FastQCFastQC Report
Thu 2 Feb 2017
SRR4062750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567042
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA162220.6319335640009006No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG154970.6036909407793094No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA149130.580941020832538No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC125110.48737028844872815No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA96340.3752957684369792No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA85690.3338083288080211No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT64900.25282017201120977No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA60830.23696534766474409No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA58960.2296806986406923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58570.22816144028808255No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA51820.20186658418522174No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA50850.1980879159748847No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG46110.17962308368932023No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT42260.16462527687509593No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT41650.1622490009902448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40480.15769122593241558No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG40090.15617196757980586No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC37430.14580984650815998No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA35770.13934325967397493No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA34630.13490235064326955No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT34310.1336557796872821No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT33900.13205861064992314No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG33480.13042248627018957No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT33210.12937069202607515No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA31740.1236442566970077No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT30560.11904752629680387No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC29250.11394437644573015No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA29180.11367168904910788No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT29030.11308735891348874No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG28580.11133436850663136No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA28290.11020466357776772No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA28080.10938660138790095No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA27890.10864644988278338No Hit
TTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT26880.1047119603029479No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT25910.10093329209261087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCG252.8937455E-432.0043266
GTGCGCA502.7972648E-522.4021559
TAGGACC39050.020.242274
TGTCCGC951.7262209E-920.21128810
GTCCGCT951.7262209E-920.21089611
CTGACGG400.00448448620.0003661
CGGACCT507.193645E-419.202225
CACGGTT1159.440555E-1018.08623128
TCCGCTG1006.825394E-817.59963612
ACCACGC550.001364031717.4569038
AGCACGT550.001364209717.4565645
CGTATTA550.001364387817.4562249
TAGCGGC2200.017.45384430
CCGTTAA652.1023207E-417.23007616
GTATCAA52450.017.144261
TCGCTAT753.2373195E-517.0659832
GTGTGCG957.693743E-716.8437259
AGGACCT80400.016.8178655
GACGTGT1051.18890966E-716.764177
CTAGCGG2300.016.69498329
Produced by FastQC (version 0.11.5)