##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062750_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567042
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332975853141477	32.0	32.0	32.0	32.0	32.0
2	31.054996373257627	32.0	32.0	32.0	32.0	32.0
3	31.084897714957528	32.0	32.0	32.0	32.0	32.0
4	31.14115935773548	32.0	32.0	32.0	32.0	32.0
5	31.125108977570292	32.0	32.0	32.0	32.0	32.0
6	34.79626394893422	36.0	36.0	36.0	32.0	36.0
7	34.719677356272314	36.0	36.0	36.0	32.0	36.0
8	34.70907799716561	36.0	36.0	36.0	32.0	36.0
9	34.79529512956936	36.0	36.0	36.0	32.0	36.0
10	34.58696117944311	36.0	36.0	36.0	32.0	36.0
11	34.770738071289834	36.0	36.0	36.0	32.0	36.0
12	34.64150372296207	36.0	36.0	36.0	32.0	36.0
13	34.694430398879334	36.0	36.0	36.0	32.0	36.0
14	34.64164318308777	36.0	36.0	36.0	32.0	36.0
15	34.6126019753475	36.0	36.0	36.0	32.0	36.0
16	34.652500426561	36.0	36.0	36.0	32.0	36.0
17	34.61386373888701	36.0	36.0	36.0	32.0	36.0
18	34.65014713432815	36.0	36.0	36.0	32.0	36.0
19	34.54761005078998	36.0	36.0	36.0	32.0	36.0
20	34.52465873172313	36.0	36.0	36.0	32.0	36.0
21	34.488525314350134	36.0	36.0	36.0	32.0	36.0
22	34.460259707476546	36.0	36.0	36.0	32.0	36.0
23	34.46587512007984	36.0	36.0	36.0	32.0	36.0
24	34.41711783445694	36.0	36.0	36.0	32.0	36.0
25	34.38875678699453	36.0	36.0	36.0	32.0	36.0
26	34.39873402928351	36.0	36.0	36.0	32.0	36.0
27	34.33168175666779	36.0	36.0	36.0	32.0	36.0
28	34.24696440494546	36.0	36.0	36.0	32.0	36.0
29	34.18136477704689	36.0	36.0	36.0	32.0	36.0
30	34.12091738273079	36.0	36.0	36.0	32.0	36.0
31	34.11427354908879	36.0	36.0	36.0	32.0	36.0
32	34.08229199210609	36.0	36.0	36.0	32.0	36.0
33	34.13077931720634	36.0	36.0	36.0	32.0	36.0
34	34.078409702685036	36.0	36.0	36.0	32.0	36.0
35	34.08337261330356	36.0	36.0	36.0	32.0	36.0
36	33.98673882234883	36.0	36.0	36.0	32.0	36.0
37	33.893232366279946	36.0	36.0	36.0	32.0	36.0
38	33.3138382620931	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	4.0
9	17.0
10	29.0
11	24.0
12	12.0
13	15.0
14	157.0
15	426.0
16	628.0
17	758.0
18	943.0
19	1263.0
20	1656.0
21	2344.0
22	3367.0
23	5191.0
24	7847.0
25	12392.0
26	19047.0
27	28181.0
28	41552.0
29	58611.0
30	81040.0
31	110992.0
32	154480.0
33	236968.0
34	570251.0
35	1228841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32227637507332	21.634978408197398	13.870159005528999	25.172586211200283
2	11.11064772355667	20.97342307807738	49.45080129885366	18.465127899512286
3	17.686703784898665	27.819427891970903	31.37278888930879	23.12107943382164
4	10.384510652023762	18.56074285044237	40.962891369887615	30.091855127646255
5	9.756902323849236	40.99844411877677	37.10309251844736	12.141561038926636
6	27.424667865457074	39.97772524845814	20.56522863758613	12.032378248498656
7	25.313464023363863	32.85319556641284	24.367238388479187	17.4661020217441
8	21.541695445049164	44.382206525749005	20.78556900948995	13.290529019711878
9	26.63312080522533	15.760218119517747	22.63866494898975	34.967996126267174
10	16.024113516619433	29.28522316689553	35.468840389166445	19.221822927318588
11	31.83181299708295	20.189281084045973	31.47898538981276	16.499920529058315
12	26.83171020651284	23.419735180811223	34.816566996200564	14.93198761647537
13	29.591824410275592	24.180755961529847	27.300134610477016	18.927285017717544
14	18.00974215584255	27.35992875128127	27.793584420654184	26.836744672222
15	18.231544543367797	42.23481136482922	22.4622030237581	17.071441068044884
16	16.65897207232373	28.46633683154916	36.50451916187751	18.370171934249594
17	17.382966072571048	33.34336004438768	31.981220957280755	17.292452925760518
18	18.036622005304512	26.510048027326643	40.06756082889992	15.385769138468921
19	24.171677417997564	25.27327652101009	28.774465154078165	21.780580906914174
20	24.09694738002639	29.273003731772114	30.63259361473105	15.997455273470445
21	20.523245281695765	27.112801023816992	27.5185488913519	24.84540480313534
22	22.96605943494725	33.955564039831074	27.801070577051938	15.277305948169733
23	16.59169606792001	33.543882149803025	32.10363630130155	17.760785480975414
24	23.12123098789319	27.765370790141564	32.905175376190165	16.208222845775083
25	19.515441347503664	30.61424469810639	32.95299626000796	16.917317694381985
26	15.368319913221896	33.07359384947708	33.98836412828759	17.569722109013437
27	17.58016866870006	32.18500650808573	31.395387343107608	18.839437480106607
28	16.157198945638683	33.22087435943187	29.08948747536876	21.532439219560693
29	20.147314181035306	29.56405334509395	27.851426643483084	22.43720583038766
30	16.52568866775304	30.353076745973528	31.59256377902327	21.528670807250165
31	22.456472245553723	27.380215889558794	30.275651104770485	19.887660760116997
32	18.31296978933647	32.079302115206154	30.800181701873353	18.807546393584023
33	15.805963619249983	32.8103858622244	34.91187655276206	16.471773965763557
34	18.603138957545685	31.623415482531737	33.60766615031215	16.16577940961043
35	17.17784718196156	33.00628689694726	32.62348593941887	17.19237998167231
36	15.850423783953952	31.420013262485792	34.234745998461804	18.494816955098457
37	17.195016142875456	31.70272175074715	28.59414196925549	22.508120137121914
38	18.731546126356854	35.13033173047517	28.49120310977598	17.646919033391992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	523.0
1	435.5
2	348.0
3	348.0
4	804.0
5	1260.0
6	1260.0
7	1503.0
8	1746.0
9	1706.0
10	1666.0
11	1666.0
12	2275.5
13	2885.0
14	3995.0
15	5105.0
16	5105.0
17	8103.5
18	11102.0
19	11102.0
20	15319.5
21	19537.0
22	26980.0
23	34423.0
24	34423.0
25	53598.0
26	72773.0
27	72773.0
28	104839.0
29	136905.0
30	195527.5
31	254150.0
32	254150.0
33	319070.0
34	383990.0
35	383990.0
36	391574.0
37	399158.0
38	388882.5
39	378607.0
40	378607.0
41	320403.5
42	262200.0
43	212257.0
44	162314.0
45	162314.0
46	134074.5
47	105835.0
48	105835.0
49	93536.0
50	81237.0
51	71117.5
52	60998.0
53	60998.0
54	51787.0
55	42576.0
56	42576.0
57	40136.5
58	37697.0
59	31877.5
60	26058.0
61	26058.0
62	26982.0
63	27906.0
64	22659.5
65	17413.0
66	17413.0
67	14990.5
68	12568.0
69	12568.0
70	10518.0
71	8468.0
72	6627.0
73	4786.0
74	4786.0
75	3624.5
76	2463.0
77	2463.0
78	2863.5
79	3264.0
80	2579.5
81	1895.0
82	1895.0
83	2130.5
84	2366.0
85	2366.0
86	1540.0
87	714.0
88	645.5
89	577.0
90	577.0
91	421.0
92	265.0
93	226.5
94	188.0
95	188.0
96	190.5
97	193.0
98	193.0
99	538.0
100	883.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12099529341553429
2	0.05434270261257899
3	0.011258093946261885
4	0.002532097254349559
5	3.116427389968688E-4
6	3.8955342374608597E-4
7	3.89553423746086E-5
8	4.285087661206945E-4
9	3.5059808137147735E-4
10	6.622408203683461E-4
11	0.0025710525967241674
12	0.0032332934170925132
13	0.01468616407522744
14	0.010089433675023627
15	0.024658731723127242
16	0.011881379424255621
17	0.021970813099279247
18	0.0066224082036834605
19	0.00888181806141076
20	0.006427631491810418
21	0.006895095600305721
22	0.007868979159670938
23	0.010050478332649018
24	0.015114672841348136
25	0.017880502149945347
26	0.019321849817805863
27	0.00915450545803302
28	0.007011961627429546
29	0.011024361892014233
30	0.003622846840838599
31	0.006778229573181895
32	0.00779106847492172
33	0.009544058881779106
34	0.012855262983620837
35	0.01690661859058013
36	0.015737958319341876
37	0.010712719153017365
38	0.006583452861308853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2567042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12438225051136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93583198969262	39.08299848946463
2	10.198953282788253	9.612387461064428
3	2.637713312353076	3.729018311957665
4	1.1206447967030952	2.11238775067533
5	0.6123967366218396	1.442940895276665
6	0.3928593401575668	1.1107952229761306
7	0.2998014496893214	0.988957068009192
8	0.21815460615823173	0.8224320840248217
9	0.1714513253570675	0.7271584014134915
>10	1.1443797379048295	10.463796448253879
>50	0.13628669195638607	4.4864211010960044
>100	0.11009493704415817	10.499190693705396
>500	0.013156969800115452	4.2261953043365486
>1k	0.007281844920818589	6.3301877183723025
>5k	6.61985901892599E-4	1.994651567254012
>10k+	3.309929509462995E-4	2.3704814821193967
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	16664	0.6491518253304777	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	16043	0.6249605577158457	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	14615	0.5693323288049047	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	13468	0.5246505511012286	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	8840	0.34436522659154	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	7893	0.3074745173627857	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	6174	0.24051028382083348	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6089	0.23719907971899173	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	5907	0.23010920740681298	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5577	0.21725394442319215	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	5459	0.21265721402298834	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5213	0.2030741997988346	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	4077	0.15882093086127924	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3914	0.15247121005421804	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	3846	0.14982224677274467	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	3799	0.14799134568113806	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	3584	0.1396159470705972	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	3574	0.1392263936468511	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3462	0.13486339530089497	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	3314	0.1290980046294529	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3313	0.12905904928707826	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3283	0.12789038901584002	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	3245	0.12641008600560488	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	3076	0.11982663314429605	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3059	0.1191643923239277	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2861	0.1114512345337552	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	2754	0.10728301289967207	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	2691	0.10482882633007172	No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2642	0.1029200145537159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.79106847492172E-5	0.0	0.0	0.0	0.0
8	7.79106847492172E-5	0.0	0.0	0.0	0.0
9	7.79106847492172E-5	0.0	0.0	0.0	0.0
10	7.79106847492172E-5	0.0	0.0	0.0	0.0
11	7.79106847492172E-5	0.0	0.0	0.0	0.0
12	7.79106847492172E-5	0.0	0.0	0.0	0.0
13	7.79106847492172E-5	0.0	0.0	0.0	0.0
14	7.79106847492172E-5	0.0	0.0	0.0	0.0
15	7.79106847492172E-5	0.0	0.0	3.89553423746086E-5	0.0
16	7.79106847492172E-5	0.0	0.0	7.79106847492172E-5	0.0
17	1.1686602712382578E-4	0.0	0.0	7.79106847492172E-5	0.0
18	1.1686602712382578E-4	0.0	0.0	7.79106847492172E-5	3.89553423746086E-5
19	1.1686602712382578E-4	0.0	0.0	7.79106847492172E-5	3.89553423746086E-5
20	1.1686602712382578E-4	0.0	0.0	1.9477671187304298E-4	3.89553423746086E-5
21	1.1686602712382578E-4	0.0	0.0	2.3373205424765157E-4	3.89553423746086E-5
22	1.1686602712382578E-4	0.0	0.0	4.285087661206946E-4	3.89553423746086E-5
23	1.1686602712382578E-4	0.0	0.0	9.738835593652149E-4	3.89553423746086E-5
24	1.558213694984344E-4	0.0	0.0	0.0018698564339812125	3.89553423746086E-5
25	1.558213694984344E-4	0.0	0.0	0.002103588488228864	3.89553423746086E-5
26	1.9477671187304298E-4	0.0	0.0	0.0027658293085972103	3.89553423746086E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTTC	35	0.0020687014	22.854347	8
TAGGACC	4230	0.0	20.30799	4
CGCGAGA	40	0.004485609	19.9995	19
ACCGTGC	40	0.004488644	19.997164	7
CTGACCG	40	0.0044901622	19.995993	4
TAGGGCC	80	2.8042687E-6	17.996393	4
CGACAAC	45	0.008854437	17.777334	19
GTATCAA	5515	0.0	17.627573	1
GTACCGC	55	0.0013671006	17.45105	6
AGTACGT	175	0.0	17.367949	5
AGTACCG	65	2.1048929E-4	17.22732	5
AGGACCT	8475	0.0	17.13875	5
GTCTTAC	460	0.0	17.060335	1
GGACGTC	160	1.8189894E-12	16.996593	6
TCTTCGC	95	7.70664E-7	16.841028	29
TACGTGG	95	7.7129334E-7	16.839716	7
GTCCTAT	980	0.0	16.83296	1
TAGAAAT	4705	0.0	16.761786	4
ATGTCCA	4680	0.0	16.752045	9
AATGTCC	4815	0.0	16.612713	8
>>END_MODULE