FastQCFastQC Report
Thu 2 Feb 2017
SRR4062748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659763
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA92430.5568867362388485No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC73100.44042432564167294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61300.3693298380551922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40340.24304674824056205No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG32750.19731732783535963No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24340.1466474430385543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21730.1309223063774768No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA18830.11344993230961288No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18320.11037720445629888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.11019645575904512No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA18180.10953371053578133No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17870.10766597399749242No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG17590.10597898615645727No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17440.10507524267018846No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16720.10073727393609812No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16670.10043602610734183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA400.004484475619.99986632
CATCGTT3450.018.08683628
CGGCATC3750.017.91988225
CGACGGA450.00885221717.7776624
TAGCGTA2450.017.6314747
TCGTTTA3600.017.3332230
ATCGTTT3600.017.3332229
CGCAAGA4550.017.2342912
GTCCTAC2700.017.1980271
GCATCGT3750.017.0660427
TCTAGCG4150.016.57820528
ATAGCGT2750.016.2873656
CTAGCGG4250.016.18812829
TAGCGGC4400.015.99989430
TATTCCA6200.015.9965215
AAGACGG4700.015.996525
CTATTCC5650.015.8549584
CCTATTC5150.015.8412143
GGCATCG4150.015.80712626
CAAGACG4900.015.6700614
Produced by FastQC (version 0.11.5)