FastQCFastQC Report
Thu 2 Feb 2017
SRR4062746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523724
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35660.23403188503954786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23320.15304608971178507No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19580.12850096211649878No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18240.11970671853957804No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17700.11616276963544579No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17200.11288133546495298No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16640.10920612919400102No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16240.10658098185760677No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15950.10467775003872092No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15700.10303703295347451No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15480.10159320191845767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC401.5951705E-423.9971458
GACGCTA350.002067094222.85667223
GTACTAA601.13066955E-418.6834551
TATACTG1800.017.7744965
CTAGCGG3800.017.68384729
TCTAGCG3850.017.45304128
GTCCTAA5250.017.0820161
TAGCGGC4150.015.80690530
TAGGACG9000.015.4638124
TTAGGAC6950.015.4236373
TCTATAC1357.921699E-915.4065863
GTCCTAT4900.015.360741
ACCGTCG3550.015.3221218
CATCGTT3350.015.28226728
GTCTTAG1603.0013325E-1015.013491
TACCGTC3750.014.9315587
CGCCGGT4650.014.793947
CGGTCCA4900.014.69454110
CTATTCC4600.014.2582384
AGGACGT9850.014.1293725
Produced by FastQC (version 0.11.5)