FastQCFastQC Report
Thu 2 Feb 2017
SRR4062747_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062747_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811585
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24450.13496468562060296No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24300.13413668141434157No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23930.1320942710388969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23800.13137666739347037No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23710.13087986486971354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22890.12635344187548472No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22480.12409023037837032No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22110.1220478200029256No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20730.11443018130532104No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20020.1105109613956839No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19670.10857895158107403No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19310.10659174148604676No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18620.10278292213724445No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18140.10013330867720807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTC3000.017.5979653
ATCGTTT5000.017.27895729
CATCGTT5200.017.22972928
TCTAGCG5450.016.73293528
AAGACGG6450.016.3688475
GTATCAA25500.016.1410031
GTACTAA805.5776643E-516.0153921
CTAGCGG5700.015.99903429
CAAGACG6300.015.9968274
GTATAAT1850.015.5825421
CGTTTAT5600.015.42721531
TAGCGGC5950.015.32638230
GCATCGT5850.015.31531427
CGGCATC6000.015.19992225
CTATAGA1901.8189894E-1215.1724761
GCGCAAG6500.015.0298291
CGTCGTA5150.014.91295510
GACGGAC7200.014.8867567
ACCGTCG4950.014.8665578
TCGTTTA5850.014.76793130
Produced by FastQC (version 0.11.5)