FastQCFastQC Report
Thu 2 Feb 2017
SRR4062750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567042
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA166640.6491518253304777No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA160430.6249605577158457No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG146150.5693323288049047No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC134680.5246505511012286No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA88400.34436522659154No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA78930.3074745173627857No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT61740.24051028382083348No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA60890.23719907971899173No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA59070.23010920740681298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55770.21725394442319215No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA54590.21265721402298834No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA52130.2030741997988346No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT40770.15882093086127924No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG39140.15247121005421804No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT38460.14982224677274467No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG37990.14799134568113806No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA35840.1396159470705972No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC35740.1392263936468511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34620.13486339530089497No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA33140.1290980046294529No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG33130.12905904928707826No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT32830.12789038901584002No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT32450.12641008600560488No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT30760.11982663314429605No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA30590.1191643923239277No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT28610.1114512345337552No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA27540.10728301289967207No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT26910.10482882633007172No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG26420.1029200145537159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC350.002068701422.8543478
TAGGACC42300.020.307994
CGCGAGA400.00448560919.999519
ACCGTGC400.00448864419.9971647
CTGACCG400.004490162219.9959934
TAGGGCC802.8042687E-617.9963934
CGACAAC450.00885443717.77733419
GTATCAA55150.017.6275731
GTACCGC550.001367100617.451056
AGTACGT1750.017.3679495
AGTACCG652.1048929E-417.227325
AGGACCT84750.017.138755
GTCTTAC4600.017.0603351
GGACGTC1601.8189894E-1216.9965936
TCTTCGC957.70664E-716.84102829
TACGTGG957.7129334E-716.8397167
GTCCTAT9800.016.832961
TAGAAAT47050.016.7617864
ATGTCCA46800.016.7520459
AATGTCC48150.016.6127138
Produced by FastQC (version 0.11.5)