FastQCFastQC Report
Thu 2 Feb 2017
SRR4062749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5246024
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG67340.12836388091247772No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG66140.12607643426717072No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC65610.12506614533216012No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA65600.12504708327678257No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA61810.11782256428868795No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC61490.1172125785166061No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG60210.11477263542827865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59830.11404827732393141No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC57270.10916839114727649No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT55940.10663313778206122No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG55250.1053178559610097No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC53740.1024394855989984No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA52920.10087639705803862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69500.017.6760641
TCTAGCG15100.015.25738228
GTCCTAT18650.014.6856181
ACCGTCG12000.014.3988798
GTCTTAG5600.014.3006891
TATAACG901.5312544E-414.227222
CTAGCGG15800.014.17665429
CCTATTC19150.013.9516523
TAGCGGC16150.013.8694230
CGTCGTA12700.013.73226310
CAAGACG16800.013.711524
ATCGTTT12550.013.64089429
CGCTTCG15550.013.58145532
CTATTCC19400.013.52304654
CATCGTT12700.013.47952328
CCGTCGT12750.013.4270479
TCTAGAT11850.013.2367162
TACCGTC13200.013.2110957
ATACCGT14050.013.2075536
TAAACGC16000.013.19922528
Produced by FastQC (version 0.11.5)