##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062749_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5246024
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23991312277641	32.0	32.0	32.0	32.0	32.0
2	30.797738820867004	32.0	32.0	32.0	32.0	32.0
3	30.84428378520571	32.0	32.0	32.0	32.0	32.0
4	30.929863645305474	32.0	32.0	32.0	32.0	32.0
5	30.803329912329794	32.0	32.0	32.0	32.0	32.0
6	34.46672508551238	36.0	36.0	36.0	32.0	36.0
7	34.405927994229536	36.0	36.0	36.0	32.0	36.0
8	34.39256091851657	36.0	36.0	36.0	32.0	36.0
9	34.55447077634414	36.0	36.0	36.0	32.0	36.0
10	34.23007462413439	36.0	36.0	36.0	32.0	36.0
11	34.524409533772626	36.0	36.0	36.0	32.0	36.0
12	34.332867138998985	36.0	36.0	36.0	32.0	36.0
13	34.421720525868736	36.0	36.0	36.0	32.0	36.0
14	34.31465086701853	36.0	36.0	36.0	32.0	36.0
15	34.24616585818136	36.0	36.0	36.0	32.0	36.0
16	34.270645159076665	36.0	36.0	36.0	32.0	36.0
17	34.191196037227435	36.0	36.0	36.0	32.0	36.0
18	34.2157443427632	36.0	36.0	36.0	32.0	36.0
19	34.2060884586117	36.0	36.0	36.0	32.0	36.0
20	34.1942213760364	36.0	36.0	36.0	32.0	36.0
21	34.1854261436852	36.0	36.0	36.0	32.0	36.0
22	34.15834658781584	36.0	36.0	36.0	32.0	36.0
23	34.09325729352363	36.0	36.0	36.0	32.0	36.0
24	34.07723239542938	36.0	36.0	36.0	32.0	36.0
25	34.05586516569501	36.0	36.0	36.0	32.0	36.0
26	33.9980082058336	36.0	36.0	36.0	32.0	36.0
27	34.001355502757896	36.0	36.0	36.0	32.0	36.0
28	33.988679998414035	36.0	36.0	36.0	32.0	36.0
29	33.95579109054781	36.0	36.0	36.0	32.0	36.0
30	33.928528348326275	36.0	36.0	36.0	32.0	36.0
31	33.955901841089556	36.0	36.0	36.0	32.0	36.0
32	33.90979225409567	36.0	36.0	36.0	32.0	36.0
33	33.8712485875017	36.0	36.0	36.0	32.0	36.0
34	33.88979844545126	36.0	36.0	36.0	32.0	36.0
35	33.84967167515818	36.0	36.0	36.0	32.0	36.0
36	33.80098527951836	36.0	36.0	36.0	32.0	36.0
37	33.8112665897068	36.0	36.0	36.0	32.0	36.0
38	33.20683073504811	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	5.0
8	12.0
9	14.0
10	43.0
11	43.0
12	24.0
13	25.0
14	397.0
15	891.0
16	1337.0
17	1695.0
18	2166.0
19	2882.0
20	4167.0
21	6234.0
22	9954.0
23	15509.0
24	24270.0
25	36731.0
26	54134.0
27	77713.0
28	108297.0
29	145931.0
30	196348.0
31	262715.0
32	355235.0
33	528208.0
34	1140484.0
35	2270557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13824793322813	17.287060851535202	11.58361789856118	25.991073316675482
2	16.991053325111853	19.383286125949972	37.17322531739821	26.452435231539962
3	18.668826031338114	23.87909718475532	28.21019298924575	29.241883794660815
4	12.761072541013293	15.36045567361691	35.839233223913716	36.03923856145608
5	15.13148840243278	35.992317214555655	33.32352906416185	15.552665318849701
6	34.53498286602906	35.13164573997272	16.859514781802783	13.473856612195437
7	30.802266783809372	29.988831539625348	21.079129847505435	18.129771829059845
8	28.42450869308625	32.27871554904224	19.413332957174294	19.883442800697214
9	27.488144341098337	14.68180246426909	18.49398915520305	39.33606403942952
10	16.0141356982041	25.93419111556995	31.034277676610923	27.01739550961502
11	37.60027541366855	21.084662443834997	22.347413526722733	18.967648615773722
12	25.52979409085866	23.16901980325229	28.05206574422035	23.249120361668698
13	29.09748953871196	19.117738176966732	25.465241108643927	26.319531175677387
14	24.045574508289288	19.701942441270376	24.525179751881783	31.727303298558553
15	25.47938654994978	26.677671794003537	21.938831931190116	25.90410972485656
16	26.57212048699461	25.3795887849327	22.95899001788016	25.089300710192532
17	24.624999356528924	25.560463306800717	24.71302619966364	25.101511137006717
18	25.628147313326764	24.354429038092597	25.477301217901598	24.54012243067904
19	25.961787937024045	24.760976822381945	24.88805424562697	24.38918099496704
20	26.00002783216594	24.080980927531712	24.30194163671055	25.617049603591795
21	27.30426480980507	23.953747233208475	23.9480663866022	24.79392157038426
22	26.162677203901442	23.84952337669369	24.417784481319003	25.570014938085865
23	24.59999306076369	23.588885326069757	25.206242109954868	26.604879503211688
24	25.21719402666382	24.83477620960439	24.410149257145907	25.537880506585886
25	25.289712940918086	24.128249261418837	24.65299649660108	25.929041301062
26	25.23845757429415	24.943265695759994	25.075256484049856	24.743020245896
27	25.89234548545646	24.411473479084588	24.05412350792804	25.642057527530916
28	24.98186617244798	24.209465200303256	25.146571843984837	25.66209678326392
29	25.00827851971489	24.5471406650611	25.079520495937185	25.365060319286826
30	24.966711953025207	24.639712292148673	25.321581119801323	25.0719946350248
31	25.680946907091702	24.438464955172893	24.054284358075456	25.82630377965995
32	25.135473534954706	24.43182706359564	24.014111437348184	26.41858796410147
33	24.764811696848245	24.13197462856376	24.817751464851195	26.2854622097368
34	25.74983475963365	24.12293921385049	24.902690725130206	25.224535301385654
35	26.40777013694399	23.99637002141001	24.800799205370232	24.795060636275764
36	25.18898895769149	24.773682243845897	24.237924381539212	25.799404416923405
37	26.284723287949095	24.385783871797333	24.350191506750445	24.97930133350313
38	25.182363824050007	24.20188363982275	24.88120258612062	25.734549950006624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	210.0
2	317.0
3	317.0
4	724.0
5	1131.0
6	1131.0
7	1315.0
8	1499.0
9	1507.5
10	1516.0
11	1516.0
12	2257.0
13	2998.0
14	4283.5
15	5569.0
16	5569.0
17	8514.0
18	11459.0
19	11459.0
20	15601.0
21	19743.0
22	24318.0
23	28893.0
24	28893.0
25	36074.5
26	43256.0
27	43256.0
28	56520.0
29	69784.0
30	87019.0
31	104254.0
32	104254.0
33	137847.0
34	171440.0
35	171440.0
36	193148.5
37	214857.0
38	246657.0
39	278457.0
40	278457.0
41	301036.5
42	323616.0
43	369906.5
44	416197.0
45	416197.0
46	424834.5
47	433472.0
48	433472.0
49	467113.0
50	500754.0
51	506134.0
52	511514.0
53	511514.0
54	487390.5
55	463267.0
56	463267.0
57	448650.0
58	434033.0
59	391273.0
60	348513.0
61	348513.0
62	328692.5
63	308872.0
64	256189.5
65	203507.0
66	203507.0
67	169470.5
68	135434.0
69	135434.0
70	108785.0
71	82136.0
72	64011.5
73	45887.0
74	45887.0
75	34344.0
76	22801.0
77	22801.0
78	22324.5
79	21848.0
80	17259.0
81	12670.0
82	12670.0
83	12641.5
84	12613.0
85	12613.0
86	8437.5
87	4262.0
88	3724.5
89	3187.0
90	3187.0
91	2346.0
92	1505.0
93	1306.0
94	1107.0
95	1107.0
96	1029.0
97	951.0
98	951.0
99	1776.5
100	2602.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12298838129600627
2	0.054269671659908536
3	0.012466584216923138
4	0.003126177081919564
5	1.9062055377558318E-4
6	2.478067199082581E-4
7	1.9062055377558318E-5
8	3.049928860409331E-4
9	1.1437233226534991E-4
10	2.859308306633748E-4
11	0.001849019371623157
12	0.0022493225345518817
13	0.013972486591750247
14	0.008768545473676826
15	0.02279821823155975
16	0.010388820180769283
17	0.019691103205017744
18	0.005337375505716329
19	0.007682008317156003
20	0.005623306336379704
21	0.006233292108461569
22	0.007091084600451694
23	0.009340407135003576
24	0.013610307539576639
25	0.01585963007412852
26	0.018318635217833543
27	0.008882917805942176
28	0.00577580277940017
29	0.009931330851707885
30	0.002802122140501073
31	0.005985485388553312
32	0.006671719382145412
33	0.008387304366125661
34	0.012104405164749532
35	0.01551651307733247
36	0.014182169200903387
37	0.00985508263019765
38	0.006271416219216687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	5246024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.07187457107518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79607063879273	32.216069729633595
2	13.380079667237341	11.526102263564802
3	4.662353834650861	6.024489587161598
4	2.2192499294344348	3.823490184098697
5	1.2522523625757347	2.69684283460973
6	0.7659450266911523	1.9794412870788085
7	0.5181915708792456	1.5623637641289498
8	0.358801416054402	1.2363399670575492
9	0.2990424534769465	1.159228714282706
>10	1.5435932619250798	12.035389863589101
>50	0.10449023448781003	3.0981075218591627
>100	0.07344804462523327	6.60436723172704
>500	0.013752609409646316	4.2572566113765555
>1k	0.0120168406821106	9.988621287205104
>5k	7.121090774583219E-4	1.7918891526265857
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	6734	0.12836388091247772	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	6614	0.12607643426717072	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6561	0.12506614533216012	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	6560	0.12504708327678257	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	6181	0.11782256428868795	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6149	0.1172125785166061	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	6021	0.11477263542827865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5983	0.11404827732393141	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5727	0.10916839114727649	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5594	0.10663313778206122	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	5525	0.1053178559610097	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	5374	0.1024394855989984	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	5292	0.10087639705803862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9062055377558318E-5
2	0.0	0.0	0.0	1.9062055377558318E-5	1.9062055377558318E-5
3	0.0	0.0	0.0	3.8124110755116636E-5	1.9062055377558318E-5
4	0.0	0.0	0.0	5.718616613267496E-5	1.9062055377558318E-5
5	0.0	1.9062055377558318E-5	0.0	5.718616613267496E-5	1.9062055377558318E-5
6	0.0	1.9062055377558318E-5	0.0	7.624822151023327E-5	3.8124110755116636E-5
7	0.0	1.9062055377558318E-5	0.0	7.624822151023327E-5	3.8124110755116636E-5
8	0.0	1.9062055377558318E-5	0.0	7.624822151023327E-5	5.718616613267496E-5
9	0.0	1.9062055377558318E-5	0.0	9.531027688779159E-5	5.718616613267496E-5
10	0.0	1.9062055377558318E-5	0.0	9.531027688779159E-5	5.718616613267496E-5
11	0.0	1.9062055377558318E-5	0.0	9.531027688779159E-5	5.718616613267496E-5
12	0.0	1.9062055377558318E-5	0.0	9.531027688779159E-5	1.5249644302046654E-4
13	0.0	1.9062055377558318E-5	0.0	9.531027688779159E-5	1.7155849839802488E-4
14	0.0	1.9062055377558318E-5	0.0	1.1437233226534991E-4	1.7155849839802488E-4
15	1.9062055377558318E-5	1.9062055377558318E-5	0.0	1.1437233226534991E-4	2.4780671990825816E-4
16	1.9062055377558318E-5	1.9062055377558318E-5	0.0	1.1437233226534991E-4	2.6686877528581647E-4
17	1.9062055377558318E-5	1.9062055377558318E-5	0.0	1.5249644302046654E-4	2.859308306633748E-4
18	1.9062055377558318E-5	1.9062055377558318E-5	0.0	2.2874466453069983E-4	3.049928860409331E-4
19	1.9062055377558318E-5	1.9062055377558318E-5	0.0	2.6686877528581647E-4	3.049928860409331E-4
20	1.9062055377558318E-5	1.9062055377558318E-5	0.0	4.5748932906139965E-4	3.240549414184914E-4
21	1.9062055377558318E-5	1.9062055377558318E-5	0.0	5.337375505716329E-4	4.19365218306283E-4
22	1.9062055377558318E-5	1.9062055377558318E-5	0.0	7.243581043472161E-4	4.19365218306283E-4
23	1.9062055377558318E-5	1.9062055377558318E-5	0.0	9.721648242554743E-4	5.146754951940746E-4
24	1.9062055377558318E-5	1.9062055377558318E-5	0.0	0.0015440264855822237	5.146754951940746E-4
25	1.9062055377558318E-5	1.9062055377558318E-5	0.0	0.0019443296485109486	5.337375505716329E-4
26	1.9062055377558318E-5	1.9062055377558318E-5	0.0	0.0024780671990825816	5.337375505716329E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6950	0.0	17.676064	1
TCTAGCG	1510	0.0	15.257382	28
GTCCTAT	1865	0.0	14.685618	1
ACCGTCG	1200	0.0	14.398879	8
GTCTTAG	560	0.0	14.300689	1
TATAACG	90	1.5312544E-4	14.22722	2
CTAGCGG	1580	0.0	14.176654	29
CCTATTC	1915	0.0	13.951652	3
TAGCGGC	1615	0.0	13.86942	30
CGTCGTA	1270	0.0	13.732263	10
CAAGACG	1680	0.0	13.71152	4
ATCGTTT	1255	0.0	13.640894	29
CGCTTCG	1555	0.0	13.581455	32
CTATTCC	1940	0.0	13.5230465	4
CATCGTT	1270	0.0	13.479523	28
CCGTCGT	1275	0.0	13.427047	9
TCTAGAT	1185	0.0	13.236716	2
TACCGTC	1320	0.0	13.211095	7
ATACCGT	1405	0.0	13.207553	6
TAAACGC	1600	0.0	13.199225	28
>>END_MODULE