.0	0.0	0.0	9.162535126869042E-5
3	0.0	0.0	0.0	0.0	9.162535126869042E-5
4	0.0	0.0	0.0	0.0	9.162535126869042E-5
5	0.0	0.0	0.0	0.0	9.162535126869042E-5
6	0.0	0.0	0.0	0.0	9.162535126869042E-5
7	0.0	0.0	0.0	0.0	9.162535126869042E-5
8	0.0	0.0	0.0	0.0	9.162535126869042E-5
9	0.0	0.0	0.0	0.0	9.162535126869042E-5
10	0.0	0.0	0.0	0.0	9.162535126869042E-5
11	0.0	0.0	0.0	0.0	9.162535126869042E-5
12	0.0	0.0	0.0	0.0	9.162535126869042E-5
13	0.0	0.0	0.0	0.0	9.162535126869042E-5
14	0.0	0.0	0.0	0.0	9.162535126869042E-5
15	0.0	0.0	0.0	0.0	9.162535126869042E-5
16	0.0	0.0	0.0	0.0	9.162535126869042E-5
17	0.0	0.0	0.0	0.0	9.162535126869042E-5
18	0.0	0.0	0.0	0.0	9.162535126869042E-5
19	0.0	0.0	0.0	0.0	9.162535126869042E-5
20	0.0	0.0	0.0	0.0	9.162535126869042E-5
21	0.0	0.0	0.0	3.665014050747617E-4	9.162535126869042E-5
22	0.0	0.0	0.0	8.246281614182139E-4	9.162535126869042E-5
23	0.0	0.0	0.0	0.0016492563228364277	9.162535126869042E-5
24	0.0	0.0	0.0	0.003023636591866784	9.162535126869042E-5
25	0.0	0.0	0.0	0.003939890104553688	9.162535126869042E-5
26	0.0	0.0	0.0	0.005039394319777973	9.162535126869042E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	70	1.7860248E-5	18.287024	5
TACCGTC	290	0.0	18.2082	7
ACCGTCG	285	0.0	17.966198	8
TAATACC	45	0.008848683	17.778236	4
CTATCCT	110	1.0626536E-8	17.454994	4
ATACCGT	295	0.0	17.357174	6
CCGTCGT	300	0.0	16.533758	9
TCTAGCG	225	0.0	16.355228	28
AGCTATA	60	0.002440633	16.001146	7
CGTCGTA	310	0.0	16.000412	10
CTAGCGG	230	0.0	15.304041	29
CGTTTAT	220	0.0	15.272422	31
GTATTAG	200	0.0	15.199696	1
GCATCGT	255	0.0	15.058523	27
GTCGTAG	330	0.0	15.03069	11
TTAAGAC	160	3.0377123E-10	15.000387	3
TAGCGGC	240	0.0	14.666373	30
CGCGTAA	155	3.1286618E-9	14.451985	10
AGCGGCG	255	0.0	14.431085	31
ATCGTTT	245	0.0	14.36706	29
>>END_MODULE