FastQCFastQC Report
Thu 2 Feb 2017
SRR4062745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT802.9250457038391224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT762.7787934186471666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA602.1937842778793417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT471.7184643510054844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331.206581352833638No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA190.6946983546617916No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC160.5850091407678245No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT160.5850091407678245No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA140.5118829981718465No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA120.43875685557586835No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.43875685557586835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.43875685557586835No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100.3656307129798903No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA100.3656307129798903No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA100.3656307129798903No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA100.3656307129798903No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA90.3290676416819013No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.29250457038391225No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70.25594149908592323No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA70.25594149908592323No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT70.25594149908592323No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.21937842778793418No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG60.21937842778793418No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT60.21937842778793418No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG60.21937842778793418No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA60.21937842778793418No Hit
ATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAA50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50.18281535648994515No Hit
GGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA50.18281535648994515No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.18281535648994515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA50.18281535648994515No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50.18281535648994515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA50.18281535648994515No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.14625228519195613No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.14625228519195613No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAA40.14625228519195613No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA40.14625228519195613No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA40.14625228519195613No Hit
TCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAA40.14625228519195613No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG40.14625228519195613No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAG40.14625228519195613No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA40.14625228519195613No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14625228519195613No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14625228519195613No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14625228519195613No Hit
TTTTTTTGCGTTGATACCACCCCATGTACTCTGCGTTG30.10968921389396709No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.10968921389396709No Hit
ACGCAGAGTACATGGGTGTGGTATCAACGCAAAAAAAA30.10968921389396709No Hit
GTACATGGGACGCAGAGTACTTTTTTTTTAAAAAAAAA30.10968921389396709No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGT30.10968921389396709No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT30.10968921389396709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT30.10968921389396709No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACG30.10968921389396709No Hit
GGTATCAACGCAGAGTACATGGGACGCAGAGTACTTTT30.10968921389396709No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG30.10968921389396709No Hit
CGGCAGGAGAGCCTCCCCAGCTCGAGGAAGCCAAGTTG30.10968921389396709No Hit
TCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAA30.10968921389396709No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.10968921389396709No Hit
CTTTTAATTTCTTGTCTTTCGCATTTATTTCTTTAATT30.10968921389396709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGT30.10968921389396709No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG30.10968921389396709No Hit
TTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA30.10968921389396709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.10968921389396709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG1150.0097315019.739136
GTATCAA1150.0097315019.739131
CAGAGTA1150.0097315019.7391310
Produced by FastQC (version 0.11.5)