##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062745_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2735
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83473491773309	32.0	32.0	32.0	14.0	32.0
2	29.417184643510055	32.0	32.0	32.0	14.0	32.0
3	29.80255941499086	32.0	32.0	32.0	21.0	32.0
4	29.717733089579525	32.0	32.0	32.0	21.0	32.0
5	30.089213893967095	32.0	32.0	32.0	21.0	32.0
6	33.22522851919561	36.0	36.0	36.0	21.0	36.0
7	33.08921389396709	36.0	36.0	36.0	21.0	36.0
8	33.112614259597805	36.0	36.0	36.0	21.0	36.0
9	32.83729433272395	36.0	36.0	36.0	14.0	36.0
10	32.80987202925046	36.0	36.0	36.0	14.0	36.0
11	33.56343692870201	36.0	36.0	36.0	21.0	36.0
12	33.011334552102376	36.0	36.0	36.0	21.0	36.0
13	33.2800731261426	36.0	36.0	36.0	21.0	36.0
14	33.19012797074954	36.0	36.0	36.0	21.0	36.0
15	33.34808043875685	36.0	36.0	36.0	21.0	36.0
16	33.29287020109689	36.0	36.0	36.0	21.0	36.0
17	32.9327239488117	36.0	36.0	36.0	21.0	36.0
18	33.288117001828155	36.0	36.0	36.0	21.0	36.0
19	33.09981718464351	36.0	36.0	36.0	21.0	36.0
20	32.86691042047532	36.0	36.0	36.0	14.0	36.0
21	32.890310786106035	36.0	36.0	36.0	14.0	36.0
22	32.741499085923216	36.0	36.0	36.0	14.0	36.0
23	33.055941499085925	36.0	36.0	36.0	21.0	36.0
24	33.11627056672761	36.0	36.0	36.0	21.0	36.0
25	33.0745886654479	36.0	36.0	36.0	21.0	36.0
26	33.03692870201097	36.0	36.0	36.0	21.0	36.0
27	33.088848263254114	36.0	36.0	36.0	21.0	36.0
28	33.03144424131627	36.0	36.0	36.0	21.0	36.0
29	32.960877513711154	36.0	36.0	36.0	21.0	36.0
30	32.948080438756854	36.0	36.0	36.0	21.0	36.0
31	32.86581352833638	36.0	36.0	36.0	21.0	36.0
32	32.65594149908593	36.0	36.0	36.0	14.0	36.0
33	32.65557586837294	36.0	36.0	36.0	21.0	36.0
34	32.30054844606947	36.0	32.0	36.0	14.0	36.0
35	32.4146252285192	36.0	36.0	36.0	14.0	36.0
36	32.247531992687385	36.0	32.0	36.0	14.0	36.0
37	32.312248628884824	36.0	36.0	36.0	14.0	36.0
38	31.484826325411333	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	2.0
17	7.0
18	13.0
19	13.0
20	24.0
21	21.0
22	26.0
23	44.0
24	40.0
25	47.0
26	48.0
27	77.0
28	82.0
29	102.0
30	125.0
31	151.0
32	200.0
33	239.0
34	463.0
35	1006.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.28341266935189	21.750274624679605	16.03808128890516	15.928231417063346
2	15.080527086383603	25.43923865300146	37.920937042459734	21.559297218155198
3	19.26873857404022	32.21206581352833	31.480804387568558	17.038391224862888
4	12.248628884826324	23.254113345521024	30.091407678244973	34.40585009140768
5	11.005484460694698	42.85191956124314	28.26325411334552	17.879341864716636
6	24.82632541133455	36.819012797074954	19.89031078610603	18.46435100548446
7	21.46252285191956	38.098720292504574	21.206581352833638	19.23217550274223
8	28.994515539305297	29.90859232175503	20.036563071297987	21.060329067641682
9	27.824497257769654	18.574040219378425	21.681901279707496	31.919561243144422
10	19.122486288848265	32.65082266910421	25.59414990859232	22.632541133455213
11	28.153564899451556	28.957952468007313	20.548446069469836	22.340036563071298
12	26.06946983546618	33.49177330895795	27.422303473491773	13.016453382084094
13	29.91953182150695	25.237746891002192	26.371616678858818	18.471104608632043
14	24.16819012797075	27.276051188299817	30.018281535648995	18.537477148080438
15	24.185876326381265	29.710940358580313	29.56458104646908	16.53860226856934
16	17.55669348939283	32.00438917337235	29.151426481346014	21.28749085588881
17	16.648371752652764	31.430662275887304	30.698865715331138	21.222100256128794
18	17.5932699341624	28.383321141185075	35.69861009509875	18.324798829553767
19	20.629114850036576	29.334308705193855	35.11338697878566	14.923189465983908
20	21.945866861741038	24.83540599853694	36.6861741038771	16.532553035844916
21	24.753199268738573	26.617915904936012	32.35831809872029	16.270566727605118
22	22.05559619604974	25.53035844915874	34.345281638624726	18.06876371616679
23	19.96343692870201	27.605118829981716	35.79524680073126	16.63619744058501
24	18.983174835406	30.504754937820046	34.784198975859546	15.72787125091441
25	18.434528163862474	29.809802487198244	34.52816386247257	17.227505486466715
26	18.324798829553767	31.565471836137526	34.01609363569861	16.093635698610097
27	18.105340160936358	31.236283833211413	34.345281638624726	16.313094367227503
28	16.63619744058501	31.37111517367459	35.6855575868373	16.30712979890311
29	16.563071297989033	30.96892138939671	35.90493601462523	16.563071297989033
30	16.160877513711153	33.016453382084094	34.771480804387565	16.051188299817184
31	15.319926873857403	33.089579524680076	34.62522851919561	16.96526508226691
32	16.2033650329188	33.50402340892465	32.66276517922458	17.62984637893197
33	15.837600585223116	33.72348207754206	33.68690563277249	16.752011704462326
34	16.715435259692757	32.88222384784199	32.918800292611564	17.483540599853693
35	16.898317483540602	34.56474030724214	32.00438917337235	16.532553035844916
36	15.837600585223116	36.5764447695684	32.33357717629846	15.252377468910023
37	17.300658376005853	34.308705193855154	33.46744696415509	14.923189465983908
38	16.19744058500914	35.42961608775137	32.02925045703839	16.343692870201096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	57.0
2	26.0
3	26.0
4	32.0
5	38.0
6	38.0
7	38.5
8	39.0
9	40.0
10	41.0
11	41.0
12	45.0
13	49.0
14	63.0
15	77.0
16	77.0
17	110.5
18	144.0
19	144.0
20	133.0
21	122.0
22	112.5
23	103.0
24	103.0
25	81.0
26	59.0
27	59.0
28	70.5
29	82.0
30	99.0
31	116.0
32	116.0
33	101.5
34	87.0
35	87.0
36	91.5
37	96.0
38	97.0
39	98.0
40	98.0
41	134.5
42	171.0
43	187.5
44	204.0
45	204.0
46	223.0
47	242.0
48	242.0
49	237.0
50	232.0
51	187.0
52	142.0
53	142.0
54	115.5
55	89.0
56	89.0
57	88.0
58	87.0
59	80.5
60	74.0
61	74.0
62	67.5
63	61.0
64	56.5
65	52.0
66	52.0
67	44.5
68	37.0
69	37.0
70	30.5
71	24.0
72	19.0
73	14.0
74	14.0
75	11.5
76	9.0
77	9.0
78	7.0
79	5.0
80	7.0
81	9.0
82	9.0
83	6.0
84	3.0
85	3.0
86	1.5
87	0.0
88	0.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	7.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14625228519195613
2	0.10968921389396709
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.03656307129798903
14	0.0
15	0.07312614259597806
16	0.03656307129798903
17	0.07312614259597806
18	0.03656307129798903
19	0.03656307129798903
20	0.03656307129798903
21	0.0
22	0.03656307129798903
23	0.0
24	0.03656307129798903
25	0.03656307129798903
26	0.03656307129798903
27	0.03656307129798903
28	0.0
29	0.0
30	0.0
31	0.0
32	0.03656307129798903
33	0.03656307129798903
34	0.03656307129798903
35	0.03656307129798903
36	0.03656307129798903
37	0.03656307129798903
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2735.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.18647166361974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37506160670281	67.78793418647166
2	5.076392311483489	7.53199268738574
3	1.1828486939379004	2.6325411334552102
4	0.5914243469689502	1.7550274223034734
5	0.4928536224741252	1.8281535648994516
6	0.2464268112370626	1.0968921389396709
7	0.14785608674223755	0.7678244972577696
8	0.04928536224741252	0.29250457038391225
9	0.04928536224741252	0.3290676416819013
>10	0.6407097092163627	8.080438756855576
>50	0.14785608674223755	7.897623400365631
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	80	2.9250457038391224	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	76	2.7787934186471666	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	2.1937842778793417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	47	1.7184643510054844	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	1.206581352833638	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	19	0.6946983546617916	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	16	0.5850091407678245	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	16	0.5850091407678245	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	14	0.5118829981718465	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	12	0.43875685557586835	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.43875685557586835	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	12	0.43875685557586835	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.3656307129798903	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	10	0.3656307129798903	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	10	0.3656307129798903	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	10	0.3656307129798903	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	9	0.3290676416819013	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.29250457038391225	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.25594149908592323	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	7	0.25594149908592323	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	7	0.25594149908592323	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	6	0.21937842778793418	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	6	0.21937842778793418	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	6	0.21937842778793418	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	6	0.21937842778793418	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	6	0.21937842778793418	No Hit
ATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAA	5	0.18281535648994515	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	5	0.18281535648994515	No Hit
GGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.18281535648994515	No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.18281535648994515	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.18281535648994515	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA	5	0.18281535648994515	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	5	0.18281535648994515	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5	0.18281535648994515	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.18281535648994515	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	5	0.18281535648994515	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4	0.14625228519195613	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	4	0.14625228519195613	No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAA	4	0.14625228519195613	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	4	0.14625228519195613	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	4	0.14625228519195613	No Hit
TCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAA	4	0.14625228519195613	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	4	0.14625228519195613	No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAG	4	0.14625228519195613	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	4	0.14625228519195613	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.14625228519195613	No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.14625228519195613	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.14625228519195613	No Hit
TTTTTTTGCGTTGATACCACCCCATGTACTCTGCGTTG	3	0.10968921389396709	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3	0.10968921389396709	No Hit
ACGCAGAGTACATGGGTGTGGTATCAACGCAAAAAAAA	3	0.10968921389396709	No Hit
GTACATGGGACGCAGAGTACTTTTTTTTTAAAAAAAAA	3	0.10968921389396709	No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGT	3	0.10968921389396709	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	3	0.10968921389396709	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	3	0.10968921389396709	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	3	0.10968921389396709	No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACG	3	0.10968921389396709	No Hit
GGTATCAACGCAGAGTACATGGGACGCAGAGTACTTTT	3	0.10968921389396709	No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.10968921389396709	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAA	3	0.10968921389396709	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	3	0.10968921389396709	No Hit
CGGCAGGAGAGCCTCCCCAGCTCGAGGAAGCCAAGTTG	3	0.10968921389396709	No Hit
TCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAA	3	0.10968921389396709	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3	0.10968921389396709	No Hit
CTTTTAATTTCTTGTCTTTCGCATTTATTTCTTTAATT	3	0.10968921389396709	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	3	0.10968921389396709	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGT	3	0.10968921389396709	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	3	0.10968921389396709	No Hit
TTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10968921389396709	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	3	0.10968921389396709	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	3	0.10968921389396709	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	3	0.10968921389396709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.03656307129798903	0.0
7	0.0	0.0	0.0	0.07312614259597806	0.0
8	0.0	0.0	0.0	0.07312614259597806	0.0
9	0.0	0.0	0.0	0.07312614259597806	0.0
10	0.0	0.0	0.0	0.07312614259597806	0.0
11	0.0	0.0	0.0	0.07312614259597806	0.0
12	0.0	0.0	0.0	0.07312614259597806	0.0
13	0.0	0.0	0.0	0.07312614259597806	0.0
14	0.0	0.0	0.0	0.07312614259597806	0.0
15	0.0	0.0	0.0	0.07312614259597806	0.0
16	0.0	0.0	0.0	0.07312614259597806	0.0
17	0.0	0.0	0.0	0.07312614259597806	0.0
18	0.0	0.0	0.0	0.07312614259597806	0.0
19	0.0	0.0	0.0	0.07312614259597806	0.0
20	0.0	0.0	0.0	0.07312614259597806	0.0
21	0.0	0.0	0.0	0.07312614259597806	0.0
22	0.0	0.0	0.0	0.07312614259597806	0.0
23	0.0	0.0	0.0	0.07312614259597806	0.0
24	0.0	0.0	0.0	0.07312614259597806	0.0
25	0.0	0.0	0.0	0.07312614259597806	0.0
26	0.0	0.0	0.0	0.14625228519195613	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	115	0.009731501	9.73913	6
GTATCAA	115	0.009731501	9.73913	1
CAGAGTA	115	0.009731501	9.73913	10
>>END_MODULE