##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659763
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0508656958855	32.0	32.0	32.0	32.0	32.0
2	31.17494907405455	32.0	32.0	32.0	32.0	32.0
3	31.30754210089031	32.0	32.0	32.0	32.0	32.0
4	31.428175588924443	32.0	32.0	32.0	32.0	32.0
5	31.319524534526916	32.0	32.0	32.0	32.0	32.0
6	34.84912845990662	36.0	36.0	36.0	32.0	36.0
7	34.89002285266029	36.0	36.0	36.0	36.0	36.0
8	34.82484547492624	36.0	36.0	36.0	32.0	36.0
9	34.94934819007292	36.0	36.0	36.0	32.0	36.0
10	34.76744511113936	36.0	36.0	36.0	32.0	36.0
11	34.985171376877304	36.0	36.0	36.0	36.0	36.0
12	34.867522652330486	36.0	36.0	36.0	32.0	36.0
13	34.920590469844186	36.0	36.0	36.0	32.0	36.0
14	34.85609752717707	36.0	36.0	36.0	32.0	36.0
15	34.822786747264516	36.0	36.0	36.0	32.0	36.0
16	34.837212903288	36.0	36.0	36.0	32.0	36.0
17	34.78749255164743	36.0	36.0	36.0	32.0	36.0
18	34.78881623460699	36.0	36.0	36.0	32.0	36.0
19	34.77700972970237	36.0	36.0	36.0	32.0	36.0
20	34.76776383134219	36.0	36.0	36.0	32.0	36.0
21	34.7358219215635	36.0	36.0	36.0	32.0	36.0
22	34.7048132775583	36.0	36.0	36.0	32.0	36.0
23	34.617489966941065	36.0	36.0	36.0	32.0	36.0
24	34.59798718250738	36.0	36.0	36.0	32.0	36.0
25	34.57228170527961	36.0	36.0	36.0	32.0	36.0
26	34.48888244887975	36.0	36.0	36.0	32.0	36.0
27	34.454269073355654	36.0	36.0	36.0	32.0	36.0
28	34.40133079240832	36.0	36.0	36.0	32.0	36.0
29	34.34594276411753	36.0	36.0	36.0	32.0	36.0
30	34.3227352338858	36.0	36.0	36.0	32.0	36.0
31	34.30324208938264	36.0	36.0	36.0	32.0	36.0
32	34.269933719452716	36.0	36.0	36.0	32.0	36.0
33	34.211451273464945	36.0	36.0	36.0	32.0	36.0
34	34.18194404863827	36.0	36.0	36.0	32.0	36.0
35	34.11639613607485	36.0	36.0	36.0	32.0	36.0
36	34.07917094187544	36.0	36.0	36.0	32.0	36.0
37	34.04018947283438	36.0	36.0	36.0	32.0	36.0
38	33.573690942622534	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	39.0
22	149.0
23	464.0
24	1202.0
25	2999.0
26	6695.0
27	12898.0
28	22340.0
29	35963.0
30	53865.0
31	76926.0
32	107589.0
33	168235.0
34	390385.0
35	780000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.458371344454875	18.390071836500557	12.502696429625898	25.648860389418665
2	14.747225959368897	21.475596214640284	37.53752794826731	26.239649877723508
3	18.06607328877677	26.310262368783977	28.419720164866913	27.203944177572343
4	11.467929982605908	17.181470321896132	36.50162463345582	34.84897506204214
5	13.171760064539335	38.27853735744199	33.50809724038914	15.04160533762953
6	33.08066643329973	36.2109363938543	17.73152881649828	12.97686835634769
7	29.198689445918557	31.161327327631287	21.408387738920663	18.231595487529493
8	28.37328066603725	32.988738509405465	19.66311327103795	18.974867553519346
9	27.67219657504781	14.4275297613668	18.26472463128923	39.635549032296154
10	15.053893658683537	27.849495405934626	32.31854194908872	24.778068986293118
11	37.179585282749436	21.609160774484316	21.658567740263965	19.552686202502283
12	24.139844483352395	24.707638101458166	29.389043462962672	21.763473952226768
13	30.152491926543597	20.167313346507928	25.13604376536367	24.54415096158481
14	23.781768842901066	19.963814110809796	25.75000165686306	30.504415389426082
15	25.11485073471333	27.3125138950561	23.931549263358683	23.641086106871885
16	25.26077518296287	26.159578204840088	24.846860666251747	23.732785945945295
17	23.404425812600955	26.248325815191688	26.20392188523301	24.143326486974345
18	24.79757652146722	24.51524705635684	27.703473327215995	22.983703094959942
19	24.3980014014049	26.82039544199985	26.186991757256912	22.594611399338337
20	24.86698402121267	24.081389933382056	27.479585940884334	23.572040104520948
21	26.201213549677995	25.11533300918989	24.76281195040967	23.92064149072245
22	24.5829073186955	25.46502121085962	25.817842667898972	24.134228802545906
23	23.17485660577433	24.992348291319228	26.025027714850342	25.807767388056103
24	23.273865003617985	26.56053906491469	26.000519351256774	24.16507658021055
25	24.654588615221478	25.011447438183833	25.533209620668046	24.80075432592664
26	23.37815700193341	25.711261186085	26.684472421665024	24.22610939031657
27	25.046256659844403	25.657669989835885	25.680745601324528	23.61532774899519
28	23.642953843410176	24.833907009615228	26.787017182573656	24.736121964400944
29	23.146678170317088	25.854534653441487	27.270580197293228	23.7282069789482
30	24.116876927609542	25.434595180155238	27.263109251140072	23.185418641095143
31	23.71736205711297	25.506472912096484	25.955452676074835	24.820712354715706
32	23.389363421163143	25.95304269344479	25.969310076197626	24.688283809194445
33	23.005814685590654	25.30644435380232	26.54746490914667	25.140276051460358
34	23.451540973018435	26.061612410928547	27.088807257421692	23.398039358631323
35	25.074664274357243	25.30379337290927	26.469983967590554	23.15155838514294
36	23.66102871313555	26.247000324745155	26.285078050299955	23.80689291181934
37	24.94392271667702	26.039681568995093	25.94442700554236	23.07196870878553
38	23.450633495585805	26.056794992526022	25.933463834207256	24.559107677680917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	87.0
2	120.0
3	120.0
4	525.5
5	931.0
6	931.0
7	1334.5
8	1738.0
9	2008.5
10	2279.0
11	2279.0
12	2867.5
13	3456.0
14	4372.5
15	5289.0
16	5289.0
17	7838.0
18	10387.0
19	10387.0
20	12424.5
21	14462.0
22	14722.0
23	14982.0
24	14982.0
25	16980.5
26	18979.0
27	18979.0
28	25035.5
29	31092.0
30	38242.5
31	45393.0
32	45393.0
33	54694.0
34	63995.0
35	63995.0
36	71098.0
37	78201.0
38	88814.0
39	99427.0
40	99427.0
41	106823.5
42	114220.0
43	127588.0
44	140956.0
45	140956.0
46	160060.0
47	179164.0
48	179164.0
49	173131.0
50	167098.0
51	155695.0
52	144292.0
53	144292.0
54	132831.5
55	121371.0
56	121371.0
57	115222.0
58	109073.0
59	97169.5
60	85266.0
61	85266.0
62	80432.5
63	75599.0
64	62577.5
65	49556.0
66	49556.0
67	41184.0
68	32812.0
69	32812.0
70	26486.5
71	20161.0
72	15829.0
73	11497.0
74	11497.0
75	8648.0
76	5799.0
77	5799.0
78	5427.0
79	5055.0
80	3997.5
81	2940.0
82	2940.0
83	2754.0
84	2568.0
85	2568.0
86	1707.5
87	847.0
88	676.0
89	505.0
90	505.0
91	314.0
92	123.0
93	77.5
94	32.0
95	32.0
96	27.5
97	23.0
98	23.0
99	22.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009700180085952031
2	0.0
3	0.0
4	2.409982630050194E-4
5	0.0
6	1.204991315025097E-4
7	4.2174696025878397E-4
8	0.0021689843670451747
9	0.003675223510826546
10	7.83244354766313E-4
11	0.004699466128597878
12	3.0124782875627426E-4
13	1.8074869725376454E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.409982630050194E-4
22	0.0
23	1.8074869725376454E-4
24	0.0
25	1.8074869725376454E-4
26	0.0
27	1.204991315025097E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.409982630050194E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1659763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.12139926478431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.18702169461545	26.805815459864384
2	16.87832055090383	13.881203165854613
3	7.171016680988012	8.846467202200094
4	3.801341479850985	6.252659229389536
5	2.141620715633087	4.403322026064065
6	1.30993191928939	3.231974007767053
7	0.8361799356015801	2.4069422292351947
8	0.5836418067702461	1.9200134211055506
9	0.42511044741464776	1.5733022795791942
>10	1.4596248291907659	9.718024725907325
>50	0.08977259782685229	2.592390311411741
>100	0.09551834223660134	8.44180035005703
>500	0.013981725137091571	3.973859716895233
>1k	0.006328570325209869	4.1092448646495265
>5k	5.887042162985924E-4	1.8429810100195496
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	9409	0.5668881641535568	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	8752	0.5273041994549824	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7281	0.4386770882348866	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5010	0.3018503244137868	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3589	0.21623569148125363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3106	0.18713515122339755	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2481	0.1494791726288633	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2255	0.13586277076907968	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	2208	0.1330310411787707	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	2141	0.12899432027343663	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2047	0.12333086109281867	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	2021	0.12176437238328604	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1888	0.11375118013836916	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1841	0.11091945054806018	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1839	0.11079895141655766	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1793	0.10802747139199995	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1786	0.10760572443174116	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1669	0.10055652523884434	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1661	0.1000745287128343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	6.024956575125485E-5	0.0
16	0.0	0.0	0.0	1.204991315025097E-4	0.0
17	0.0	0.0	0.0	2.409982630050194E-4	0.0
18	0.0	0.0	0.0	3.614973945075291E-4	0.0
19	0.0	0.0	0.0	4.819965260100388E-4	0.0
20	0.0	0.0	0.0	9.037434862688227E-4	0.0
21	0.0	0.0	0.0	0.0012652408807763518	0.0
22	0.0	0.0	0.0	0.00198823566979141	0.0
23	0.0	0.0	0.0	0.003313726116319017	0.0
24	0.0	0.0	0.0	0.004578966997095368	0.0
25	0.0	0.0	0.0	0.005542960049115446	0.0
26	0.0	0.0	0.0	0.006747951364140543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGCG	40	0.004480714	20.002768	5
TACCGTC	290	0.0	19.864819	7
ACCGTCG	305	0.0	19.412523	8
CTAGCGG	515	0.0	19.26132	29
TAGCGGC	530	0.0	19.018063	30
TCTAGCG	530	0.0	18.716188	28
CGTCGTA	325	0.0	17.723394	10
ATACCGT	335	0.0	17.67409	6
CCGTCGT	335	0.0	17.195375	9
AGCGGCG	590	0.0	16.812847	31
CGGTCCA	600	0.0	16.53363	10
GTCGTAG	375	0.0	15.360275	11
GCGGCGC	650	0.0	15.260892	32
TCGCGTA	210	0.0	15.239285	9
GCCGGTC	665	0.0	15.159992	8
AACCGCG	255	0.0	15.060907	7
TACGCTA	330	0.0	15.031478	9
TCTTGCG	660	0.0	15.030572	2
CGCGTAA	225	0.0	14.9336	10
CGCCGGT	670	0.0	14.808021	7
>>END_MODULE