##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1811585
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145458258927956	32.0	32.0	32.0	32.0	32.0
2	31.1322399997792	32.0	32.0	32.0	32.0	32.0
3	31.26603940748019	32.0	32.0	32.0	32.0	32.0
4	31.399707990516593	32.0	32.0	32.0	32.0	32.0
5	31.281869191895495	32.0	32.0	32.0	32.0	32.0
6	34.81767016176442	36.0	36.0	36.0	32.0	36.0
7	34.84652058832459	36.0	36.0	36.0	32.0	36.0
8	34.76872628112951	36.0	36.0	36.0	32.0	36.0
9	34.92674812388047	36.0	36.0	36.0	32.0	36.0
10	34.71172647157047	36.0	36.0	36.0	32.0	36.0
11	34.94216445819545	36.0	36.0	36.0	36.0	36.0
12	34.80997965869667	36.0	36.0	36.0	32.0	36.0
13	34.88465901406779	36.0	36.0	36.0	32.0	36.0
14	34.82690185666143	36.0	36.0	36.0	32.0	36.0
15	34.7846333459374	36.0	36.0	36.0	32.0	36.0
16	34.804594319339145	36.0	36.0	36.0	32.0	36.0
17	34.76239646497404	36.0	36.0	36.0	32.0	36.0
18	34.76976515040696	36.0	36.0	36.0	32.0	36.0
19	34.76502565433032	36.0	36.0	36.0	32.0	36.0
20	34.75581051951744	36.0	36.0	36.0	32.0	36.0
21	34.75552403006207	36.0	36.0	36.0	32.0	36.0
22	34.73456282757916	36.0	36.0	36.0	32.0	36.0
23	34.66809285791172	36.0	36.0	36.0	32.0	36.0
24	34.635351363584924	36.0	36.0	36.0	32.0	36.0
25	34.62370410441685	36.0	36.0	36.0	32.0	36.0
26	34.55179966714231	36.0	36.0	36.0	32.0	36.0
27	34.53778486794713	36.0	36.0	36.0	32.0	36.0
28	34.50982095789047	36.0	36.0	36.0	32.0	36.0
29	34.47051173419961	36.0	36.0	36.0	32.0	36.0
30	34.44794641156777	36.0	36.0	36.0	32.0	36.0
31	34.45159294209215	36.0	36.0	36.0	32.0	36.0
32	34.42360363990649	36.0	36.0	36.0	32.0	36.0
33	34.38687061330272	36.0	36.0	36.0	32.0	36.0
34	34.38709914246365	36.0	36.0	36.0	32.0	36.0
35	34.359733051443904	36.0	36.0	36.0	32.0	36.0
36	34.32204174797208	36.0	36.0	36.0	32.0	36.0
37	34.32915485610667	36.0	36.0	36.0	32.0	36.0
38	33.860257178106465	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	28.0
22	127.0
23	432.0
24	1213.0
25	3026.0
26	6607.0
27	12829.0
28	22241.0
29	35555.0
30	54007.0
31	78643.0
32	115039.0
33	186891.0
34	441601.0
35	853340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41516866896542	18.60420951983474	12.026048101542548	26.954573709657296
2	16.02132938835329	20.688292296524864	36.19289185989064	27.09748645523119
3	17.585318933420183	24.7041126968925	28.836736890623406	28.873831479063917
4	12.168093964280944	16.251596670755177	36.047830018183	35.53247934678088
5	14.554950007810849	36.63078092474924	33.106183928641414	15.708085138798497
6	34.48247690829171	35.96703652776221	16.316079711588937	13.23440685235714
7	30.311860709282186	30.411111196978545	21.039226574842484	18.237801518896784
8	28.16094143052793	32.753109093307856	19.20917053492027	19.87677894124395
9	27.615268095893164	14.009702370831945	18.127137712280764	40.24789182099413
10	15.762805799635895	26.20732826146718	31.234426423164095	26.795439515732834
11	38.06606216043447	20.711220387898248	22.21295430431917	19.009763147348117
12	25.107268300195575	23.395888338294935	28.156707491089268	23.340135870420227
13	29.320450302553237	18.914904210794763	25.24169482805636	26.522950658595633
14	23.84642390305942	19.56177577192115	24.438226436091288	32.15357388892814
15	25.466594170298386	26.811659403229772	21.707344673310942	26.014401753160904
16	26.500274621395075	25.33107748187361	22.81565590353199	25.352991993199325
17	24.585708095397123	25.659077548113945	24.554575137241695	25.200639219247233
18	25.803371081125093	24.159175528611684	25.385670559206442	24.65178283105678
19	26.053593952257277	24.717360764192684	24.75169533861232	24.47734994493772
20	26.140037591390964	23.831451463773437	24.12649696260457	25.902013982231033
21	27.503223698155864	23.952518900586448	23.635282713912247	24.908974687345438
22	26.161966544195575	23.912774676746977	24.303427276902422	25.62183150215502
23	24.7064999470077	23.431703967356743	25.070932399491276	26.790863686144277
24	25.345705556184228	24.68076297827593	24.36540377625118	25.60812768928866
25	25.319223397866175	24.030240938316965	24.613929025648275	26.036606638168585
26	25.409903482309694	24.75125373636898	24.930213045482272	24.908629735839057
27	26.118951084271508	24.23215029932352	23.867166045203508	25.78173257120146
28	25.0209071062081	24.090395979211575	25.131583668445035	25.757113246135287
29	25.244854643861594	24.34045324950251	24.946828329887914	25.467863776747983
30	25.147867751168175	24.40730078908801	25.21747530477455	25.227356154969264
31	25.80640709654805	24.296789827692326	23.91772950206587	25.979073573693757
32	25.376562512937568	24.295299420121054	23.903046227474835	26.425091839466546
33	24.951906755686316	23.931639972731062	24.711951136711775	26.404502134870846
34	25.761032466044924	23.87897890521284	24.974373269816212	25.38561535892602
35	26.59207268772925	23.808764148521874	24.64924361815758	24.949919545591293
36	25.43220439559833	24.48596118868284	24.20085174032684	25.88098267539199
37	26.4119541727272	24.376995835138842	24.11363529726731	25.09741469486665
38	25.241128891141063	24.042784680580464	24.848599263737846	25.867487164540627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	83.5
2	93.0
3	93.0
4	286.5
5	480.0
6	480.0
7	533.0
8	586.0
9	594.5
10	603.0
11	603.0
12	853.5
13	1104.0
14	1677.5
15	2251.0
16	2251.0
17	3471.0
18	4691.0
19	4691.0
20	6000.0
21	7309.0
22	8996.0
23	10683.0
24	10683.0
25	12893.5
26	15104.0
27	15104.0
28	19306.5
29	23509.0
30	29306.5
31	35104.0
32	35104.0
33	47288.5
34	59473.0
35	59473.0
36	66080.0
37	72687.0
38	83771.5
39	94856.0
40	94856.0
41	102253.0
42	109650.0
43	125537.0
44	141424.0
45	141424.0
46	143888.5
47	146353.0
48	146353.0
49	158997.5
50	171642.0
51	173468.0
52	175294.0
53	175294.0
54	168276.5
55	161259.0
56	161259.0
57	156923.5
58	152588.0
59	137645.0
60	122702.0
61	122702.0
62	116665.0
63	110628.0
64	90808.0
65	70988.0
66	70988.0
67	59576.0
68	48164.0
69	48164.0
70	38926.0
71	29688.0
72	22981.5
73	16275.0
74	16275.0
75	12120.0
76	7965.0
77	7965.0
78	7909.5
79	7854.0
80	6167.5
81	4481.0
82	4481.0
83	4223.5
84	3966.0
85	3966.0
86	2513.0
87	1060.0
88	896.5
89	733.0
90	733.0
91	445.0
92	157.0
93	107.0
94	57.0
95	57.0
96	39.5
97	22.0
98	22.0
99	25.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009218446829709894
2	0.0
3	0.0
4	1.6560084125227359E-4
5	1.1040056083484905E-4
6	2.208011216696981E-4
7	3.864019629219717E-4
8	0.002428812338366679
9	0.003808819348802292
10	0.0012696064496007641
11	0.004360822152976537
12	3.3120168250454717E-4
13	1.6560084125227359E-4
14	5.5200280417424524E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.5200280417424524E-5
22	5.5200280417424524E-5
23	5.5200280417424524E-5
24	0.0
25	5.5200280417424524E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1040056083484905E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1811585.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.13299165066314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46711896659079	41.94991641238195
2	11.60693295690881	12.102082778646677
3	3.312307270278526	5.180414617975837
4	1.5137613630772977	3.1566763400962086
5	0.8061450505906039	2.101337659583168
6	0.4900298880043209	1.5328034435942792
7	0.33156111725291415	1.2099691070201501
8	0.2254272356901319	0.9401756956852575
9	0.17117327657424644	0.803139749861566
>10	0.8967935347677413	8.262448228878801
>50	0.07498628933870773	2.766259586087279
>100	0.08307492118319935	9.583531095074731
>500	0.013649900036386746	4.865300659907169
>1k	0.007038229706261915	5.545944625206933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2581	0.1424719237573727	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2481	0.1369518957156302	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2449	0.13518548674227265	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2420	0.13358467861016735	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2410	0.1330326758059931	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2347	0.12955505813969534	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2284	0.1260774404733976	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2282	0.12596703991256275	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2239	0.12359342785461351	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2183	0.12050221215123773	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2168	0.11967420794497637	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1993	0.11001415887192706	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1941	0.10714374429022099	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1919	0.10592933812103766	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1911	0.10548773587769826	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1894	0.10454933111060205	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1877	0.10361092634350581	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1857	0.10250692073515734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5200280417424524E-5
2	0.0	0.0	0.0	0.0	5.5200280417424524E-5
3	0.0	0.0	0.0	0.0	5.5200280417424524E-5
4	0.0	0.0	0.0	0.0	5.5200280417424524E-5
5	0.0	0.0	0.0	0.0	5.5200280417424524E-5
6	0.0	0.0	0.0	0.0	5.5200280417424524E-5
7	0.0	0.0	0.0	0.0	5.5200280417424524E-5
8	0.0	0.0	0.0	0.0	5.5200280417424524E-5
9	0.0	0.0	0.0	0.0	5.5200280417424524E-5
10	0.0	0.0	0.0	0.0	5.5200280417424524E-5
11	0.0	0.0	0.0	0.0	5.5200280417424524E-5
12	0.0	0.0	0.0	0.0	3.312016825045471E-4
13	0.0	0.0	0.0	0.0	3.864019629219716E-4
14	0.0	0.0	0.0	0.0	3.864019629219716E-4
15	0.0	0.0	0.0	0.0	4.968025237568206E-4
16	0.0	0.0	0.0	5.5200280417424524E-5	4.968025237568206E-4
17	0.0	0.0	0.0	1.1040056083484905E-4	5.520028041742452E-4
18	0.0	0.0	0.0	1.1040056083484905E-4	5.520028041742452E-4
19	0.0	0.0	0.0	1.6560084125227356E-4	5.520028041742452E-4
20	0.0	0.0	0.0	2.208011216696981E-4	6.624033650090942E-4
21	0.0	0.0	0.0	2.760014020871226E-4	6.624033650090942E-4
22	0.0	0.0	0.0	3.864019629219716E-4	7.176036454265188E-4
23	0.0	0.0	0.0	8.280042062613678E-4	7.728039258439433E-4
24	0.0	0.0	0.0	0.001159205888765915	8.280042062613678E-4
25	0.0	0.0	0.0	0.0014904075712704622	8.280042062613678E-4
26	0.0	0.0	0.0	0.0017664089733575848	8.280042062613678E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2205	0.0	17.998741	1
TCTAGCG	560	0.0	17.713598	28
CTAGCGG	570	0.0	16.841452	29
ACCGTCG	540	0.0	16.59378	8
TACCGTC	545	0.0	16.442	7
CATCGTT	545	0.0	16.146162	28
CCCTTAT	110	2.0168773E-7	16.002913	1
TAGCGGC	600	0.0	15.999378	30
ATCGTTT	540	0.0	15.999378	29
AACCGCG	445	0.0	15.821794	7
CGTCGTA	590	0.0	15.18627	10
CCGTCGT	580	0.0	15.172663	9
ATACCGT	595	0.0	15.060318	6
GCATCGT	585	0.0	15.042151	27
GTATTAA	235	0.0	14.981449	1
AGTATCG	65	0.004160828	14.770288	8
CGTTTAT	570	0.0	14.595924	31
AGCGGCG	710	0.0	14.421976	31
CGCGGTC	500	0.0	14.079843	10
CGAGCCG	800	0.0	13.999456	15
>>END_MODULE