##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567042
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288303035166546	32.0	32.0	32.0	32.0	32.0
2	31.377924864493842	32.0	32.0	32.0	32.0	32.0
3	31.429276965472322	32.0	32.0	32.0	32.0	32.0
4	31.50806765140578	32.0	32.0	32.0	32.0	32.0
5	31.471622591293794	32.0	32.0	32.0	32.0	32.0
6	34.99022415683109	36.0	36.0	36.0	36.0	36.0
7	35.01145131244444	36.0	36.0	36.0	36.0	36.0
8	34.936882216964115	36.0	36.0	36.0	36.0	36.0
9	35.062327379139106	36.0	36.0	36.0	36.0	36.0
10	34.935748616501016	36.0	36.0	36.0	36.0	36.0
11	35.06791240657535	36.0	36.0	36.0	36.0	36.0
12	35.00547945845841	36.0	36.0	36.0	36.0	36.0
13	35.042394709552866	36.0	36.0	36.0	36.0	36.0
14	34.999451119225945	36.0	36.0	36.0	36.0	36.0
15	34.96604145939178	36.0	36.0	36.0	36.0	36.0
16	35.003451053780964	36.0	36.0	36.0	36.0	36.0
17	35.010771931273425	36.0	36.0	36.0	36.0	36.0
18	35.01654043837226	36.0	36.0	36.0	36.0	36.0
19	34.96377075248477	36.0	36.0	36.0	36.0	36.0
20	34.950986388224266	36.0	36.0	36.0	36.0	36.0
21	34.917598543381835	36.0	36.0	36.0	36.0	36.0
22	34.89689183114262	36.0	36.0	36.0	36.0	36.0
23	34.875095537977174	36.0	36.0	36.0	32.0	36.0
24	34.86009227741501	36.0	36.0	36.0	32.0	36.0
25	34.827425885513364	36.0	36.0	36.0	32.0	36.0
26	34.76835322522966	36.0	36.0	36.0	32.0	36.0
27	34.69438482112876	36.0	36.0	36.0	32.0	36.0
28	34.63488092520496	36.0	36.0	36.0	32.0	36.0
29	34.60526006197016	36.0	36.0	36.0	32.0	36.0
30	34.537770710413	36.0	36.0	36.0	32.0	36.0
31	34.537363627085185	36.0	36.0	36.0	32.0	36.0
32	34.50708247079713	36.0	36.0	36.0	32.0	36.0
33	34.54816010022431	36.0	36.0	36.0	32.0	36.0
34	34.500413705736015	36.0	36.0	36.0	32.0	36.0
35	34.483645378610866	36.0	36.0	36.0	32.0	36.0
36	34.41345174718606	36.0	36.0	36.0	32.0	36.0
37	34.313381315927046	36.0	36.0	36.0	32.0	36.0
38	33.96481163923302	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	11.0
21	34.0
22	116.0
23	411.0
24	1161.0
25	3058.0
26	6866.0
27	14306.0
28	26111.0
29	43596.0
30	68467.0
31	101117.0
32	144717.0
33	229988.0
34	578159.0
35	1348922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.917541220039936	22.979320049524045	14.382737644896398	25.720401085539617
2	10.594918197676547	22.17174475524748	48.18733000862471	19.04600703845126
3	17.16247727929656	28.68975264136699	31.66675886097695	22.481011218359498
4	10.229307263318473	19.680596610254007	40.979557746755404	29.110538379672118
5	9.450959295157341	41.747365936110874	36.422441246555856	12.379233522175921
6	27.797969645973573	40.1852327973074	20.0003116429818	12.016485913737222
7	25.331394388538982	33.05552871954849	24.407370067844887	17.20570682406764
8	21.65832804499934	44.51557886707719	20.41269652052945	13.413396567394024
9	25.9636072947471	15.873161188230513	22.58768504014308	35.5755464768793
10	15.99372657545581	29.913841697315057	34.72631094666615	19.36612078056298
11	31.472641056647056	20.287528360925503	31.673115400917524	16.566715181509924
12	26.74157285862706	23.73074284592368	34.49963440283004	15.028049892619222
13	29.53055586287064	24.193583572649548	27.223965694287106	19.051894870192704
14	18.058372243227808	27.61372817429555	27.579914937114392	26.747984645362248
15	18.249798795656634	42.33604280724663	22.22164654882935	17.192511848267383
16	16.817488767227026	28.983242190817293	35.53903675904017	18.660232282915512
17	17.522268821468444	33.98199172432707	31.126954681692006	17.368784772512488
18	18.276288428471368	26.978444450850436	39.31427689924824	15.430990221429958
19	24.4737717575326	25.543134861057982	28.79539952988693	21.18769385152249
20	24.349815858096598	29.78451462812062	30.055487989678394	15.810181524104397
21	20.573820654847864	27.217155641188157	27.647963275976718	24.561060427987265
22	23.225573724767155	33.70725282533469	27.777234566958608	15.289938882939541
23	16.563726973939215	33.77251671869163	31.73008413980788	17.933672167561276
24	23.385008893504665	28.10316309589013	32.077737723029074	16.43409028757613
25	19.6784623535278	31.136951508352034	32.06876402393418	17.11582211418599
26	15.553972237306596	33.45769177130721	33.20124875245516	17.787087238931036
27	17.922407069938192	32.5022457789873	30.80803634383285	18.767310807241653
28	16.438687017976335	33.305765936046235	29.083279510035286	21.172267535942147
29	20.161064758582057	29.480234448832547	27.953886223910633	22.404814568674762
30	16.757185897231132	30.12373774951871	31.42340483716277	21.695671516087387
31	22.09083450913542	27.451206485908685	30.460233996950574	19.997725008005325
32	18.389687430123853	32.08397057780901	30.701951896385022	18.824390095682112
33	15.945473428171415	33.13319376932672	34.21907393801894	16.702258864482932
34	18.945112701701024	31.797531945328515	32.9859425751507	16.271412777819762
35	17.50902400506108	33.29466366346947	31.710778397860263	17.48553393360919
36	16.150222707692354	31.534544428957535	33.64109352320687	18.67413934014325
37	17.40851922173459	31.798583739572628	28.213952089603524	22.578944949089262
38	19.10150921022595	34.64923386408771	28.54885669787514	17.700400227811198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	313.0
1	251.5
2	190.0
3	190.0
4	580.5
5	971.0
6	971.0
7	1196.0
8	1421.0
9	1507.5
10	1594.0
11	1594.0
12	2192.0
13	2790.0
14	3905.5
15	5021.0
16	5021.0
17	8140.0
18	11259.0
19	11259.0
20	15176.0
21	19093.0
22	26037.5
23	32982.0
24	32982.0
25	52021.5
26	71061.0
27	71061.0
28	102004.0
29	132947.0
30	191822.0
31	250697.0
32	250697.0
33	315674.0
34	380651.0
35	380651.0
36	390613.5
37	400576.0
38	393461.5
39	386347.0
40	386347.0
41	326763.5
42	267180.0
43	215883.0
44	164586.0
45	164586.0
46	135448.5
47	106311.0
48	106311.0
49	94011.0
50	81711.0
51	71284.0
52	60857.0
53	60857.0
54	51627.5
55	42398.0
56	42398.0
57	40067.0
58	37736.0
59	31774.5
60	25813.0
61	25813.0
62	26968.0
63	28123.0
64	22737.0
65	17351.0
66	17351.0
67	15110.0
68	12869.0
69	12869.0
70	10734.5
71	8600.0
72	6783.0
73	4966.0
74	4966.0
75	3653.0
76	2340.0
77	2340.0
78	2864.5
79	3389.0
80	2618.5
81	1848.0
82	1848.0
83	1931.0
84	2014.0
85	2014.0
86	1276.0
87	538.0
88	458.5
89	379.0
90	379.0
91	225.5
92	72.0
93	48.5
94	25.0
95	25.0
96	20.0
97	15.0
98	15.0
99	11.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008102711213918588
2	0.0
3	0.0
4	2.3373205424765157E-4
5	3.89553423746086E-5
6	7.79106847492172E-5
7	3.8955342374608597E-4
8	0.0027658293085972103
9	0.0038565788950862515
10	8.959728746159977E-4
11	0.005064194508699118
12	3.5059808137147735E-4
13	2.3373205424765157E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1686602712382578E-4
22	3.89553423746086E-5
23	1.9477671187304298E-4
24	0.0
25	7.79106847492172E-5
26	0.0
27	1.558213694984344E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.558213694984344E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2567042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.49178509665259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7444364139632	37.18700334190997
2	10.988143288393912	9.997405061736828
3	2.843017709220618	3.880018519615257
4	1.1637391011973859	2.1176227640097247
5	0.670744702952101	1.5256686940707527
6	0.39448510935909276	1.0767499091276005
7	0.29911595644303823	0.9525123166640476
8	0.2250338092014136	0.8189751750137463
9	0.1837199964922388	0.7521975538544425
>10	1.2032405932138883	10.607265436546328
>50	0.14276436339591983	4.542226689984576
>100	0.11818652512757537	10.79212863445255
>500	0.014521841344618201	4.5219941494256695
>1k	0.007819453031717475	6.852513155957491
>5k	6.874244423487891E-4	2.063806529275138
>10k+	3.4371222117439453E-4	2.3119120683559267
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	16222	0.6319335640009006	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	15497	0.6036909407793094	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	14913	0.580941020832538	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	12511	0.48737028844872815	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	9634	0.3752957684369792	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	8569	0.3338083288080211	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	6490	0.25282017201120977	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6083	0.23696534766474409	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5896	0.2296806986406923	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5857	0.22816144028808255	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	5182	0.20186658418522174	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	5085	0.1980879159748847	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	4611	0.17962308368932023	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	4226	0.16462527687509593	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	4165	0.1622490009902448	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4048	0.15769122593241558	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	4009	0.15617196757980586	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	3743	0.14580984650815998	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	3577	0.13934325967397493	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	3463	0.13490235064326955	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3431	0.1336557796872821	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	3390	0.13205861064992314	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3348	0.13042248627018957	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	3321	0.12937069202607515	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3174	0.1236442566970077	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	3056	0.11904752629680387	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	2925	0.11394437644573015	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	2918	0.11367168904910788	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2903	0.11308735891348874	No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2858	0.11133436850663136	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	2829	0.11020466357776772	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	2808	0.10938660138790095	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	2789	0.10864644988278338	No Hit
TTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT	2688	0.1047119603029479	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2591	0.10093329209261087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.89553423746086E-5	0.0
8	0.0	0.0	0.0	3.89553423746086E-5	0.0
9	0.0	0.0	0.0	3.89553423746086E-5	0.0
10	0.0	0.0	0.0	3.89553423746086E-5	0.0
11	0.0	0.0	0.0	3.89553423746086E-5	0.0
12	0.0	0.0	0.0	3.89553423746086E-5	0.0
13	0.0	0.0	0.0	7.79106847492172E-5	0.0
14	0.0	0.0	0.0	7.79106847492172E-5	0.0
15	0.0	0.0	0.0	1.1686602712382578E-4	0.0
16	0.0	0.0	0.0	1.558213694984344E-4	0.0
17	0.0	0.0	0.0	1.558213694984344E-4	0.0
18	0.0	0.0	0.0	1.9477671187304298E-4	0.0
19	0.0	0.0	0.0	2.3373205424765157E-4	0.0
20	0.0	0.0	0.0	3.116427389968688E-4	0.0
21	0.0	0.0	0.0	3.8955342374608597E-4	0.0
22	0.0	0.0	0.0	5.84330135619129E-4	0.0
23	0.0	0.0	0.0	0.0010907495864890407	0.0
24	3.89553423746086E-5	0.0	0.0	0.002103588488228864	0.0
25	3.89553423746086E-5	0.0	0.0	0.0023762758848511244	0.0
26	7.79106847492172E-5	0.0	0.0	0.0029995613628448618	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTCG	25	2.8937455E-4	32.004326	6
GTGCGCA	50	2.7972648E-5	22.402155	9
TAGGACC	3905	0.0	20.24227	4
TGTCCGC	95	1.7262209E-9	20.211288	10
GTCCGCT	95	1.7262209E-9	20.210896	11
CTGACGG	40	0.004484486	20.000366	1
CGGACCT	50	7.193645E-4	19.20222	5
CACGGTT	115	9.440555E-10	18.086231	28
TCCGCTG	100	6.825394E-8	17.599636	12
ACCACGC	55	0.0013640317	17.456903	8
AGCACGT	55	0.0013642097	17.456564	5
CGTATTA	55	0.0013643878	17.456224	9
TAGCGGC	220	0.0	17.453844	30
CCGTTAA	65	2.1023207E-4	17.230076	16
GTATCAA	5245	0.0	17.14426	1
TCGCTAT	75	3.2373195E-5	17.06598	32
GTGTGCG	95	7.693743E-7	16.843725	9
AGGACCT	8040	0.0	16.817865	5
GACGTGT	105	1.18890966E-7	16.76417	7
CTAGCGG	230	0.0	16.694983	29
>>END_MODULE