##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062747_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1811585
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273805534932116	32.0	32.0	32.0	32.0	32.0
2	30.874712475539376	32.0	32.0	32.0	32.0	32.0
3	30.918804251525597	32.0	32.0	32.0	32.0	32.0
4	30.999767606819443	32.0	32.0	32.0	32.0	32.0
5	30.862682678428005	32.0	32.0	32.0	32.0	32.0
6	34.53129883499808	36.0	36.0	36.0	32.0	36.0
7	34.47966228468441	36.0	36.0	36.0	32.0	36.0
8	34.465211403273926	36.0	36.0	36.0	32.0	36.0
9	34.617335648065094	36.0	36.0	36.0	32.0	36.0
10	34.30932470736951	36.0	36.0	36.0	32.0	36.0
11	34.59384792874748	36.0	36.0	36.0	32.0	36.0
12	34.396234788872725	36.0	36.0	36.0	32.0	36.0
13	34.49949574543839	36.0	36.0	36.0	32.0	36.0
14	34.390252182481085	36.0	36.0	36.0	32.0	36.0
15	34.32969361084354	36.0	36.0	36.0	32.0	36.0
16	34.34013639989291	36.0	36.0	36.0	32.0	36.0
17	34.266881211756555	36.0	36.0	36.0	32.0	36.0
18	34.28680740898164	36.0	36.0	36.0	32.0	36.0
19	34.2806801778553	36.0	36.0	36.0	32.0	36.0
20	34.27510053351071	36.0	36.0	36.0	32.0	36.0
21	34.25364694452648	36.0	36.0	36.0	32.0	36.0
22	34.22903203548274	36.0	36.0	36.0	32.0	36.0
23	34.16923964373739	36.0	36.0	36.0	32.0	36.0
24	34.15216785301269	36.0	36.0	36.0	32.0	36.0
25	34.13302715577795	36.0	36.0	36.0	32.0	36.0
26	34.07425486521471	36.0	36.0	36.0	32.0	36.0
27	34.08103235564437	36.0	36.0	36.0	32.0	36.0
28	34.058959419513855	36.0	36.0	36.0	32.0	36.0
29	34.027246306411236	36.0	36.0	36.0	32.0	36.0
30	34.01191663653651	36.0	36.0	36.0	32.0	36.0
31	34.031459191812694	36.0	36.0	36.0	32.0	36.0
32	33.98522233292945	36.0	36.0	36.0	32.0	36.0
33	33.94142035841542	36.0	36.0	36.0	32.0	36.0
34	33.9602094298639	36.0	36.0	36.0	32.0	36.0
35	33.91812032005122	36.0	36.0	36.0	32.0	36.0
36	33.87860961533685	36.0	36.0	36.0	32.0	36.0
37	33.892544926128224	36.0	36.0	36.0	32.0	36.0
38	33.28011658299224	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	2.0
9	10.0
10	12.0
11	17.0
12	5.0
13	7.0
14	125.0
15	336.0
16	505.0
17	590.0
18	747.0
19	956.0
20	1371.0
21	2020.0
22	3044.0
23	4764.0
24	7313.0
25	11234.0
26	16773.0
27	24478.0
28	34541.0
29	47349.0
30	64575.0
31	88697.0
32	121709.0
33	183293.0
34	402633.0
35	794473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20496391896716	17.202899303579144	11.261438002407244	26.330698775046447
2	17.09604356895629	19.50225527118207	37.10888980568824	26.292811354173395
3	18.619564587318543	23.75872605768514	28.483122030932133	29.13858732406418
4	12.712312100789216	15.24805097091264	35.88984639061839	36.149790537679756
5	14.98813190695415	36.097108601331435	33.51637796840327	15.39838152331114
6	34.513889847232385	35.31065509294399	16.74570470446987	13.429750355353768
7	30.57611460467745	30.137603334981982	21.099766833886807	18.186515226453757
8	28.26738441989005	32.42077767516625	19.537983162754703	19.773854742188995
9	27.62080614712019	14.189105642588236	18.44925424215326	39.74083396813831
10	16.084172013760394	25.851689357774667	31.230983408921293	26.83315521954365
11	37.80344146897246	20.754910706399816	22.419395082227837	19.022252742399886
12	25.273054668343324	23.28226450173388	28.30881094669624	23.135869883226555
13	29.359842899187942	18.854913071730522	25.27459241073228	26.51065161834926
14	23.9919201242786	19.6569089120433	24.479053380397854	31.872117583280247
15	25.535069082492623	26.564956978038477	21.920992420360783	25.978981519108114
16	26.557773811502038	25.18357520193134	23.003473036900534	25.255177949666084
17	24.52291720037809	25.4863031245307	24.808968118656196	25.18181155643501
18	25.73818862320901	24.127364414475334	25.50837843880331	24.626068523512348
19	26.036070352091684	24.61815331279741	24.850286509224603	24.495489825886303
20	26.05321281316227	23.894095494239245	24.204394084177533	25.848297608420957
21	27.459098519613995	23.848639267077267	23.863047319688192	24.829214893620545
22	26.341811067481263	23.68327931586336	24.356551736701682	25.618357879953695
23	24.744978795617577	23.517653021003433	25.09873480334191	26.638633380037074
24	25.370637617054353	24.683907696962134	24.302642576923798	25.642812109059708
25	25.367420536682427	23.96741302816357	24.632082576040315	26.033083859113688
26	25.27087751339826	24.781489838746097	25.104858928371286	24.842773719484352
27	26.017979145780796	24.233589778694427	24.01519902485989	25.73323205066489
28	25.06940415381583	24.04234964457455	25.12526973636634	25.76297646524327
29	25.08533468919293	24.444006476769033	24.951791352199436	25.518867481838605
30	25.020355687696554	24.469496277462238	25.335061519718383	25.175086515122825
31	25.827353935803405	24.304801882199154	23.908996863369435	25.95884731862801
32	25.23795696514749	24.368659127268472	23.916482929258244	26.476900978325794
33	24.95956260261162	23.91227541373116	24.72488768616384	26.40327429749338
34	25.931998657374283	23.942074975046594	24.88610868201853	25.23981768556059
35	26.60185877341272	23.81817057564117	24.724314333004656	24.855656317941456
36	25.3363572823767	24.545110245688498	24.21474307658843	25.903789395346372
37	26.297998866084843	24.317622112560127	24.26158881718958	25.122790204165458
38	25.24584734113906	24.10286559682913	24.872011438097918	25.779275623933888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	152.0
2	159.0
3	159.0
4	372.0
5	585.0
6	585.0
7	623.0
8	661.0
9	662.5
10	664.0
11	664.0
12	916.0
13	1168.0
14	1780.5
15	2393.0
16	2393.0
17	3508.5
18	4624.0
19	4624.0
20	5926.5
21	7229.0
22	8730.5
23	10232.0
24	10232.0
25	12631.5
26	15031.0
27	15031.0
28	19234.5
29	23438.0
30	29223.0
31	35008.0
32	35008.0
33	46665.0
34	58322.0
35	58322.0
36	65352.0
37	72382.0
38	83691.5
39	95001.0
40	95001.0
41	102125.5
42	109250.0
43	124691.5
44	140133.0
45	140133.0
46	143838.5
47	147544.0
48	147544.0
49	159007.5
50	170471.0
51	173387.0
52	176303.0
53	176303.0
54	168483.5
55	160664.0
56	160664.0
57	156507.0
58	152350.0
59	137459.0
60	122568.0
61	122568.0
62	116551.5
63	110535.0
64	91037.5
65	71540.0
66	71540.0
67	59660.5
68	47781.0
69	47781.0
70	38429.5
71	29078.0
72	22556.5
73	16035.0
74	16035.0
75	12117.0
76	8199.0
77	8199.0
78	8012.5
79	7826.0
80	6221.5
81	4617.0
82	4617.0
83	4724.0
84	4831.0
85	4831.0
86	3184.5
87	1538.0
88	1330.0
89	1122.0
90	1122.0
91	844.0
92	566.0
93	484.5
94	403.0
95	403.0
96	363.5
97	324.0
98	324.0
99	594.5
100	865.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11299497401446801
2	0.0517778630315442
3	0.011040056083484904
4	0.00231841177753183
5	2.760014020871226E-4
6	5.520028041742452E-4
7	5.5200280417424524E-5
8	3.3120168250454717E-4
9	2.760014020871226E-4
10	4.968025237568208E-4
11	0.0018216092537750093
12	0.0023736120579492545
13	0.014572874030200075
14	0.009439247951379593
15	0.023018516934066025
16	0.011150456644319753
17	0.020368903474029646
18	0.004968025237568207
19	0.006900035052178065
20	0.0057408291634121495
21	0.005409627480907603
22	0.007396837575934886
23	0.009218446829709894
24	0.013634469263103856
25	0.01705688664898418
26	0.018878495902759185
27	0.008445642903865952
28	0.005188826359237906
29	0.010377652718475811
30	0.0028704145817060748
31	0.004802424396315934
32	0.006789634491343215
33	0.007893640099691707
34	0.011536858607241724
35	0.01562167935813114
36	0.014738474871452347
37	0.008942445427622773
38	0.005244026639655329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1811585.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.778038291585304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53639824061344	43.848675467416584
2	10.938781503244172	11.765324210895004
3	3.234856535116657	5.218927158398657
4	1.372630428003948	2.952694868695658
5	0.7710863116926879	2.073375459816333
6	0.45121777101043936	1.4559363940345909
7	0.3101736885883315	1.167637275136188
8	0.21611934807571387	0.9297979661094555
9	0.15832225020421153	0.7662834030502834
>10	0.8416642871726769	7.942580861454641
>50	0.07030837028395805	2.7141958675543796
>100	0.0791051827003387	9.353551588120247
>500	0.013338795427050864	4.884267748653266
>1k	0.00599728786642597	4.926751730664663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2445	0.13496468562060296	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2430	0.13413668141434157	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2393	0.1320942710388969	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2380	0.13137666739347037	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2371	0.13087986486971354	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2289	0.12635344187548472	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2248	0.12409023037837032	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2211	0.1220478200029256	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2073	0.11443018130532104	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2002	0.1105109613956839	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1967	0.10857895158107403	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1931	0.10659174148604676	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1862	0.10278292213724445	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1814	0.10013330867720807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1040056083484905E-4
2	0.0	0.0	0.0	0.0	1.1040056083484905E-4
3	0.0	0.0	0.0	0.0	1.1040056083484905E-4
4	0.0	0.0	0.0	0.0	1.1040056083484905E-4
5	0.0	0.0	0.0	0.0	1.1040056083484905E-4
6	0.0	0.0	0.0	0.0	1.6560084125227356E-4
7	0.0	0.0	0.0	5.5200280417424524E-5	1.6560084125227356E-4
8	0.0	0.0	0.0	5.5200280417424524E-5	1.6560084125227356E-4
9	0.0	0.0	0.0	5.5200280417424524E-5	1.6560084125227356E-4
10	0.0	0.0	0.0	1.1040056083484905E-4	1.6560084125227356E-4
11	0.0	0.0	0.0	1.1040056083484905E-4	1.6560084125227356E-4
12	0.0	0.0	0.0	1.1040056083484905E-4	3.864019629219716E-4
13	0.0	0.0	0.0	1.1040056083484905E-4	3.864019629219716E-4
14	0.0	0.0	0.0	1.1040056083484905E-4	3.864019629219716E-4
15	0.0	0.0	0.0	1.1040056083484905E-4	5.520028041742452E-4
16	0.0	0.0	0.0	1.6560084125227356E-4	6.624033650090942E-4
17	0.0	0.0	0.0	2.208011216696981E-4	6.624033650090942E-4
18	0.0	0.0	0.0	2.760014020871226E-4	7.176036454265188E-4
19	0.0	0.0	0.0	2.760014020871226E-4	7.728039258439433E-4
20	0.0	0.0	0.0	3.312016825045471E-4	8.280042062613678E-4
21	0.0	0.0	0.0	3.864019629219716E-4	8.280042062613678E-4
22	0.0	0.0	0.0	5.520028041742452E-4	8.280042062613678E-4
23	0.0	0.0	0.0	0.0010488053279310659	8.280042062613678E-4
24	0.0	0.0	0.0	0.0014904075712704622	8.280042062613678E-4
25	0.0	0.0	0.0	0.0019320098146098582	8.280042062613678E-4
26	0.0	0.0	0.0	0.0021528109362795563	8.280042062613678E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATTC	300	0.0	17.597965	3
ATCGTTT	500	0.0	17.278957	29
CATCGTT	520	0.0	17.229729	28
TCTAGCG	545	0.0	16.732935	28
AAGACGG	645	0.0	16.368847	5
GTATCAA	2550	0.0	16.141003	1
GTACTAA	80	5.5776643E-5	16.015392	1
CTAGCGG	570	0.0	15.999034	29
CAAGACG	630	0.0	15.996827	4
GTATAAT	185	0.0	15.582542	1
CGTTTAT	560	0.0	15.427215	31
TAGCGGC	595	0.0	15.326382	30
GCATCGT	585	0.0	15.315314	27
CGGCATC	600	0.0	15.199922	25
CTATAGA	190	1.8189894E-12	15.172476	1
GCGCAAG	650	0.0	15.029829	1
CGTCGTA	515	0.0	14.912955	10
GACGGAC	720	0.0	14.886756	7
ACCGTCG	495	0.0	14.866557	8
TCGTTTA	585	0.0	14.767931	30
>>END_MODULE