##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062748_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659763
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151375828958713	32.0	32.0	32.0	32.0	32.0
2	30.762425719816623	32.0	32.0	32.0	32.0	32.0
3	30.833066528173	32.0	32.0	32.0	32.0	32.0
4	30.88553124753353	32.0	32.0	32.0	32.0	32.0
5	30.795631062989113	32.0	32.0	32.0	32.0	32.0
6	34.44299637960359	36.0	36.0	36.0	32.0	36.0
7	34.35408549292881	36.0	36.0	36.0	32.0	36.0
8	34.336157632143866	36.0	36.0	36.0	32.0	36.0
9	34.47500757638289	36.0	36.0	36.0	32.0	36.0
10	34.18424497955431	36.0	36.0	36.0	32.0	36.0
11	34.49777407979332	36.0	36.0	36.0	32.0	36.0
12	34.29741716136581	36.0	36.0	36.0	32.0	36.0
13	34.38417292107367	36.0	36.0	36.0	32.0	36.0
14	34.278922954662804	36.0	36.0	36.0	32.0	36.0
15	34.21790279696559	36.0	36.0	36.0	32.0	36.0
16	34.22352167146755	36.0	36.0	36.0	32.0	36.0
17	34.153894863302774	36.0	36.0	36.0	32.0	36.0
18	34.17608779084725	36.0	36.0	36.0	32.0	36.0
19	34.169352491891914	36.0	36.0	36.0	32.0	36.0
20	34.138099837145425	36.0	36.0	36.0	32.0	36.0
21	34.0935717930813	36.0	36.0	36.0	32.0	36.0
22	34.0631644397423	36.0	36.0	36.0	32.0	36.0
23	33.971417003511945	36.0	36.0	36.0	32.0	36.0
24	33.96851598692103	36.0	36.0	36.0	32.0	36.0
25	33.91727192376261	36.0	36.0	36.0	32.0	36.0
26	33.8554462293713	36.0	36.0	36.0	32.0	36.0
27	33.831827797101155	36.0	36.0	36.0	32.0	36.0
28	33.82641678360103	36.0	36.0	36.0	32.0	36.0
29	33.770496149149004	36.0	36.0	36.0	32.0	36.0
30	33.71838388974812	36.0	36.0	36.0	27.0	36.0
31	33.74211438621056	36.0	36.0	36.0	32.0	36.0
32	33.68048691289057	36.0	36.0	36.0	27.0	36.0
33	33.615089624241534	36.0	36.0	36.0	27.0	36.0
34	33.61875520782184	36.0	36.0	36.0	27.0	36.0
35	33.53202113795765	36.0	36.0	36.0	27.0	36.0
36	33.48962833850375	36.0	36.0	36.0	21.0	36.0
37	33.43631651024875	36.0	36.0	36.0	21.0	36.0
38	32.808835960314816	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	8.0
8	1.0
9	8.0
10	18.0
11	12.0
12	15.0
13	10.0
14	237.0
15	448.0
16	652.0
17	822.0
18	1155.0
19	1524.0
20	2078.0
21	2617.0
22	3689.0
23	5534.0
24	8148.0
25	12321.0
26	17949.0
27	25852.0
28	36047.0
29	49948.0
30	67002.0
31	88817.0
32	118036.0
33	171256.0
34	359426.0
35	686132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26582236482936	16.99347469778929	11.758574073370289	24.98212886401106
2	15.604918534808073	20.29763670165438	38.68155874970161	25.41588601383594
3	19.121189807913172	25.71636371524535	27.938998362218463	27.223448114623018
4	11.826016646161225	16.134629775347918	36.61613209923107	35.42322147925978
5	13.608878863355109	37.94074090140634	33.75652653814222	14.693853697096332
6	32.908351699464625	35.74394580414735	18.324056880581384	13.023645615806641
7	29.632260528919208	31.01896536973373	21.341734537843376	18.00703956350368
8	28.53772306016434	32.56175312727066	20.01724351922032	18.883280293344676
9	28.043898045193416	14.68367319273025	18.3940400842293	38.87838867784703
10	15.223057420626695	27.626054976888113	32.34501298989246	24.805874612592724
11	37.055784040941795	21.846851460666304	21.708816295741094	19.388548202650803
12	24.42414499917455	24.441738476573562	29.442142162527578	21.691974361724306
13	30.09281549162443	20.309256421550934	25.13604763761284	24.461880449211794
14	23.94161498767176	19.967715189888672	25.8570719280021	30.23359789443747
15	25.253499822221688	27.161332312865728	24.03803853269614	23.547129332216443
16	25.32020226853124	26.15318611500769	24.946010306246897	23.580601310214174
17	23.45627082445717	26.14940156332955	26.304456953598482	24.089870658614796
18	24.70462127835464	24.656478568661207	27.703207123915963	22.935693029068194
19	24.479252654500932	26.74649952941432	26.240484347753785	22.53376346833097
20	25.015816549714582	24.10984458206349	27.36010035826745	23.514238509954485
21	26.336424744464505	25.122616066500687	24.663001641307936	23.877957547726872
22	24.717724077884597	25.558631867850462	25.693902574963772	24.029741479301173
23	23.24849783442315	25.26512348728974	25.93227058985155	25.554108088435555
24	23.377193088171847	26.625337745822314	25.926887821385787	24.07058134462005
25	24.627623867264283	25.08029633414845	25.495366615285047	24.79671318330222
26	23.343039337034952	25.87977732434885	26.645270070758215	24.131913267857982
27	25.065919180915014	25.841644532925844	25.483427411774347	23.60900887438479
28	23.676883411732877	25.077952666237262	26.551325843048673	24.69383807898119
29	23.105985285345	26.217453918786195	27.072976734937367	23.603584060931446
30	24.076416081672445	25.738821945854166	27.144193870831106	23.040568101642283
31	23.669050486816285	25.85799148139289	25.805751897526065	24.66720613426477
32	23.192999940348617	26.403630178297384	25.764215557201158	24.639154324152845
33	22.888311782215208	25.762644460780177	26.300779696557044	25.048264060447572
34	23.49465397890515	26.41947947397852	26.860020053604828	23.2258464935115
35	24.82309419891184	25.64418538366097	26.477689953413652	23.055030464013537
36	23.54560705358886	26.761348434852405	26.025113225238076	23.667931286320655
37	24.710108773984867	26.50513259917522	25.820504848191238	22.964253778648676
38	23.21359172407311	26.666714869397712	25.791112259942867	24.328581146586313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	244.5
2	344.0
3	344.0
4	794.0
5	1244.0
6	1244.0
7	1612.5
8	1981.0
9	2086.5
10	2192.0
11	2192.0
12	2765.5
13	3339.0
14	4219.5
15	5100.0
16	5100.0
17	7425.0
18	9750.0
19	9750.0
20	11746.5
21	13743.0
22	14272.5
23	14802.0
24	14802.0
25	17030.5
26	19259.0
27	19259.0
28	25335.5
29	31412.0
30	38421.5
31	45431.0
32	45431.0
33	54792.0
34	64153.0
35	64153.0
36	71591.0
37	79029.0
38	90193.5
39	101358.0
40	101358.0
41	109891.5
42	118425.0
43	131356.0
44	144287.0
45	144287.0
46	159807.5
47	175328.0
48	175328.0
49	168278.0
50	161228.0
51	152029.5
52	142831.0
53	142831.0
54	131759.5
55	120688.0
56	120688.0
57	114878.0
58	109068.0
59	96880.0
60	84692.0
61	84692.0
62	79896.0
63	75100.0
64	62232.5
65	49365.0
66	49365.0
67	41148.5
68	32932.0
69	32932.0
70	26631.0
71	20330.0
72	15772.0
73	11214.0
74	11214.0
75	8557.0
76	5900.0
77	5900.0
78	5474.0
79	5048.0
80	4085.5
81	3123.0
82	3123.0
83	3097.0
84	3071.0
85	3071.0
86	2132.5
87	1194.0
88	1009.5
89	825.0
90	825.0
91	609.0
92	393.0
93	345.5
94	298.0
95	298.0
96	278.0
97	258.0
98	258.0
99	570.5
100	883.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12423460457908748
2	0.053923361347373085
3	0.012110162716002223
4	0.0024702321958014486
5	6.024956575125485E-5
6	3.0124782875627426E-4
7	6.024956575125485E-5
8	5.422460917612937E-4
9	4.819965260100388E-4
10	6.627452232638033E-4
11	0.0024702321958014486
12	0.0034342252478215267
13	0.014098398385793635
14	0.00957968095444952
15	0.023678079340243154
16	0.011266668795484656
17	0.021448845407446723
18	0.006868450495643053
19	0.007772193981911875
20	0.006567202666886779
21	0.006687701798389288
22	0.007711944416160621
23	0.00957968095444952
24	0.013917649688539872
25	0.01717112623910763
26	0.018617115817137746
27	0.009338682691444502
28	0.006145455706627994
29	0.011628166189992186
30	0.003614973945075291
31	0.006145455706627994
32	0.007229947890150582
33	0.008615687902429442
34	0.012712658373514773
35	0.01590588535833128
36	0.014399646214549908
37	0.010543674006469599
38	0.006386453969633015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1659763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.83385867469336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19834522585224	29.893966264779554
2	15.543142741678576	13.62631844598649
3	6.5373128278464145	8.596669398242392
4	3.454406464973151	6.056798591623207
5	1.9247466149194408	4.218453555148661
6	1.2175094609679762	3.2020882588303414
7	0.7426512003447514	2.278728743235225
8	0.502403367219226	1.7617822557102099
9	0.3611820355310944	1.424880207117726
>10	1.329035367570145	9.489104928258396
>50	0.08515070543711603	2.5949861808296304
>100	0.0863252093646712	8.21241361494618
>500	0.012134980542559556	3.6379907965315206
>1k	0.005240105234287081	3.634726723441222
>5k	4.1369251849634846E-4	1.3710920353192517
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	9243	0.5568867362388485	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	7310	0.44042432564167294	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6130	0.3693298380551922	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4034	0.24304674824056205	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3275	0.19731732783535963	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2434	0.1466474430385543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2173	0.1309223063774768	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1883	0.11344993230961288	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1832	0.11037720445629888	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1829	0.11019645575904512	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1818	0.10953371053578133	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1787	0.10766597399749242	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1759	0.10597898615645727	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1744	0.10507524267018846	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1672	0.10073727393609812	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1667	0.10043602610734183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	6.024956575125485E-5	0.0
16	0.0	0.0	0.0	1.204991315025097E-4	0.0
17	0.0	0.0	0.0	2.409982630050194E-4	0.0
18	0.0	0.0	0.0	3.614973945075291E-4	0.0
19	0.0	0.0	0.0	4.819965260100388E-4	0.0
20	0.0	0.0	0.0	7.229947890150582E-4	0.0
21	0.0	0.0	0.0	0.0010844921835225872	0.0
22	0.0	0.0	0.0	0.001626738275283881	0.0
23	0.0	0.0	0.0	0.0030124782875627426	0.0
24	0.0	0.0	0.0	0.00421746960258784	0.0
25	0.0	0.0	0.0	0.005121213088856662	0.0
26	0.0	0.0	0.0	0.006386453969633014	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	40	0.0044844756	19.999866	32
CATCGTT	345	0.0	18.086836	28
CGGCATC	375	0.0	17.919882	25
CGACGGA	45	0.008852217	17.77766	24
TAGCGTA	245	0.0	17.631474	7
TCGTTTA	360	0.0	17.33322	30
ATCGTTT	360	0.0	17.33322	29
CGCAAGA	455	0.0	17.234291	2
GTCCTAC	270	0.0	17.198027	1
GCATCGT	375	0.0	17.06604	27
TCTAGCG	415	0.0	16.578205	28
ATAGCGT	275	0.0	16.287365	6
CTAGCGG	425	0.0	16.188128	29
TAGCGGC	440	0.0	15.999894	30
TATTCCA	620	0.0	15.996521	5
AAGACGG	470	0.0	15.99652	5
CTATTCC	565	0.0	15.854958	4
CCTATTC	515	0.0	15.841214	3
GGCATCG	415	0.0	15.807126	26
CAAGACG	490	0.0	15.670061	4
>>END_MODULE