##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062746_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523724
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27966482118809	32.0	32.0	32.0	32.0	32.0
2	30.950508097266958	32.0	32.0	32.0	32.0	32.0
3	30.99238444757712	32.0	32.0	32.0	32.0	32.0
4	31.05104992767719	32.0	32.0	32.0	32.0	32.0
5	30.990107788549633	32.0	32.0	32.0	32.0	32.0
6	34.63829210539441	36.0	36.0	36.0	32.0	36.0
7	34.57737621774022	36.0	36.0	36.0	32.0	36.0
8	34.56637947554807	36.0	36.0	36.0	32.0	36.0
9	34.68005098036127	36.0	36.0	36.0	32.0	36.0
10	34.43843570095372	36.0	36.0	36.0	32.0	36.0
11	34.66325791285036	36.0	36.0	36.0	32.0	36.0
12	34.50331293593853	36.0	36.0	36.0	32.0	36.0
13	34.58084141222426	36.0	36.0	36.0	32.0	36.0
14	34.49034930210458	36.0	36.0	36.0	32.0	36.0
15	34.442511898480305	36.0	36.0	36.0	32.0	36.0
16	34.46168006804382	36.0	36.0	36.0	32.0	36.0
17	34.39367365743402	36.0	36.0	36.0	32.0	36.0
18	34.416335241815446	36.0	36.0	36.0	32.0	36.0
19	34.39053332493286	36.0	36.0	36.0	32.0	36.0
20	34.36530500274328	36.0	36.0	36.0	32.0	36.0
21	34.34329445490128	36.0	36.0	36.0	32.0	36.0
22	34.31730943399198	36.0	36.0	36.0	32.0	36.0
23	34.272345910414224	36.0	36.0	36.0	32.0	36.0
24	34.25371195833366	36.0	36.0	36.0	32.0	36.0
25	34.23649230438058	36.0	36.0	36.0	32.0	36.0
26	34.1834177318202	36.0	36.0	36.0	32.0	36.0
27	34.1816451010813	36.0	36.0	36.0	32.0	36.0
28	34.162405396252865	36.0	36.0	36.0	32.0	36.0
29	34.13098697664407	36.0	36.0	36.0	32.0	36.0
30	34.09566955695389	36.0	36.0	36.0	32.0	36.0
31	34.11047407535748	36.0	36.0	36.0	32.0	36.0
32	34.055289540625466	36.0	36.0	36.0	32.0	36.0
33	34.03157724102265	36.0	36.0	36.0	32.0	36.0
34	34.023217459329906	36.0	36.0	36.0	32.0	36.0
35	33.983265998304155	36.0	36.0	36.0	32.0	36.0
36	33.93786210626072	36.0	36.0	36.0	32.0	36.0
37	33.93267875284501	36.0	36.0	36.0	32.0	36.0
38	33.337567039700104	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	8.0
8	4.0
9	3.0
10	8.0
11	12.0
12	17.0
13	6.0
14	171.0
15	404.0
16	517.0
17	566.0
18	749.0
19	916.0
20	1291.0
21	1824.0
22	2705.0
23	3942.0
24	5843.0
25	8656.0
26	12833.0
27	18960.0
28	27030.0
29	36888.0
30	51116.0
31	69418.0
32	94947.0
33	144743.0
34	330702.0
35	709444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.02492923356734	17.28475045797775	11.553983552225233	26.136336756229678
2	16.127968944115693	20.107595086320334	38.53842456524651	25.22601140431746
3	18.5123875810447	24.90755095691487	28.690052522345955	27.89000893969448
4	12.369723301480626	15.873214848262101	36.71033287829466	35.046728971962615
5	14.284973617200011	36.83662352663219	33.98419657154858	14.894206284619221
6	33.311916351701406	35.969437814076535	17.53289497431603	13.185750859906031
7	29.577685707966605	30.696130464657944	21.729671337900655	17.996512489474792
8	27.5667104851248	33.4109946046199	19.999330581717924	19.022964328537366
9	27.872600847271308	14.300377695303911	18.746419113810653	39.08060234361413
10	15.893509919866641	26.68887124190299	32.00917497424051	25.408443863989866
11	37.09791001479955	21.067142702443732	22.96358523195259	18.87136205080413
12	25.233562668301307	23.65068541249776	29.229084040557048	21.88666787864389
13	29.79225194948408	19.80616745871295	25.156877674797173	25.244702917005803
14	23.610999896296743	20.30043752272611	25.347701266361156	30.74086131461599
15	24.904322207357243	27.870563537275277	22.587902125720948	24.637212129646528
16	25.356652692641678	25.61644222242788	24.67908641775968	24.347818667170756
17	23.661308003859812	26.04461103197474	25.868096810403113	24.425984153762332
18	24.640069623293876	24.6775459626628	27.2345349389746	23.44784947506872
19	25.583912334060994	24.858854215208435	25.647183450796206	23.910049999934365
20	25.708770667828805	24.39565142056045	25.504520800407448	24.391057111203292
21	26.78554551516647	24.37366436863688	24.339994644347946	24.500795471848697
22	25.588833333552113	25.006858728568936	24.952317068848508	24.451990869030446
23	23.92400742991789	24.723708132872137	25.73685488687753	25.61542955033244
24	24.78708272316192	25.216494615285146	25.58459945952652	24.411823202026415
25	24.776427620575486	24.733564213715763	25.575012931226876	24.91499523448187
26	24.111316932666593	25.749655055617772	26.127682590778466	24.01134542093717
27	25.08985463109062	25.19716823359841	25.082766026154328	24.63021110915664
28	24.07008507292434	25.18190064017999	25.75986096398479	24.988153322910883
29	24.29428258626529	25.0792225556527	25.8023334882341	24.824161369847907
30	24.13898791865966	25.272368331241896	26.169608682183565	24.41903506791488
31	25.03412888642694	25.012273272618916	24.863353189344437	25.0902446516097
32	24.160006510839697	25.465062322256372	24.952924397468387	25.422006769435544
33	23.889210864681125	25.092299119376154	25.815989850214528	25.202500165728196
34	24.698314763459738	25.22059040711508	25.95349600433206	24.127598825093123
35	25.480060441601783	25.033181179083847	25.739799166893125	23.74695921242125
36	24.168224495368523	25.701744943183012	25.387663046902897	24.742367514545567
37	25.027960608359827	25.550876492873325	25.06189402273079	24.35926887603605
38	24.216342089649416	25.703257496600212	25.439478506508166	24.64092190724221
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	463.0
1	391.5
2	320.0
3	320.0
4	700.5
5	1081.0
6	1081.0
7	1194.0
8	1307.0
9	1307.0
10	1307.0
11	1307.0
12	1653.0
13	1999.0
14	2519.5
15	3040.0
16	3040.0
17	4456.5
18	5873.0
19	5873.0
20	7220.5
21	8568.0
22	9478.0
23	10388.0
24	10388.0
25	12973.5
26	15559.0
27	15559.0
28	20818.5
29	26078.0
30	33534.5
31	40991.0
32	40991.0
33	52387.5
34	63784.0
35	63784.0
36	69280.5
37	74777.0
38	84543.5
39	94310.0
40	94310.0
41	97734.0
42	101158.0
43	111280.5
44	121403.0
45	121403.0
46	124950.5
47	128498.0
48	128498.0
49	134215.0
50	139932.0
51	139258.5
52	138585.0
53	138585.0
54	131212.5
55	123840.0
56	123840.0
57	118755.0
58	113670.0
59	101220.5
60	88771.0
61	88771.0
62	84072.0
63	79373.0
64	65279.5
65	51186.0
66	51186.0
67	42505.0
68	33824.0
69	33824.0
70	27150.5
71	20477.0
72	16079.5
73	11682.0
74	11682.0
75	8842.0
76	6002.0
77	6002.0
78	5766.0
79	5530.0
80	4328.0
81	3126.0
82	3126.0
83	3250.5
84	3375.0
85	3375.0
86	2234.5
87	1094.0
88	981.0
89	868.0
90	868.0
91	619.5
92	371.0
93	320.5
94	270.0
95	270.0
96	241.5
97	213.0
98	213.0
99	422.0
100	631.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11918168907229917
2	0.05197791726060625
3	0.011944420380593861
4	0.0028876620700336807
5	2.625147336394255E-4
6	7.219155175084202E-4
7	6.562868340985638E-5
8	8.53172884328133E-4
9	5.906581506887074E-4
10	9.188015677379893E-4
11	0.0019032318188858348
12	0.0028220333866238244
13	0.016013398752004956
14	0.009581787777839032
15	0.025070157062565136
16	0.012010049064003717
17	0.021919980258892028
18	0.006300353607346211
19	0.008006699376002478
20	0.006562868340985638
21	0.006562868340985638
22	0.007744184642363053
23	0.010106817245117883
24	0.016079027435414815
25	0.018376031354759785
26	0.01903231818885835
27	0.009975559878298169
28	0.005906581506887074
29	0.011025618812855872
30	0.003412691537312532
31	0.0057753241400673614
32	0.007284783858494058
33	0.009516159094429174
34	0.013060107998561417
35	0.01771974452066122
36	0.015488369284726106
37	0.009975559878298169
38	0.006562868340985638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1523724.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.470787076915364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.510896030375	42.220795138114795
2	13.895650796321618	15.138140716432083
3	4.2460617537711505	6.938589771153065
4	1.713536307869659	3.733506854981275
5	0.7905790518187442	2.1531731599544224
6	0.44123602267323436	1.4420684065019247
7	0.28996618780422745	1.1056280532772247
8	0.18627000520462786	0.811701903385376
9	0.13076288812126735	0.6410481692774452
>10	0.6247886255611378	6.201915777999389
>50	0.07420018924981896	2.8196676019148756
>100	0.08149889143895937	9.60947809397757
>500	0.010065997771881637	3.7136616150539785
>1k	0.0044872520187906086	3.47062473797657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3566	0.23403188503954786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2332	0.15304608971178507	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1958	0.12850096211649878	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1824	0.11970671853957804	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1770	0.11616276963544579	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1720	0.11288133546495298	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1664	0.10920612919400102	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1624	0.10658098185760677	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1595	0.10467775003872092	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1570	0.10303703295347451	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1548	0.10159320191845767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.562868340985638E-5	0.0
6	0.0	0.0	0.0	6.562868340985638E-5	0.0
7	0.0	0.0	0.0	6.562868340985638E-5	0.0
8	0.0	0.0	0.0	6.562868340985638E-5	0.0
9	0.0	0.0	0.0	6.562868340985638E-5	0.0
10	0.0	0.0	0.0	6.562868340985638E-5	6.562868340985638E-5
11	0.0	0.0	0.0	6.562868340985638E-5	6.562868340985638E-5
12	0.0	0.0	0.0	6.562868340985638E-5	6.562868340985638E-5
13	0.0	0.0	0.0	1.3125736681971276E-4	6.562868340985638E-5
14	0.0	0.0	0.0	1.3125736681971276E-4	6.562868340985638E-5
15	0.0	0.0	0.0	1.9688605022956913E-4	6.562868340985638E-5
16	0.0	0.0	0.0	2.625147336394255E-4	6.562868340985638E-5
17	0.0	0.0	0.0	2.625147336394255E-4	6.562868340985638E-5
18	0.0	0.0	0.0	3.9377210045913827E-4	6.562868340985638E-5
19	0.0	0.0	0.0	5.906581506887074E-4	6.562868340985638E-5
20	0.0	6.562868340985638E-5	0.0	8.531728843281329E-4	1.3125736681971276E-4
21	0.0	6.562868340985638E-5	0.0	0.0011156876179675584	1.3125736681971276E-4
22	0.0	6.562868340985638E-5	0.0	0.0014438310350168404	1.3125736681971276E-4
23	0.0	6.562868340985638E-5	0.0	0.0019688605022956914	1.3125736681971276E-4
24	0.0	6.562868340985638E-5	0.0	0.002953290753443537	1.3125736681971276E-4
25	0.0	6.562868340985638E-5	0.0	0.0038720923211815264	1.3125736681971276E-4
26	0.0	6.562868340985638E-5	0.0	0.005250294672788511	1.3125736681971276E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	40	1.5951705E-4	23.997145	8
GACGCTA	35	0.0020670942	22.856672	23
GTACTAA	60	1.13066955E-4	18.683455	1
TATACTG	180	0.0	17.774496	5
CTAGCGG	380	0.0	17.683847	29
TCTAGCG	385	0.0	17.453041	28
GTCCTAA	525	0.0	17.082016	1
TAGCGGC	415	0.0	15.806905	30
TAGGACG	900	0.0	15.463812	4
TTAGGAC	695	0.0	15.423637	3
TCTATAC	135	7.921699E-9	15.406586	3
GTCCTAT	490	0.0	15.36074	1
ACCGTCG	355	0.0	15.322121	8
CATCGTT	335	0.0	15.282267	28
GTCTTAG	160	3.0013325E-10	15.01349	1
TACCGTC	375	0.0	14.931558	7
CGCCGGT	465	0.0	14.79394	7
CGGTCCA	490	0.0	14.694541	10
CTATTCC	460	0.0	14.258238	4
AGGACGT	985	0.0	14.129372	5
>>END_MODULE