FastQCFastQC Report
Thu 2 Feb 2017
SRR4062746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523724
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39970.26231784758919596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29970.19668916417933954No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19460.1277134179155805No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19430.12751653186535095No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19090.1252851566294158No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18580.12193809377551315No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17500.11485019596724866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16820.11038744549537842No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16810.11032181681196856No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16230.10651535317419689No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16200.10631846712396732No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16110.10572780897327863No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15250.10008374220003098No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15250.10008374220003098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC3600.019.1130317
ACCGTCG3650.018.4128078
ATACCGT3950.017.4194746
CGTCGTA4000.017.200610
GATATAC2850.016.8426931
CCGTCGT4000.016.8011389
GTATTAG3650.016.2197421
GTTCGCC600.00244182816.00055711
GTCGTAG4300.016.00055711
TTTAGAA4850.015.8350842
TCTAGCG3550.015.32347128
ATCGTTT4400.015.27225729
CATCGTT4650.015.13931928
CTAGCGG3500.015.08524929
CGCATCG5050.014.89063213
TTGAACG650.00416325714.76877715
CATAATA1205.4194425E-714.6666972
ATTTAGA4700.014.6388071
CGTTTAT4850.014.515017531
CGGCATC4550.014.41713925
Produced by FastQC (version 0.11.5)