FastQCFastQC Report
Thu 2 Feb 2017
SRR4062747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811585
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25810.1424719237573727No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24810.1369518957156302No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24490.13518548674227265No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24200.13358467861016735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24100.1330326758059931No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23470.12955505813969534No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22840.1260774404733976No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA22820.12596703991256275No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22390.12359342785461351No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21830.12050221215123773No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21680.11967420794497637No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19930.11001415887192706No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19410.10714374429022099No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19190.10592933812103766No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG19110.10548773587769826No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18940.10454933111060205No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18770.10361092634350581No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18570.10250692073515734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22050.017.9987411
TCTAGCG5600.017.71359828
CTAGCGG5700.016.84145229
ACCGTCG5400.016.593788
TACCGTC5450.016.4427
CATCGTT5450.016.14616228
CCCTTAT1102.0168773E-716.0029131
TAGCGGC6000.015.99937830
ATCGTTT5400.015.99937829
AACCGCG4450.015.8217947
CGTCGTA5900.015.1862710
CCGTCGT5800.015.1726639
ATACCGT5950.015.0603186
GCATCGT5850.015.04215127
GTATTAA2350.014.9814491
AGTATCG650.00416082814.7702888
CGTTTAT5700.014.59592431
AGCGGCG7100.014.42197631
CGCGGTC5000.014.07984310
CGAGCCG8000.013.99945615
Produced by FastQC (version 0.11.5)