FastQCFastQC Report
Thu 2 Feb 2017
SRR4062748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659763
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA94090.5668881641535568No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC87520.5273041994549824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT72810.4386770882348866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT50100.3018503244137868No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG35890.21623569148125363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT31060.18713515122339755No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24810.1494791726288633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.13586277076907968No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC22080.1330310411787707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21410.12899432027343663No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20470.12333086109281867No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG20210.12176437238328604No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18880.11375118013836916No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18410.11091945054806018No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18390.11079895141655766No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17930.10802747139199995No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17860.10760572443174116No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16690.10055652523884434No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16610.1000745287128343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG400.00448071420.0027685
TACCGTC2900.019.8648197
ACCGTCG3050.019.4125238
CTAGCGG5150.019.2613229
TAGCGGC5300.019.01806330
TCTAGCG5300.018.71618828
CGTCGTA3250.017.72339410
ATACCGT3350.017.674096
CCGTCGT3350.017.1953759
AGCGGCG5900.016.81284731
CGGTCCA6000.016.5336310
GTCGTAG3750.015.36027511
GCGGCGC6500.015.26089232
TCGCGTA2100.015.2392859
GCCGGTC6650.015.1599928
AACCGCG2550.015.0609077
TACGCTA3300.015.0314789
TCTTGCG6600.015.0305722
CGCGTAA2250.014.933610
CGCCGGT6700.014.8080217
Produced by FastQC (version 0.11.5)