##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5246024
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173790665082738	32.0	32.0	32.0	32.0	32.0
2	31.190346822660363	32.0	32.0	32.0	32.0	32.0
3	31.311960448522537	32.0	32.0	32.0	32.0	32.0
4	31.42836994264609	32.0	32.0	32.0	32.0	32.0
5	31.33498550521309	32.0	32.0	32.0	32.0	32.0
6	34.85284417303466	36.0	36.0	36.0	32.0	36.0
7	34.8805358115022	36.0	36.0	36.0	32.0	36.0
8	34.81239430090293	36.0	36.0	36.0	32.0	36.0
9	34.94332545943366	36.0	36.0	36.0	32.0	36.0
10	34.76304340201265	36.0	36.0	36.0	32.0	36.0
11	34.97431216479376	36.0	36.0	36.0	36.0	36.0
12	34.85410874978841	36.0	36.0	36.0	32.0	36.0
13	34.92129182024329	36.0	36.0	36.0	32.0	36.0
14	34.85906507480713	36.0	36.0	36.0	32.0	36.0
15	34.824751850163096	36.0	36.0	36.0	32.0	36.0
16	34.83920679737645	36.0	36.0	36.0	32.0	36.0
17	34.80125672318693	36.0	36.0	36.0	32.0	36.0
18	34.80509620238108	36.0	36.0	36.0	32.0	36.0
19	34.8036985724808	36.0	36.0	36.0	32.0	36.0
20	34.78674649601298	36.0	36.0	36.0	32.0	36.0
21	34.78290491999274	36.0	36.0	36.0	32.0	36.0
22	34.76773590818494	36.0	36.0	36.0	32.0	36.0
23	34.705721514045685	36.0	36.0	36.0	32.0	36.0
24	34.67962841954211	36.0	36.0	36.0	32.0	36.0
25	34.658925502437654	36.0	36.0	36.0	32.0	36.0
26	34.58789246865817	36.0	36.0	36.0	32.0	36.0
27	34.57508009875669	36.0	36.0	36.0	32.0	36.0
28	34.54152249398783	36.0	36.0	36.0	32.0	36.0
29	34.507869769562625	36.0	36.0	36.0	32.0	36.0
30	34.49188623612854	36.0	36.0	36.0	32.0	36.0
31	34.49069714511409	36.0	36.0	36.0	32.0	36.0
32	34.46522299554863	36.0	36.0	36.0	32.0	36.0
33	34.43340232526576	36.0	36.0	36.0	32.0	36.0
34	34.43369549967747	36.0	36.0	36.0	32.0	36.0
35	34.398128563651255	36.0	36.0	36.0	32.0	36.0
36	34.37791916316052	36.0	36.0	36.0	32.0	36.0
37	34.374708922414385	36.0	36.0	36.0	32.0	36.0
38	33.91733148761805	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	22.0
21	115.0
22	447.0
23	1303.0
24	3614.0
25	8929.0
26	18877.0
27	36162.0
28	63062.0
29	99949.0
30	152159.0
31	220410.0
32	319632.0
33	516731.0
34	1236898.0
35	2567710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44258909266156	18.505850420332752	12.315164348980842	26.736396138024855
2	15.940014761655686	20.477088934400605	36.35566669157442	27.227229612369293
3	17.640693218330682	24.678461249891345	28.533895384390156	29.146950147387813
4	12.24532082656311	16.334808231471833	35.85359697995354	35.56627396201152
5	14.710536860119916	36.56280892455273	32.8255390465385	15.901115168788845
6	34.566065861420526	35.56032531359518	16.495454168545077	13.37815465643922
7	30.65075971114793	30.20222809462511	20.950963275496964	18.19604891873
8	28.33967826787957	32.5648690209929	19.12287213084004	19.972580580287488
9	27.463907550257975	14.48928807125807	18.20286345317065	39.843940925313305
10	15.82058022747717	26.21850991721847	30.946532933712824	27.014376921591538
11	37.83287992065231	21.00047028340723	22.17023115563712	18.996418640303343
12	25.438719391720216	23.260014712142016	27.912658141706636	23.38860775443113
13	29.106027758604487	19.134855365398444	25.33167569352192	26.427441182475146
14	23.95465594515008	19.612090985477764	24.438126855691092	31.995126213681065
15	25.433966752725496	26.85744480010004	21.714464135124047	25.99412431205042
16	26.548630562357534	25.505058880805304	22.747769643360243	25.19854091347692
17	24.668701477538036	25.714808014603058	24.423582507438013	25.19290800042089
18	25.724224669959572	24.41590049912086	25.31236990147205	24.54750492944752
19	25.995058352763923	24.777393317300874	24.742490693904564	24.48505763603064
20	26.049823637863646	24.06622615527493	24.192588520372762	25.691361686488662
21	27.351912889340618	24.027867237182036	23.840887286488016	24.77933258698933
22	26.076401113757985	23.964096230611265	24.327190330656194	25.632312324974553
23	24.535928877089034	23.58751410832256	25.10327358832425	26.773283426264154
24	25.226533466106904	24.838220336010664	24.330902794192326	25.60434340369011
25	25.301857523172817	24.177252155825617	24.59940091703841	25.92148940396316
26	25.30963002612076	24.87802655689962	24.940073606290788	24.87226981068883
27	25.97521128886925	24.397557232467985	23.957890338517775	25.66934114014499
28	25.011036930063607	24.248516590850517	25.09914175001868	25.641304729067194
29	25.089591660274525	24.446380725669574	25.023408204003644	25.44061941005226
30	25.03795255225672	24.525221386711156	25.265248500578725	25.1715775604534
31	25.728742377084053	24.40705570542567	24.0177513484498	25.846450569040474
32	25.2954237342414	24.41378461097395	23.966550667705675	26.32424098707898
33	24.828517749823487	24.126061947105086	24.78656216593748	26.25885813713395
34	25.680534439034208	24.07192570983282	24.995901658093825	25.25163819303915
35	26.45271924032372	23.99157914641641	24.697275498548997	24.858426114710873
36	25.24351775744831	24.733474341710977	24.229645156026734	25.793362744813976
37	26.37622321209358	24.38643056150715	24.246114771872946	24.99123145452632
38	25.287632393249055	24.11213150390801	24.765198143582783	25.835037959260156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	92.0
2	134.0
3	134.0
4	471.5
5	809.0
6	809.0
7	1004.0
8	1199.0
9	1245.5
10	1292.0
11	1292.0
12	2013.0
13	2734.0
14	3999.0
15	5264.0
16	5264.0
17	8254.0
18	11244.0
19	11244.0
20	15116.0
21	18988.0
22	23655.0
23	28322.0
24	28322.0
25	35821.0
26	43320.0
27	43320.0
28	56496.5
29	69673.0
30	87138.0
31	104603.0
32	104603.0
33	139178.5
34	173754.0
35	173754.0
36	194266.5
37	214779.0
38	246110.5
39	277442.0
40	277442.0
41	300180.5
42	322919.0
43	370001.5
44	417084.0
45	417084.0
46	425627.5
47	434171.0
48	434171.0
49	468039.0
50	501907.0
51	505449.0
52	508991.0
53	508991.0
54	487359.0
55	465727.0
56	465727.0
57	451061.5
58	436396.0
59	392853.5
60	349311.0
61	349311.0
62	330307.5
63	311304.0
64	257759.0
65	204214.0
66	204214.0
67	170390.0
68	136566.0
69	136566.0
70	110029.0
71	83492.0
72	64928.0
73	46364.0
74	46364.0
75	34351.0
76	22338.0
77	22338.0
78	22366.5
79	22395.0
80	17323.0
81	12251.0
82	12251.0
83	11716.0
84	11181.0
85	11181.0
86	7071.5
87	2962.0
88	2542.5
89	2123.0
90	2123.0
91	1275.0
92	427.0
93	286.5
94	146.0
95	146.0
96	115.5
97	85.0
98	85.0
99	74.0
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007967939147819377
2	0.0
3	0.0
4	2.2874466453069983E-4
5	3.8124110755116636E-5
6	1.7155849839802485E-4
7	3.240549414184914E-4
8	0.00203963992539874
9	0.0036217905217360803
10	8.959166027452409E-4
11	0.0047845758997671385
12	3.240549414184914E-4
13	2.0968260915314152E-4
14	0.0
15	0.0
16	3.8124110755116636E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3343438764290823E-4
22	1.9062055377558318E-5
23	1.3343438764290823E-4
24	0.0
25	1.1437233226534991E-4
26	9.531027688779159E-5
27	1.3343438764290823E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.531027688779159E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	5246024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.547726519234935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28254706376052	29.308929504550896
2	14.641726117820806	11.873774127898749
3	5.16069762376864	6.277636676911093
4	2.4628219478843256	3.9944732323353245
5	1.358241411864903	2.753680065769882
6	0.8435264607627122	2.0521848145646766
7	0.5777429120124999	1.6398313116296481
8	0.41075331419975925	1.3324090440832976
9	0.31390838456797515	1.1455444196601376
>10	1.7226332874612167	12.633039811235728
>50	0.11519723466732598	3.2149761064093374
>100	0.08136751910427288	6.9237623295432575
>500	0.014299984802254072	4.132549921541254
>1k	0.013731771412610786	10.743572594591846
>5k	8.049659103943687E-4	1.9736360392749186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6974	0.13293877420309172	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6952	0.13251940898478543	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	6878	0.1311088168868461	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	6746	0.1285926255770084	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	6643	0.12662923387311992	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	6308	0.12024344532163787	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	6242	0.11898534966671903	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	6024	0.11482982159441131	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5922	0.11288549194590036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5798	0.11052179707908313	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	5792	0.11040742474681776	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	5746	0.10953057019945009	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	5576	0.10629002078526519	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	5530	0.10541316623789751	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	5293	0.10089545911341619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8124110755116636E-5
2	0.0	0.0	0.0	0.0	3.8124110755116636E-5
3	0.0	0.0	0.0	0.0	3.8124110755116636E-5
4	0.0	0.0	0.0	1.9062055377558318E-5	3.8124110755116636E-5
5	0.0	0.0	0.0	1.9062055377558318E-5	3.8124110755116636E-5
6	0.0	0.0	0.0	1.9062055377558318E-5	9.531027688779159E-5
7	0.0	0.0	0.0	3.8124110755116636E-5	9.531027688779159E-5
8	0.0	0.0	0.0	3.8124110755116636E-5	9.531027688779159E-5
9	0.0	0.0	0.0	1.1437233226534991E-4	9.531027688779159E-5
10	0.0	0.0	0.0	1.3343438764290823E-4	1.3343438764290823E-4
11	0.0	0.0	0.0	1.3343438764290823E-4	1.3343438764290823E-4
12	0.0	0.0	0.0	1.3343438764290823E-4	1.7155849839802488E-4
13	0.0	0.0	0.0	1.3343438764290823E-4	2.2874466453069983E-4
14	0.0	0.0	0.0	1.5249644302046654E-4	2.6686877528581647E-4
15	0.0	0.0	0.0	1.9062055377558318E-4	3.049928860409331E-4
16	0.0	0.0	0.0	2.2874466453069983E-4	3.4311699679604975E-4
17	0.0	0.0	0.0	3.049928860409331E-4	3.4311699679604975E-4
18	0.0	0.0	0.0	3.6217905217360806E-4	3.8124110755116637E-4
19	0.0	0.0	0.0	4.19365218306283E-4	3.8124110755116637E-4
20	0.0	3.8124110755116636E-5	0.0	6.290478274594245E-4	4.3842727368384135E-4
21	0.0	3.8124110755116636E-5	0.0	7.243581043472161E-4	4.3842727368384135E-4
22	0.0	3.8124110755116636E-5	0.0	9.340407135003576E-4	4.3842727368384135E-4
23	0.0	3.8124110755116636E-5	0.0	0.001200909488786174	4.3842727368384135E-4
24	0.0	3.8124110755116636E-5	0.0	0.0018490193716231569	4.5748932906139965E-4
25	0.0	5.718616613267496E-5	0.0	0.0022302604791743235	4.5748932906139965E-4
26	0.0	5.718616613267496E-5	0.0	0.0027639980297459563	5.146754951940746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6870	0.0	17.583984	1
ACCGTCG	1255	0.0	16.065233	8
TCTAGCG	1365	0.0	15.354831	28
TACCGTC	1400	0.0	14.744363	7
CGTATAC	120	5.4246993E-7	14.667187	3
CGTCGTA	1345	0.0	14.632629	10
CTAGCGG	1435	0.0	14.605815	29
CCGTCGT	1405	0.0	14.577447	9
ATACCGT	1475	0.0	14.537079	6
CGCGTAA	785	0.0	13.452835	10
CCTATAC	310	0.0	13.41983	3
AACCGCG	1225	0.0	13.323811	7
CGAAAAA	1225	0.0	13.322033	24
GCGTAAC	795	0.0	13.28349	11
TCGCGTA	810	0.0	13.235415	9
TAGCGGC	1560	0.0	13.230355	30
GTATTAG	1345	0.0	13.204679	1
TTCGTTC	1805	0.0	13.118703	29
CTTTCGT	1855	0.0	13.110103	27
TTTCGTT	1825	0.0	12.9749365	28
>>END_MODULE