FastQCFastQC Report
Thu 2 Feb 2017
SRR4062749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5246024
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC69740.13293877420309172No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC69520.13251940898478543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA68780.1311088168868461No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA67460.1285926255770084No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG66430.12662923387311992No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG63080.12024344532163787No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA62420.11898534966671903No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC60240.11482982159441131No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT59220.11288549194590036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT57980.11052179707908313No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG57920.11040742474681776No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG57460.10953057019945009No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT55760.10629002078526519No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG55300.10541316623789751No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC52930.10089545911341619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68700.017.5839841
ACCGTCG12550.016.0652338
TCTAGCG13650.015.35483128
TACCGTC14000.014.7443637
CGTATAC1205.4246993E-714.6671873
CGTCGTA13450.014.63262910
CTAGCGG14350.014.60581529
CCGTCGT14050.014.5774479
ATACCGT14750.014.5370796
CGCGTAA7850.013.45283510
CCTATAC3100.013.419833
AACCGCG12250.013.3238117
CGAAAAA12250.013.32203324
GCGTAAC7950.013.2834911
TCGCGTA8100.013.2354159
TAGCGGC15600.013.23035530
GTATTAG13450.013.2046791
TTCGTTC18050.013.11870329
CTTTCGT18550.013.11010327
TTTCGTT18250.012.974936528
Produced by FastQC (version 0.11.5)