##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523724
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18255340205969	32.0	32.0	32.0	32.0	32.0
2	31.26965513439442	32.0	32.0	32.0	32.0	32.0
3	31.364995235357583	32.0	32.0	32.0	32.0	32.0
4	31.465906555255415	32.0	32.0	32.0	32.0	32.0
5	31.382980776046054	32.0	32.0	32.0	32.0	32.0
6	34.905276808660886	36.0	36.0	36.0	36.0	36.0
7	34.94178210752079	36.0	36.0	36.0	36.0	36.0
8	34.87494060604151	36.0	36.0	36.0	32.0	36.0
9	34.99937849636811	36.0	36.0	36.0	36.0	36.0
10	34.833326114178156	36.0	36.0	36.0	32.0	36.0
11	35.01341647174948	36.0	36.0	36.0	36.0	36.0
12	34.90876694204462	36.0	36.0	36.0	32.0	36.0
13	34.96898519679417	36.0	36.0	36.0	36.0	36.0
14	34.906119480955866	36.0	36.0	36.0	32.0	36.0
15	34.87816166182327	36.0	36.0	36.0	32.0	36.0
16	34.8869598431212	36.0	36.0	36.0	32.0	36.0
17	34.85312300652874	36.0	36.0	36.0	32.0	36.0
18	34.863302671612445	36.0	36.0	36.0	32.0	36.0
19	34.85063239799334	36.0	36.0	36.0	32.0	36.0
20	34.83752306848222	36.0	36.0	36.0	32.0	36.0
21	34.81933736030935	36.0	36.0	36.0	32.0	36.0
22	34.81101892468715	36.0	36.0	36.0	32.0	36.0
23	34.74365370631427	36.0	36.0	36.0	32.0	36.0
24	34.72187154629054	36.0	36.0	36.0	32.0	36.0
25	34.69378378236478	36.0	36.0	36.0	32.0	36.0
26	34.64019993122114	36.0	36.0	36.0	32.0	36.0
27	34.607703232343916	36.0	36.0	36.0	32.0	36.0
28	34.56821379724937	36.0	36.0	36.0	32.0	36.0
29	34.544729885464825	36.0	36.0	36.0	32.0	36.0
30	34.514748734022696	36.0	36.0	36.0	32.0	36.0
31	34.508422785228824	36.0	36.0	36.0	32.0	36.0
32	34.47722750314362	36.0	36.0	36.0	32.0	36.0
33	34.4420826868908	36.0	36.0	36.0	32.0	36.0
34	34.42836301062397	36.0	36.0	36.0	32.0	36.0
35	34.39587484347559	36.0	36.0	36.0	32.0	36.0
36	34.3609787599329	36.0	36.0	36.0	32.0	36.0
37	34.33987716935613	36.0	36.0	36.0	32.0	36.0
38	33.90626386405937	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	6.0
21	22.0
22	95.0
23	344.0
24	974.0
25	2417.0
26	5144.0
27	10089.0
28	17906.0
29	28160.0
30	43201.0
31	62789.0
32	90141.0
33	144012.0
34	353134.0
35	765288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1976077459464	18.66237765689322	12.363939238007225	26.776075359153158
2	15.132071162493995	21.079473710462	37.76379449296592	26.02466063407809
3	17.503366751458927	25.544783700985217	29.045220788016728	27.90662875953913
4	11.83621421755412	16.843623705636592	36.61652191147729	34.703640165332
5	13.77276987170905	37.49425749020164	33.58390364659217	15.149068991497147
6	33.38874585472556	36.4380533732181	17.065765890519472	13.107434881536866
7	29.30140006129739	30.89434469216437	21.716733859720854	18.087521386817386
8	27.488101269810432	33.714097074736884	19.71152962672246	19.086272028730217
9	27.703206464383413	14.140641817924068	18.550842963577463	39.60530875411506
10	15.654129091239625	26.98926897650673	31.974853532128428	25.381748400125222
11	37.109868775981944	20.94379695127634	22.941560222990258	19.004774049751454
12	25.01657130781418	23.67275309473275	29.24583157775914	22.064844019693933
13	29.7906704766355	19.687449547883407	25.20571337816216	25.316166597318933
14	23.495199918095402	20.18915499132389	25.321908692125344	30.99373639845536
15	24.926233359847323	28.004415497819817	22.3898816321066	24.679469510226262
16	25.296330107243904	25.59887853632189	24.5498033435211	24.55498801291311
17	23.68749196048628	26.098821046331228	25.73569754102449	24.477989452158003
18	24.68983884220502	24.599993174616923	27.149864411140072	23.56030357203798
19	25.551018425909156	24.947628310638933	25.625769496312977	23.87558376713893
20	25.745541843535968	24.303285896920965	25.560403327636767	24.390768931906305
21	26.739768317469775	24.35337656516309	24.502353774275246	24.404501343091887
22	25.592101981723726	24.915404627084694	25.00420023573823	24.48829315545335
23	23.811233282711676	24.569311199812038	25.84808777454155	25.771367742934736
24	24.84045667063064	25.225368898829448	25.523520007560425	24.410654422979487
25	24.834763273902148	24.606834706833197	25.594284525468215	24.964117493796444
26	24.214703066108473	25.586868479375845	26.09056895511848	24.1078594993972
27	25.20143096875219	25.001263352984758	25.088352673025216	24.70895300523783
28	24.104759129606148	25.016800942952923	25.819636627105698	25.05880330033523
29	24.2961979991127	24.889415668454394	25.844313012067804	24.970073320365106
30	24.192110907224667	25.084857887648944	26.21098046627867	24.51205073884772
31	25.011550648280135	24.78355660211429	24.978867563942025	25.22602518566355
32	24.232997576989007	25.377824330390542	24.98168959732865	25.407488495291798
33	23.858848452869417	24.847544568438902	26.017441478903002	25.27616549978868
34	24.77043086543232	24.95556938133153	26.038770801011207	24.235228952224944
35	25.450344025558437	24.878193163591305	25.77310589056811	23.89835692028215
36	24.267912036563054	25.4527722868446	25.489261834820482	24.79005384177187
37	25.21769034287049	25.31147373146318	25.067466286545333	24.403369639120996
38	24.46875186631691	25.348964345881765	25.455151625328227	24.7271321624731
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	228.5
2	180.0
3	180.0
4	601.5
5	1023.0
6	1023.0
7	1118.5
8	1214.0
9	1261.5
10	1309.0
11	1309.0
12	1631.5
13	1954.0
14	2584.0
15	3214.0
16	3214.0
17	4887.5
18	6561.0
19	6561.0
20	7659.0
21	8757.0
22	9524.5
23	10292.0
24	10292.0
25	12903.5
26	15515.0
27	15515.0
28	20775.0
29	26035.0
30	33118.0
31	40201.0
32	40201.0
33	51506.0
34	62811.0
35	62811.0
36	68619.0
37	74427.0
38	84346.5
39	94266.0
40	94266.0
41	97431.5
42	100597.0
43	111179.5
44	121762.0
45	121762.0
46	125728.5
47	129695.0
48	129695.0
49	135695.5
50	141696.0
51	140317.0
52	138938.0
53	138938.0
54	131289.5
55	123641.0
56	123641.0
57	118652.5
58	113664.0
59	101527.0
60	89390.0
61	89390.0
62	84601.5
63	79813.0
64	65591.0
65	51369.0
66	51369.0
67	42613.0
68	33857.0
69	33857.0
70	27403.5
71	20950.0
72	16381.5
73	11813.0
74	11813.0
75	8753.5
76	5694.0
77	5694.0
78	5660.5
79	5627.0
80	4293.0
81	2959.0
82	2959.0
83	2878.5
84	2798.0
85	2798.0
86	1771.0
87	744.0
88	616.5
89	489.0
90	489.0
91	295.0
92	101.0
93	74.0
94	47.0
95	47.0
96	36.0
97	25.0
98	25.0
99	22.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009188015677379893
2	0.0
3	0.0
4	1.3125736681971276E-4
5	0.0
6	6.562868340985638E-5
7	3.2814341704928186E-4
8	0.002100117869115404
9	0.003412691537312532
10	7.219155175084202E-4
11	0.004594007838689946
12	3.937721004591382E-4
13	1.3125736681971276E-4
14	0.0
15	0.0
16	6.562868340985638E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.562868340985638E-5
22	0.0
23	1.3125736681971276E-4
24	0.0
25	6.562868340985638E-5
26	6.562868340985638E-5
27	6.562868340985638E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.562868340985638E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1523724.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.669365652044384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11846894601466	40.09111473791432
2	14.561452426956595	15.338849246004521
3	4.625032814280765	7.307926333441725
4	1.873339277746834	3.94670365639939
5	0.8652721575776913	2.278666782799639
6	0.47106618151904367	1.4886454176443293
7	0.2971128000955886	1.0954119895696126
8	0.19404956842898766	0.8176374139366208
9	0.13952941425566417	0.6614033164782437
>10	0.674869460405104	6.400668051184738
>50	0.07714549851501395	2.8397580782611778
>100	0.08659716391126694	9.909801723298429
>500	0.011797213183855	4.363079001157156
>1k	0.004267077109053935	3.460334251910068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3997	0.26231784758919596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2997	0.19668916417933954	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1946	0.1277134179155805	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1943	0.12751653186535095	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1909	0.1252851566294158	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1858	0.12193809377551315	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1750	0.11485019596724866	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1682	0.11038744549537842	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1681	0.11032181681196856	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1623	0.10651535317419689	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1620	0.10631846712396732	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1611	0.10572780897327863	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1525	0.10008374220003098	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1525	0.10008374220003098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
6	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
7	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
8	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
9	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
10	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
11	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
12	0.0	1.3125736681971276E-4	0.0	6.562868340985638E-5	0.0
13	0.0	1.3125736681971276E-4	0.0	1.3125736681971276E-4	0.0
14	0.0	1.3125736681971276E-4	0.0	1.3125736681971276E-4	0.0
15	0.0	1.3125736681971276E-4	0.0	2.625147336394255E-4	0.0
16	0.0	1.3125736681971276E-4	0.0	3.281434170492819E-4	0.0
17	0.0	1.3125736681971276E-4	0.0	3.281434170492819E-4	0.0
18	0.0	1.3125736681971276E-4	0.0	5.25029467278851E-4	0.0
19	0.0	1.3125736681971276E-4	0.0	7.219155175084202E-4	0.0
20	0.0	1.9688605022956913E-4	0.0	9.188015677379893E-4	0.0
21	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.0011813163013774147	0.0
22	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.001378202351606984	0.0
23	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.001903231818885835	0.0
24	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.002953290753443537	0.0
25	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.003609577587542101	0.0
26	6.562868340985638E-5	1.9688605022956913E-4	0.0	0.004922151255739228	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	360	0.0	19.113031	7
ACCGTCG	365	0.0	18.412807	8
ATACCGT	395	0.0	17.419474	6
CGTCGTA	400	0.0	17.2006	10
GATATAC	285	0.0	16.842693	1
CCGTCGT	400	0.0	16.801138	9
GTATTAG	365	0.0	16.219742	1
GTTCGCC	60	0.002441828	16.000557	11
GTCGTAG	430	0.0	16.000557	11
TTTAGAA	485	0.0	15.835084	2
TCTAGCG	355	0.0	15.323471	28
ATCGTTT	440	0.0	15.272257	29
CATCGTT	465	0.0	15.139319	28
CTAGCGG	350	0.0	15.085249	29
CGCATCG	505	0.0	14.890632	13
TTGAACG	65	0.004163257	14.768777	15
CATAATA	120	5.4194425E-7	14.666697	2
ATTTAGA	470	0.0	14.638807	1
CGTTTAT	485	0.0	14.5150175	31
CGGCATC	455	0.0	14.417139	25
>>END_MODULE